The Analysis of the Relationship Between A1298C and C677T Polymorphisms of theMTHFRGene with Prostate Cancer in Eskisehir Population

Abstract

Prostate cancer is the most common cause of cancer deaths in men and is a major health problem worldwide. Methylene tetrahydrofolate reductase (MTHFR) plays an important role for folate metabolism and is also an important source for DNA methylation and DNA synthesis (nucleotide synthesis). The objective of this study was to investigate the relationship between the A1298C and C677T polymorphisms of the MTHFR gene and prostate cancer in the Turkish population. In our study, 93 prostate cancer patients between the ages of 50-89 and a control group of 166 benign prostate hyperplasia patients were evaluated. C677T and A1298C polymorphism ratios were compared among these two groups, and an analysis was made to see if there is a statistically meaningful difference. In this study, it has been observed that C677T polymorphism of the MTHFR gene produces no statistically significant difference for T allele frequency and the genotype frequency in prostate cancer patients and male controls with benign prostate hyperplasia not having prostate cancer, whereas it has been observed that A1298C polymorphism produces a statistically significant difference for C allele frequency in prostate cancer patients and controls and that it also produces a statistically marginal significance for genotype frequencies.

The MTHFR C677T Polymorphism and Prostate Cancer Risk: New Findings from a Meta-analysis of 7306 Cases and 8062 Controls

“…Our work included more studies, which were published during the past 3 years, than the previous two; however, we did not contain unpublished data from GWAS as Collin did. Interestingly, the newly published 5 studies (Muslumanoglu et al, 2009;Cai et al, 2010;Safarinejad et al, 2010;Wu et al, 2010;Kucukhuseyin et al, 2011) were all conducted in Asia, which allowed us to conduct a subgroup analysis, and no such study was included in the previous analysis.…”

“…We included 15 independent studies (Kimura et al, Heijmans et al, 2003;Cicek et al, 2004;Singal et al, 2004;Van et al, 2006;Johansson et al, 2007;Reljic et al, 2007;Marchal et al, 2008;Stevens et al, 2008;Collin et al, 2009;Muslumanoglu et al, 2009;Cai et al, 2010;Safarinejad et al, 2010;Wu et al, 2010;Kucukhuseyin et al, 2011) published during 2000 to 2011 with a total of 7306 cases and 8062 controls in our meta-analysis. According to the background of control population, 6 were hospital-based case-control studies (Kimura et al, 2000;Singal et al, 2004;Marchal et al, 2008;Muslumanoglu et al, 2009;Cai et al, 2010;Wu et al, 2010), 5 were population-based studies (Cicek et al, 2004;Johansson et al, 2007;Reljic et al, 2007;Safarinejad et al, 2010;Kucukhuseyin et al, 2011) and the rest 4 were populationbased studies nested in cohort (Heijmans et al, 2003;Van et al, 2006;Stevens et al, 2008;Collin et al, 2009).…”

“…According to the background of control population, 6 were hospital-based case-control studies (Kimura et al, 2000;Singal et al, 2004;Marchal et al, 2008;Muslumanoglu et al, 2009;Cai et al, 2010;Wu et al, 2010), 5 were population-based studies (Cicek et al, 2004;Johansson et al, 2007;Reljic et al, 2007;Safarinejad et al, 2010;Kucukhuseyin et al, 2011) and the rest 4 were populationbased studies nested in cohort (Heijmans et al, 2003;Van et al, 2006;Stevens et al, 2008;Collin et al, 2009). One study (Cicek et al, 2004) used sibling as control population.…”

“…Two of the 15 studies were conducted in mixed ethnicities (Cicek et al, 2004;Singal et al, 2004) (Cicek: Caucasian/African-American/Latino; Singal: Caucasian/ African-American), two in yellow Asian (Cai et al, 2010;Wu et al, 2010) and the rests in Caucasian (Kimura et al, 2000;Heijmans et al, 2003;Van et al, 2006;Johansson et al, 2007;Reljic et al, 2007;Marchal et al, 2008;Stevens et al, 2008;Collin et al, 2009;Muslumanoglu et al, 2009;Safarinejad et al, 2010;Kucukhuseyin et al, 2011). In terms of the residential region of the participants, the most recent 5 studies were all conducted in Asia (Muslumanoglu et al, 2009;Cai et al, 2010;Safarinejad et al, 2010;Wu et al, 2010;Kucukhuseyin et al, 2011), 3 of the rest 10 were in the USA (Cicek et al, 2004;Singal et al, 2004;Stevens et al, 2008) and 7 in Europe (Kimura et al, 2000;Heijmans et al, 2003;Van et al, 2006;Johansson et al, 2007;Reljic et al, 2007;Marchal et al, 2008;Collin et al, 2009). All studies extracted DNA from blood/serum samples for further test except one (Singal et al, 2004), in which DNA was isolated from the archived paraffin blocks.…”

Association between MTHFR gene polymorphisms (C677T, A1298C) and genetic susceptibility to prostate cancer: a meta-analysis

“…Moreover, 2 studies were excluded because they did not provide the exact genotypes and the OR (95%CI) for the association between gene polymorphisms and prostate cancer risk could not be estimated (Zacho et al, 2011;Jackson et al, 2013). As a result, 21 case-control studies were included in our meta-analysis (Kimura et al, 2000;Heijmans et al, 2003;Cicek et al, 2004;Singal et al, 2004;Van Guelpen et al, 2006;Johansson et al, 2007;Reljic et al, 2007;Marchal et al, 2008;Stevens et al, 2008;Collin et al, 2009;Muslumanoglu et al, 2009;Cai et al, 2010;Safarinejad et al, 2010;Wu et al, 2010;Küçükhüseyin et al, 2011;Fard-Esfahani et al, 2012;Kobayashi et al, 2012;Mandal et al, 2012;de Vogel et al, 2013;López-Cortés et al, 2013;Ghasemi et al, 2014).…”