The Anaphylactic Antibodies of Mammals Including Man*

Kj, Bloch

doi:10.1159/000385905

Cited by 51 publications

(38 citation statements)

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“…Peaks I and IV contained exclusively 7 S slow IgG and 19 S IgM, respectively, while 7 S fast IgG predominated in peak II of which a minor portion was 7 S IgA in the case of rabbit sera. Peak I of the guinea pig serum was considered to be 7 S y2 [2], and an Ouchterlony test with a rabbit antiserum against this fraction suggested that peak II was 7' S yi contaminated by 7 S y2. In both species, the greater part of peak IIA was albumin and peak III consisted of albumin and a trace of IgG.…”

Section: Identification Of Deae-cellulose Fractions Ofmentioning

confidence: 99%

Complement Requirement of Neutralizing Antibodies in Different Classes of Immunoglobulin Appearing in Rabbits and Guinea Pigs after Primary and Booster Immunizations with Herpes Simplex Virus

Shinkai

Yoshino

1975

Japanese Journal of Microbiology

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Rabbits and guinea pigs were immunized with herpes simplex virus and bled periodically. The sera were fractionated into slow IgG, fast IgG and IgM by DEAE‐cellulose column chromatography, and complement‐requiring (CRN) and nonrequiring neutralizing (N) antibody activities were estimated. In early sera of rabbits, the two IgG and IgM fractions possessed about equal CRN activities, although some animals showed a slightly lower activity in fast IgG. In guinea pigs, the early CRN activity resided mainly in slow IgG (7 S γ2). The early IgG antibody of guinea pigs differed from that of rabbits in that it resembled IgM in resistances to heating at 70 C and to 2‐mercaptoethanol. The level of CRN IgM antibody in rabbits declined following a peak reached in 2 to 3 weeks, whereas such a decline was never observed in guinea pigs. N IgG antibody was developed a few weeks after the first immunization in rabbits and much retarded in guinea pigs. In both species, booster immunization quickly evoked N antibody in the two IgG fractions and also CRN IgM antibody, but in the case of rabbits the IgM antibody disappeared soon. It is concluded that IgG plays an important role in humoral immunity from the initial stage of the immunization course.

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Section: Identification Of Deae-cellulose Fractions Ofmentioning

confidence: 99%

Complement Requirement of Neutralizing Antibodies in Different Classes of Immunoglobulin Appearing in Rabbits and Guinea Pigs after Primary and Booster Immunizations with Herpes Simplex Virus

Shinkai

Yoshino

1975

Japanese Journal of Microbiology

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“…In general, the IgE production elicited by immuniza tion is transient and although secondary homocytotropic antibody (IgE) responses can be obtained by booster injection, the titers are usually low. This is a fundamental difference between the prolonged, persistent pat tern of IgE formation observed in humans with atopic diseases and in ani mals infected with helminth parasites [11]. Thus, it was of interest when Levine and V az found in guinea pigs and mice that periodic injections of microgram quantities of antigen combined with aluminum hydroxide gel induced the prolonged synthesis of high titers of homocytotropic antibod ies [7,12].…”

Section: Introductionmentioning

confidence: 99%

Characterization of a Rabbit Homocytotropic Antibody Responsible for Passive Cutaneous Anaphylactic Reactions with a Short (4-Hour) Latent Period

