The analytical environmental immunochemical consortium

Reeves, Stuart; Berberich, Sharon A.; Dautlick, J. X.; Mihaliak, Charles A

doi:10.1080/09540109609354911

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“…Moreover, expectations for analytical performance and adequate design of analytical strategies by chemists as well as by IA developing laboratories should be realistic. Guidelines for the adequate promotion and use of immunochemical methods as analytical tools have been suggested by the Analytical Environmental Immunochemical Consortium and others (Mihaliak and Berberich, 1995;Reeves et al, 1996;Williams et al, 1996).…”

Section: Introductionmentioning

confidence: 99%

Development of Monoclonal ELISAs for Azinphos-methyl. 2. Assay Optimization and Water Sample Analysis

Mercader

Montoya

1999

J. Agric. Food Chem.

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Two enzyme-linked immunosorbent assays (ELISA) for the insecticide azinphos-methyl have been optimized and characterized. Both ELISAs are based on monoclonal antibodies produced from an immunogen with a hapten containing a phthalimido moiety and on protein conjugates of heterologous ligands containing a 1,2,3-benzotriazine group. Assay I was performed in the conjugate-coated ELISA format and assay II in the antibody-coated format. Several physicochemical factors (ionic strength, pH, incubation times, and Tween 20 and BSA concentrations) that influence assay performance were studied and optimized. Regarding specificity, both monoclonal immunoassays highly cross-reacted with azinphos-ethyl and phosmet. Finally, both assays were applied to the analysis of azinphos-methyl in spiked real water samples. For assay I the sensitivity, estimated as the I(50) value, was 0.40 nM, with a practical working range between 0.10 and 1.75 ng/mL and a limit of detection of 0.05 ng/mL. For assay II the sensitivity was 1.01 nM, with a practical working range between 0.32 and 2.54 ng/mL and a limit of detection of 0.08 ng/mL.

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Section: Introductionmentioning

confidence: 99%