The analysis of subtle internal communications through mutation studies in periplasmic metal uptake protein CLas-ZnuA2

Saini, Gunjan; Sharma, Nidhi; Dalal, Vikram; Warghane, Ashish; Ghosh, Dilip K.; Kumar, Pravindra; Sharma, Ashwani

doi:10.1016/j.jsb.2018.08.013

Cited by 7 publications

(4 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Crystal structure showed that CLas-ZnuA2 binds both Zn 2+ and Mn 2+ with square pyramidal geometry, different from Mn-specific SBPs (tetrahedral geometry). The crystal structure studies of CLas-ZnuA2 (S38A and Y68F) protein demonstrate thatit is mutant in a metal-bound and metal-free states, confirming the subtle communication [ 149 ]. Binding studies of CLas-ZnuA2 by Surface Plasmon Resonance (SPR) revealed the low metal-binding affinity.…”

Section: Triangular Management Strategiesmentioning

confidence: 70%

Huanglongbing Pandemic: Current Challenges and Emerging Management Strategies

Ghosh

Kokane

Savita

et al. 2022

Plants

Self Cite

View full text Add to dashboard Cite

Huanglongbing (HLB, aka citrus greening), one of the most devastating diseases of citrus, has wreaked havoc on the global citrus industry in recent decades. The culprit behind such a gloomy scenario is the phloem-limited bacteria “Candidatus Liberibacter asiaticus” (CLas), which are transmitted via psyllid. To date, there are no effective long-termcommercialized control measures for HLB, making it increasingly difficult to prevent the disease spread. To combat HLB effectively, introduction of multipronged management strategies towards controlling CLas population within the phloem system is deemed necessary. This article presents a comprehensive review of up-to-date scientific information about HLB, including currently available management practices and unprecedented challenges associated with the disease control. Additionally, a triangular disease management approach has been introduced targeting pathogen, host, and vector. Pathogen-targeting approaches include (i) inhibition of important proteins of CLas, (ii) use of the most efficient antimicrobial or immunity-inducing compounds to suppress the growth of CLas, and (iii) use of tools to suppress or kill the CLas. Approaches for targeting the host include (i) improvement of the host immune system, (ii) effective use of transgenic variety to build the host’s resistance against CLas, and (iii) induction of systemic acquired resistance. Strategies for targeting the vector include (i) chemical and biological control and (ii) eradication of HLB-affected trees. Finally, a hypothetical model for integrated disease management has been discussed to mitigate the HLB pandemic.

show abstract

Section: Triangular Management Strategiesmentioning

confidence: 70%

Huanglongbing Pandemic: Current Challenges and Emerging Management Strategies

Ghosh

Kokane

Savita

et al. 2022

Plants

Self Cite

View full text Add to dashboard Cite

show abstract

“…To date, there are no protein structures for Ca . L. solanacearum reported and structures from Liberibacter species in general are limited (Jiang et al, 2014 ; Kumar, Dalal, et al, 2019 ; Kumar, Kesari, et al, 2019 ; Nan et al, 2020 ; Saini et al, 2018 ), which hinders our understanding of the biology of this genus. We studied the Ca .…”

Section: Discussionmentioning

confidence: 99%

A new lysine biosynthetic enzyme from a bacterial endosymbiont shaped by genetic drift and genome reduction

