The Analysis of Metallothionein Immunoreactivity in Stromal Fibroblasts and Macrophages in Cases of Uterine Cervical Carcinoma With Respect to Both the Local and Distant Spread of the Disease

Walentowicz-Sadlecka, Małgorzata; Koper, Anna; Gałązka, Krystyna; Koper, Krzysztof; Basta, Paweł; Mach, Paweł; Skręt-Magierło, Joanna; Dutsch-Wicherek, Magdalena; Sikora, Jerzy; Grabiec, Marek; Kaźmierczak, Wojciech; Wicherek, Lukasz

doi:10.1111/aji.12120

Cited by 13 publications

(14 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In the present study, MT immunoreactivity was also found to be expressed by the stromal fibroblasts. This correlates with the pattern of MT expression in other histological tumour types, such as adenocarcinomas and squamous cell carcinomas [22][23][24][25]. Stroma is tissue that undergoes intensive remodelling during tumour development, which is also induced by factors secreted from its own tumour cells [40].…”

Section: Discussionsupporting

confidence: 69%

“…In our previous studies, we demonstrated and analysed the involvement of the tumour microenvironment in the development of various types of malignant epithelial neoplasms. The microenvironment was associated with its own remodelling and the acquisition of a suppressive tumour microenvironment [22][23][24][25]. Additionally, we demonstrated the presence of tumour microenvironment remodelling with RCAS1 expression and RCAS1-positive macrophages in the tumour microenvironment of palatine tonsil DLBCL.…”

Section: Introductionmentioning

confidence: 59%

See 1 more Smart Citation

Metallothionein immunoreactivity profile in B-cell lymphomas of the palatine tonsils

Dutsch-Wicherek

Tomaszewska

Lazar

et al. 2017

Medical Research Journal

Self Cite

View full text Add to dashboard Cite

Section: Discussionsupporting

confidence: 69%

Section: Introductionmentioning

confidence: 59%

Metallothionein immunoreactivity profile in B-cell lymphomas of the palatine tonsils

Dutsch-Wicherek

Tomaszewska

Lazar

et al. 2017

Medical Research Journal

Self Cite

View full text Add to dashboard Cite

“…This fact can also be supported by the finding that fibroblasts exhibited high vimentin and MT immunoreactivity levels in the tumour microenvironment [19], which could be reason for the higher expression of MT in the tumour-adjacent tissues. Furthermore, the rate of MT protein expression in macrophages and fibroblasts in the tumour microenvironment appears to be an indicator of microenvironment remodelling associated with the local progression of cancer [25]. Higher MT expression was detected in the primary tumour itself than in its derived lymph node metastases [26].…”

Section: Discussionmentioning

confidence: 99%

Prognostic significance of the tumour-adjacent tissue in head and neck cancers

et al. 2015

View full text Add to dashboard Cite

Even with significant advances in operative skills and adjuvant therapies, the overall survival of patients suffering with head and neck squamous cancers (HNSCC) is unsatisfactory. Accordingly, no clinically useful prognostic biomarkers have been found yet for HNSCC. Many studies analysed the expression of potential markers in tumour tissues compared to adjacent tissues. Nevertheless, due to the sharing of the same microenvironment, adjacent tissues show molecular similarity to tumour tissues. Thus, gene expression patterns of 94 HNSCC tumorous tissues were compared with 31 adjacent tissues and with 10 tonsillectomy specimens of non-cancer individuals. The genes analysed at RNA level using quantitative RT-PCR and correlated with clinico-pathological conditions were as follows: EGF, EGFR, MKI67, BCL2, BAX, FOS, JUN, TP53, VEGF, FLT1, MMP2, MMP9, MT1A and MT2A. The elevated MT2A, BAX, EGF and JUN expression was associated with the influence of tumour cells on the rearrangement of healthy tissues, as well as a significant shift in the BAX/BCL2 ratio. Our investigation also indicated that adjacent tissues play an important role in cancerogenesis by releasing several tumour-supporting factors such as EGF. A gradual increase in the metallothionein expression, from the lowest one in tonsillectomy samples to the highest ones in tumour samples, suggests that MT expression might be tissue reaction to the presence of tumour cells. The results of this study confirmed the significance of metallothionein in tumori-genesis and gave evidences for its use as a potential HNSCC biomarker. Furthermore, this study highlighted the importance of histologically normal tumour-adjacent tissue in prediction of HNSCC progress.

show abstract

“…Treg cells are not only responsible for the evasion of cancer cells from immune surveillance, but also cooperate with other immune cells, including TAMs, which are predominantly from M2 subgroups [29,30]. These subgroups are responsible for the secretion of TGF-beta and Il-10 that support the differentiation of Treg cells [31].…”

Section: Discussionmentioning

confidence: 99%

Analysis of the treg cell population in the peripheral blood of ovarian cancer patients in relation to the long-term outcomes

Dutsch-Wicherek

Szubert²,

Dziobek

et al. 2019

Ginekol Pol

Self Cite

View full text Add to dashboard Cite

Objectives: There is growing evidence that Treg cell infiltration into the cancer nest is associated with poor prognosis. However, the Treg cell population in the peripheral blood may change when a different type of anticancer therapy is applied. Since Treg cells may support tumor growth by enhancing the suppressive profile of the cancer microenvironment, the assessment of Treg cells can bring to light important information regarding prognosis. Thus we decided to analyze the Treg cell population in the peripheral blood in relation to long-term outcomes in the group of patients with ovarian cancer. Material and methods:The 80 patients included in the study were treated surgically followed by chemiotherapy for ovarian cancer between October 2010 through May 2011.The peripheral blood samples from the patients were collected directly prior to chemotherapy. Information on any patients who died was retrieved from the database of the Cuiavia-Pomerania Regional Office of the National Health System of Poland. CD4+CD25+FOXP3+ lymphocytes T were assed by flow cytometry. We have analyzed the long term outcomes of treatment regarding to the level of Treg cells in peripheral blood. Results:We found that patients with serous adenocarcinomas had significantly higher Treg levels compared to those patients with non-serous types. Patients who had a higher percentage of Treg cells within the CD4+ cell population prior to the beginning of the treatment had worse long-term outcomes from the applied therapy. Conclusions:The assessment of Treg levels prior to the start of chemotherapy is clinically useful and may predict overall survival in ovarian cancer patients.

show abstract

The Analysis of Metallothionein Immunoreactivity in Stromal Fibroblasts and Macrophages in Cases of Uterine Cervical Carcinoma With Respect to Both the Local and Distant Spread of the Disease

Cited by 13 publications

References 35 publications

Metallothionein immunoreactivity profile in B-cell lymphomas of the palatine tonsils

Metallothionein immunoreactivity profile in B-cell lymphomas of the palatine tonsils

Prognostic significance of the tumour-adjacent tissue in head and neck cancers

Analysis of the treg cell population in the peripheral blood of ovarian cancer patients in relation to the long-term outcomes

Contact Info

Product

Resources

About