The analysis of chromatin organisation allows selection of mouse antral oocytes competent for development to blastocyst

Zuccotti, Maurizio; Ponce, Rubén Hugo; Boiani, Michele; Guizzardi, Stefano; Govoni, Paolo; Scandroglio, R; Garagna, Silvia; Redi, Carlo Alberto

doi:10.1017/s0967199402002101

Cited by 124 publications

(102 citation statements)

References 44 publications

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“…The mammalian oocyte nucleus or germinal vesicle (GV) is modified at the level of chromatin patterning during oogenesis and alterations in the location and extent of heterochromatization have been related to the oocyte metabolic properties and competencies [1][2][3], (reviewed in [4][5][6][7]). During meiotic arrest at Prophase I, the oocyte's chromosomes lose their individuality and form a loose chromatin mass, which in turn undergoes profound and dynamic rearrangements within the GV concentrating in a small area of the nucleoplasm before the meiotic resumption.…”

Section: Introductionmentioning

confidence: 99%

Role of gap junction-mediated communications in regulating large-scale chromatin configuration remodeling and embryonic developmental competence acquisition in fully grown bovine oocyte

Lodde

Franciosi

Tessaro

et al. 2013

J Assist Reprod Genet

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Purpose This study was aimed to test the hypothesis that gap junction mediated communications (GJC) are required to allow the progressive chromatin configuration remodeling (from GV1 to GV3) process to occur in fully grown oocytes in order to gain the final step of developmental competence acquisition, and that a premature disruption of GJC can alter this process. Methods Bovine cumulus-oocytes complexes collected from medium antral follicles were cultured for 2, 4, 6 and 8 h in the presence of 10 −4 IU/ml of r-hFSH and with 2 mM of the nonselective PDE inhibitor 3-isobutyl-1-methyl-xanthine (IBMX) to prevent meiotic resumption. GJC functionality and chromatin configuration were monitored during the culture period. After meiotic arrest, the developmental capability of oocytes was assessed after IVM and IVF. Results IBMX was effective in significantly sustaining GJC up to 6 h and maintaining meiotic arrest, when compared to control group. Moreover, the percentage of oocytes with less condensed chromatin (GV1) decreased within 4 h of culture, while the proportion of GV2 oocytes gradually increased up to 6 h.Interestingly, a decline in the proportion of GV2 oocytes and an increase in the proportion of GV3 oocytes were observed after 6 h of culture, when the major drop of GJC occurred. On the contrary, when GJC were uncoupled by adding 3 mM of 1-heptanol or through cumulus cells removal, chromatin condensation occurred rapidly throughout the culture period, more promptly in denuded oocytes. Moreover, the maintenance of GJC during meiotic arrest was accompanied by a significant increase of developmental competence compared to the control, as indicated by a higher percentage of hatched blastocysts and blastocyst cell number. Conclusions Altogether, our data indicate that both paracrine and junctional mechanisms are involved in modulating largescale chromatin structure during the final phase of oocyte differentiation.

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Section: Introductionmentioning

confidence: 99%

Role of gap junction-mediated communications in regulating large-scale chromatin configuration remodeling and embryonic developmental competence acquisition in fully grown bovine oocyte

Lodde

Franciosi

Tessaro

et al. 2013

J Assist Reprod Genet

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“…Accordingly, among the oo-cytes isolated from antral follicles, often referred to as "fully grown oocytes", there are still oocytes with NSN chromatin as well as oocytes having more developmentally advanced SN chromatin. Importantly, NSN oocytes do not develop beyond the 2-cell stage upon meiotic maturation and fertilization in vitro, in contrast to SN ones which are able to complete development (ZUCCOTTI et al 1998;ZUCCOTTI et al 2002). Thus, the SN type chromatin configuration may be a convenient marker of oocyte quality in those ART procedures in which prophase oocytes are used.…”

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confidence: 99%

Prediction of Developmentally Competent Chromatin Conformation in Mouse Antral Oocytes

Daszkiewicz¹,

Szymoniak²,

Gąsior³

et al. 2016

folia biol (krakow)