Gilkes,

Frampton,

Board

et al. 2024

Protein Science

View full text Add to dashboard Cite

The effect of population bottlenecks and genome reduction on enzyme function is poorly understood. Candidatus Liberibacter solanacearum is a bacterium with a reduced genome that is transmitted vertically to the egg of an infected psyllid—a population bottleneck that imposes genetic drift and is predicted to affect protein structure and function. Here, we define the function of Ca. L. solanacearum dihydrodipicolinate synthase (CLsoDHDPS), which catalyzes the committed branchpoint reaction in diaminopimelate and lysine biosynthesis. We demonstrate that CLsoDHDPS is expressed in Ca. L. solanacearum and expression is increased ~2‐fold in the insect host compared to in planta. CLsoDHDPS has decreased thermal stability and increased aggregation propensity, implying mutations have destabilized the enzyme but are compensated for through elevated chaperone expression and a stabilized oligomeric state. CLsoDHDPS uses a ternary‐complex kinetic mechanism, which is to date unique among DHDPS enzymes, has unusually low catalytic ability, but an unusually high substrate affinity. Structural studies demonstrate that the active site is more open, and the structure of CLsoDHDPS with both pyruvate and the substrate analogue succinic‐semialdehyde reveals that the product is both structurally and energetically different and therefore evolution has in this case fashioned a new enzyme. Our study suggests the effects of genome reduction and genetic drift on the function of essential enzymes and provides insights on bacteria‐host co‐evolutionary associations. We propose that bacteria with endosymbiotic lifestyles present a rich vein of interesting enzymes useful for understanding enzyme function and/or informing protein engineering efforts.

show abstract

“…As yet, there are no molecular structures for Ca. L. solanacearum proteins reported and structures from Liberibacter species in general are limited (Jiang et al 2014;Saini et al 2018;Kumar, Kesari, et al 2019;Kumar, Dalal, et al 2019;Nan et al 2020), which hinders our understanding of the biology of this genus. This investigation to express, purify and characterise the 'Candidatus Liberibacter solanacearum' lysine biosynthetic enzyme dihydrodipicolinate synthase, which is well studied in other bacterial systems, provides a unique opportunity to assess how evolution shapes enzymes in context of a reduced genome and a bottlenecked lifestyle.…”

Section: Discussionmentioning

confidence: 99%

Genetic drift and genome reduction in the plant pathogenCandidatusLiberibacter solanacearum shapes a new enzyme in lysine biosynthesis

Gilkes

Frampton

Board

et al. 2023

Preprint

View full text Add to dashboard Cite

The effect of population bottlenecks and genome reduction on enzyme function is poorly understood. 'Candidatus Liberibacter solanacearum' is a bacterium with a reduced genome that is transmitted vertically to the egg of an infected psyllid - a population bottleneck that imposes genetic drift and is predicted to affect protein structure and function. Here, we define the effects of genome reduction and genetic drift on the function of Ca. L. solanacearum dihydrodipicolinate synthase (CLsoDHDPS), which catalyses the committed branchpoint reaction in diaminopimelate and lysine biosynthesis. We demonstrate that CLsoDHDPS is expressed in Ca. L. solanacearum and expression is increased ~2-fold in the insect host compared to in planta. CLsoDHDPS has increased aggregation propensity, implying mutations have destabilised the enzyme but are compensated for through elevated chaperone expression and a stabilised oligomeric state. CLsoDHDPS uses a ternary-complex kinetic mechanism, which is unique among DHDPS enzymes, has unusually low catalytic ability, but an unusually high substrate affinity. Structural studies demonstrate that the active site is more open, and the structure of CLsoDHDPS with both pyruvate and the substrate analogue succinic-semialdehyde reveals that the product is both structurally and energetically different and therefore evolution has in this case fashioned a new enzyme. Our study reveals the effects of genome reduction and genetic drift on the function of essential enzymes and provides insights on bacteria-host co-evolutionary association. We suggest that bacteria with endosymbiotic lifestyles present a rich vein of interesting enzymes useful for understanding enzyme function and/or informing protein engineering efforts.

show abstract

The analysis of subtle internal communications through mutation studies in periplasmic metal uptake protein CLas-ZnuA2

Cited by 7 publications

References 22 publications

Huanglongbing Pandemic: Current Challenges and Emerging Management Strategies

Huanglongbing Pandemic: Current Challenges and Emerging Management Strategies

A new lysine biosynthetic enzyme from a bacterial endosymbiont shaped by genetic drift and genome reduction

Genetic drift and genome reduction in the plant pathogenCandidatusLiberibacter solanacearum shapes a new enzyme in lysine biosynthesis

Contact Info

Product

Resources

About