The analysis of magnesium transport system from external solution to xylem in rice root

Tanoi, Keitaro; Saito, Takayuki; Iwata, Naoyuki; Kobayashi, Natsuko I.; Nakanishi, Teruyuki

doi:10.1080/00380768.2011.576397

Cited by 28 publications

(14 citation statements)

References 23 publications

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“…Rice seedlings ( Oryza sativa L. cv. Nipponbare ) were grown in half‐strength Kimura B nutrient solution (pH 5.6) in a growth chamber under a day/night cycle of 16/8 h at 30°C with 70% humidity (Tanoi et al ). The composition of the half‐strength Kimura B nutrient solution (µ M ) was (NH 4 ) 2 SO 4 (180), MgSO 4 (270), KNO 3 (91), Ca(NO 3 ) 2 (180), KH 2 PO 4 (91), K 2 SO 4 (46), Fe‐citrate (45), MnCl 2 (6.7), H 3 BO 3 (9), ZnSO 4 (0.15), CuSO 4 (0.16) and (NH 4 ) 6 Mo 7 O 24 (0.015).…”

Section: Methodsmentioning

confidence: 99%

Leaf senescence in rice due to magnesium deficiency mediated defect in transpiration rate before sugar accumulation and chlorosis

et al. 2012

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Magnesium (Mg) is an essential macronutrient supporting various functions, including photosynthesis. However, the specific physiological responses to Mg deficiency remain elusive. In this study, 2-week-old rice seedlings (Oryza sativa. cv. Nipponbare) with three expanded leaves (L2-L4) were transferred to Mg-free nutrient solution for 8 days. In the absence of Mg, on day 8, L5 and L6 were completely developed, while L7 just emerged. We also studied several mineral deficiencies to identify specific responses to Mg deficiency. Each leaf was analyzed in terms of chlorophyll, starch, anthocyanin and carbohydrate metabolites, and only absence of Mg was found to cause irreversible senescence of L5. Resupply of Mg at various time points confirmed that the borderline of L5 death was between days 6 and 7 of Mg deficiency treatment. Decrease in chlorophyll concentration and starch accumulation occurred simultaneously in L5 and L6 blades on day 8. However, nutrient transport drastically decreased in L5 as early as day 6. These data suggest that the predominant response to Mg deficiency is a defect in transpiration flow. Furthermore, changes in myo-inositol and citrate concentrations were detected only in L5 when transpiration decreased, suggesting that they may constitute new biological markers of Mg deficiency.

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Section: Methodsmentioning

confidence: 99%

Leaf senescence in rice due to magnesium deficiency mediated defect in transpiration rate before sugar accumulation and chlorosis

et al. 2012

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“…It is unknown whether they behavior similarly in planta. The K m value for wholeroot Mg uptake was estimated to be 20 mM (Tanoi et al, 2011), while that for OsMGT1 was 30 mM (Fig. 7).…”

Section: Osmgt1 Is a Transporter For Mg Uptakementioning

confidence: 99%

Up-Regulation of a Magnesium Transporter GeneOsMGT1Is Required for Conferring Aluminum Tolerance in Rice

Yamaji²,

et al. 2012

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Magnesium (Mg)-mediated alleviation of aluminum (Al) toxicity has been observed in a number of plant species, but the mechanisms underlying the alleviation are still poorly understood. When a putative rice (Oryza sativa) Mg transporter gene, Oryza sativa MAGNESIUM TRANSPORTER1 (OsMGT1), was knocked out, the tolerance to Al, but not to cadmium and lanthanum, was decreased. However, this inhibition could be rescued by addition of 10 mM Mg, but not by the same concentration of barium or strontium. OsMGT1 was expressed in both the roots and shoots in the absence of Al, but the expression only in the roots was rapidly up-regulated by Al. Furthermore, the expression did not respond to low pH and other metals including cadmium and lanthanum, and was regulated by an Al-responsive transcription factor, AL RESISTANCE TRANSCRIPTION FACTOR1. An investigation of subcellular localization showed that OsMGT1 was localized to the plasma membrane. A short-term (30 min) uptake experiment with stable isotope 25 Mg showed that knockout of OsMGT1 resulted in decreased Mg uptake, but that the uptake in the wild type was enhanced by Al. Mg concentration in the cell sap of the root tips was also increased in the wild-type rice, but not in the knockout lines in the presence of Al. A microarray analysis showed that transcripts of genes related to stress were more up-and down-regulated in the knockout lines. Taken together, our results indicate that OsMGT1 is a transporter for Mg uptake in the roots and that up-regulation of this gene is required for conferring Al tolerance in rice by increasing Mg concentration in the cell.

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“…Wild‐type seeds of Oriza sativa (L. cv Nipponbare) were cultivated on a KimuraB/2 medium (Tanoi et al ., ).…”

Section: Methodsmentioning

confidence: 97%

A chemical genetic strategy identify the PHOSTIN, a synthetic molecule that triggers phosphate starvation responses in Arabidopsis thaliana

et al. 2015

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Summary Plants display numerous strategies to cope with phosphate (Pi)‐deficiency. Despite multiple genetic studies, the molecular mechanisms of low‐Pi‐signalling remain unknown. To validate the interest of chemical genetics to investigate this pathway we discovered and analysed the effects of PHOSTIN (PSN), a drug mimicking Pi‐starvation in Arabidopsis.We assessed the effects of PSN and structural analogues on the induction of Pi‐deficiency responses in mutants and wild‐type and followed their accumulation in plants organs by high pressure liquid chromotography (HPLC) or mass‐spectrophotometry.We show that PSN is cleaved in the growth medium, releasing its active motif (PSN11), which accumulates in plants roots. Despite the overaccumulation of Pi in the roots of treated plants, PSN11 elicits both local and systemic Pi‐starvation effects. Nevertheless, albeit that the transcriptional activation of low‐Pi genes by PSN11 is lost in the phr1;phl1 double mutant, neither PHO1 nor PHO2 are required for PSN11 effects.The range of local and systemic responses to Pi‐starvation elicited, and their dependence on the PHR1/PHL1 function suggests that PSN11 affects an important and early step of Pi‐starvation signalling. Its independence from PHO1 and PHO2 suggest the existence of unknown pathway(s), showing the usefulness of PSN and chemical genetics to bring new elements to this field.

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The analysis of magnesium transport system from external solution to xylem in rice root

Cited by 28 publications

References 23 publications

Leaf senescence in rice due to magnesium deficiency mediated defect in transpiration rate before sugar accumulation and chlorosis

Leaf senescence in rice due to magnesium deficiency mediated defect in transpiration rate before sugar accumulation and chlorosis

Up-Regulation of a Magnesium Transporter GeneOsMGT1Is Required for Conferring Aluminum Tolerance in Rice

A chemical genetic strategy identify the PHOSTIN, a synthetic molecule that triggers phosphate starvation responses in Arabidopsis thaliana

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