Kravis¹,

Zvaifler²

1974

Int Arch Allergy Immunol

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Adult New Zealand rabbits were immunized intraperitoneally with 1·0 μgof ovalbumin (EA) in alumina gel or subcutaneously with 1.5 μg of bovine γ-globulin (BGG) and alumina. Immunizations were repeated at 30 and 60 days and every 2 months thereafter. The rabbit sera were examined for homocytotropic antibody by a 72-hour passive cutaneous anaphylactic (PCA) reaction in rabbit skin. Seven of 20 rabbits immunized with EA and 3 of 9 given BGG developed titers of 1:20 or greater; 2 in each group had titers in excess of 1:200. Rabbit sera with 72-hour PCA titers greater than 1:200 also caused vascular permeability of rabbit skin after a 4-hour latent period. The 4-hour anaphylactic antibody had physical-chemical characteristics similar to the 72-hour PCA antibody. It eluted from DE-52 cellulose with 0.10 m saline, was excluded from Sephadex G-200 between the first and second peak, and appeared in a sucrose density gradient slightly in advance of the bulk of the IgG. Absorption with monospecific antisera to rabbit IgE eliminated both the 4- and 72-hour PCA reactions, but there was little effect from an antibody to rabbit IgG. These findings suggest that the same antibody was responsible for the 4- and 72-hour anaphylactic reactions and that it is rabbit IgE.

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“…It is well established that homocytotropic antibodies detected by this procedure are of the [gE class (15). Details of their DNP specificity have been previously described (9).…”

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confidence: 99%

HAPTEN-SPECIFIC IgE ANTIBODY RESPONSES IN MICE

Hamaoka

Katz

Benacerraf

1973

The Journal of Experimental Medicine

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The present studies have established conditions for the demonstration of cooperative interactions between specific T and B lymphocyte populations in the development of IgE antibody responses in vivo in mice. This has been accomplished by utilizing a system which permits the successful adoptive transfer to irradiated recipients of DNP-specific secondary IgE responses with spleen cells from suitably primed syngeneic donor mice. Thus, adoptively transferred DNP-KLH or DNP-ASC-primed spleen cells produced high levels of anti-DNP antibodies of both IgE and IgG antibody classes in response to challenge with the appropriate homologous priming conjugate but failed to develop more than meager responses to the reciprocal heterologous conjugate. However, when spleen cells from donors primed to the second carrier were concomitantly transferred with hapten-primed lymphocytes, secondary IgE ant-DNP responses were consistently obtained upon challenge with the heterologous conjugate. Moreover, we have been able to elicit augmented primary IgE anti-DNP antibody responses to either DNP-ASC or DNP-KLH after adoptive transfer of spleen cells from donors primed only to the carrier, ASC or KLH, respectively. This adoptive transfer system has enabled us to provide direct proof for the participation of θ-bearing T lymphocytes in antibody responses of the IgE class. Thus, the capacity of ASC-primed spleen cells to effectively cooperate with the DNP-KLH-primed lymphocytes in the adoptive secondary response to DNP-ASC could be abolished by in vitro treatment of such cells with anti-θ serum plus complement. This was true not only for the anti-DNP response of the IgG antibody class, but for the IgE antibody class as well. These studies have, furthermore, demonstrated the capacity to stimulate secondary anti-DNP antibody production in vivo by the concomitant administration of the DNP and relevant carrier determinants on separate molecules. This was more readily seen in the IgE than in the IgG antibody class. Thus, DNP-ASC-primed cells developed significant IgE, but more variable IgG, anti-DNP responses upon challenge with DNP-KLH plus unconjugated ASC. Antibody responses of both classes elicited in this manner were appreciably improved by the transfer of additional carrier (ASC)-primed cells. These and other results presented herein suggest that IgE B lymphocyte precursors may be inherently more sensitive than IgG B cells to at least certain of the functions of T lymphocytes concerned with regulatory mechanisms involved in antibody production.

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The Anaphylactic Antibodies of Mammals Including Man*

Cited by 51 publications

References 0 publications

Complement Requirement of Neutralizing Antibodies in Different Classes of Immunoglobulin Appearing in Rabbits and Guinea Pigs after Primary and Booster Immunizations with Herpes Simplex Virus

Complement Requirement of Neutralizing Antibodies in Different Classes of Immunoglobulin Appearing in Rabbits and Guinea Pigs after Primary and Booster Immunizations with Herpes Simplex Virus

Characterization of a Rabbit Homocytotropic Antibody Responsible for Passive Cutaneous Anaphylactic Reactions with a Short (4-Hour) Latent Period

HAPTEN-SPECIFIC IgE ANTIBODY RESPONSES IN MICE

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