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DASZKIEWICZ R., SZYMONIAK M., G¥SIOR £., POLAÑSKI Z. 2016. Prediction of developmentally competent chromatin conformation in mouse antral oocytes. Folia Biologica (Kraków) 64: 59-65.Mouse prophase oocytes isolated from antral follicles may possess two alternative types of chromatin configuration: NSN configuration represents more dispersed chromatin and is characteristic mainly for growing oocytes whereas SN configuration, attained upon oocyte growth, comprises more condensed chromatin with a significant fraction concentrated around the nucleolus. Importantly, fully grown oocytes isolated from antral follicles represent a non-homogenous population in which some oocytes posses NSN-type and others SN-type of chromatin conformation. From these two, only oocytes with SN configuration are able to complete full development upon fertilization. We show that among mouse oocytes isolated from antral follicles, those surrounded by cumulus cells were larger and more frequently possessed SN chromatin than oocytes lacking the complete cumulus cell layer. Females primed with PMSG gave a higher number of oocytes with a complete layer of cumulus cells and the frequency of oocytes with SN chromatin was also elevated. Within the whole population of isolated antral oocytes, we observed subtle variation in size which allowed fractionation of oocytes under a stereomicroscope into groups representing oocytes of slightly different sizes. The occurrence of SN chromatin configuration was highly dependent on the oocyte size and its frequency increased gradually in subsequent size groups reaching 95-100% in the group representing the largest oocytes. These findings demonstrate that the subtle differences in the size of antral oocytes allow prediction of the status of their chromatin, thus providing a simple, fast, non-invasive and non-expensive way to select good quality oocytes for ART purposes in mammals.

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“…As for the mice treated with PMSG, female mice (3 weeks) were injected i.p. with 7.5 units PMSG (Teikokuzouki, Tokyo, Japan) diluted in 0.2 ml 10 mM phosphate-buffered saline (PBS), following the PMSG treatment protocol to synchronize the ovarian cycles in the mice (Mitsunari et al 1999, Zuccotti et al 2002. For the PMSG-treated male mice, we also applied the same PMSG treatment protocol (i.e.…”

Section: Animalsmentioning

confidence: 99%

A close correlation in the expression patterns of Af-6 and Usp9x in Sertoli and granulosa cells of mouse testis and ovary

Satô¹,

Kanai²,

Noma³

et al. 2004

Reproduction

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Usp9x, an X-linked deubiquitylating enzyme, is stage dependently expressed in the supporting cells (i.e. Sertoli cells and granulosa cells) and germ cells during mouse gametogenesis. Af-6, a cell junction protein, has been identified as a substrate of Usp9x, suggesting a possible association between Usp9x and Af-6 in spermatogenesis and oogenesis. In this study, we examined the expression pattern of Af-6 and Usp9x and their intracellular localization in testes and ovaries of mice treated with or without pregnant mare serum gonadotropin (PMSG), an FSH-like hormone. In both testes and ovaries, Af-6 expression was predominantly observed in supporting cells, as well as in steroidogenic cells, but not in any germ cells. In Sertoli cells, Af-6 was continuously expressed throughout postnatal and adult stages, where both Af-6 and Usp9x were enriched at the sites of Sertoli-Sertoli and Sertoli -spermatid junctions especially at stages XI -VI. In the granulosa cells, Af-6, as well as Usp9x, was highly expressed in primordial and primary follicles, but its expression rapidly decreased after the late-secondary follicle stage. Interestingly, in PMSG-treated mice, the expression levels of Af-6 and Usp9x were synchronously enhanced, slightly in Sertoli cells and strongly in granulosa cells of the late-secondary and Graafian follicles. Such closely correlated expression patterns between Af-6 and Usp9x clearly suggest that Af-6 may be deubiquitylated by Usp9x in both Sertoli and granulosa cells. It further suggests that the post-translational regulation of Af-6 by Usp9x may be one potential pathway to control the cell adhesion dynamics in mammalian gametogenesis.

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The analysis of chromatin organisation allows selection of mouse antral oocytes competent for development to blastocyst

Cited by 124 publications

References 44 publications

Role of gap junction-mediated communications in regulating large-scale chromatin configuration remodeling and embryonic developmental competence acquisition in fully grown bovine oocyte

Role of gap junction-mediated communications in regulating large-scale chromatin configuration remodeling and embryonic developmental competence acquisition in fully grown bovine oocyte

Prediction of Developmentally Competent Chromatin Conformation in Mouse Antral Oocytes

A close correlation in the expression patterns of Af-6 and Usp9x in Sertoli and granulosa cells of mouse testis and ovary

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