The analysis of correlation between IL-12 gene expression and hepatitis B virus in the affected patients

Abstract

Hepatitis B viral (HBV) infection, which is one of the global public health concerns, is among the most common causes of cirrhosis and hepatocellular carcinoma. It was proposed that cytokine-mediated immunity plays a critical role in determining the outcomes of hepatitis B virus infection. Interleukin 12 (IL-12) is a heterodimeric cytokine that shows a broad range of immunoregulatory properties during immune responses and combats host invading pathogens. The main purpose of this study was to investigate the possible association between expression levels of IL-12 gene with HBV infection in patients with HBV infection. This clinical study was performed on 30 HBV patients and 30 healthy controls. SYBR Green Realtime PCR was performed to examine the expression level of IL-12 gene in HBV patients. Then, the rate of expression was calculated using the Livak (2 ÀDDC T ) method. DC T of samples in the two groups were compared using t test method. PCR was also used for HBV-DNA evidence. The results of our study demonstrated that the difference in mean of IL-12 gene expression between healthy subjects and HBV patients is statistically significant.

Interleukin-35 Level Is Reduced in Patients with Chronic Hepatitis B Virus Infection

“…In addition, serum IL-27 is elevated in HBV patients 22 and IL-27 treatment could inhibit HBV replication 23 . It is also reported that IL-12 expression in HBV patients is significantly different from the healthy subjects 24 . However, the relationship between IL-35 and HBV replication has not been reported yet.…”