The Analysis of Cell Lineages in the Postimplantation Mammalian Embryo

Beddington, Rosa

doi:10.1007/978-3-642-49323-2_2

Cited by 2 publications

(2 citation statements)

References 33 publications

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“…Histochemical staining for ␤-gal activity was performed according to Beddington and Lawson (1990). Stained embryos were embedded in methacrylate (Technovit 8100; HeraeusKulzer, Werheim, Germany) and sectioned at 8 µm.…”

Section: Histologymentioning

confidence: 99%

Dmdmdx-βgeo: A new allele for the mouse dystrophin gene

Wertz

Füchtbauer

1998

Dev. Dyn.

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During a gene trap screen, an insertion of the gene trap vector into the dystrophin gene, creating a new allele for the Dmd gene, has been discovered. Because the ROSAβgeo vector was used, the new allele is called Dmdmdx‐βgeo. The insertion occurred 3′ of exon 63 of the dystrophin gene, resulting in a mutation that affects all presently known dystrophin isoforms. In contrast to spontaneous or ENU‐induced alleles, Dmdmdx‐βgeo can be used to follow dystrophin expression by staining for β‐galactosidase activity. The high sensitivity of this method revealed additional and earlier expression of dystrophin during embryogenesis than that seen previously with other methods. Dystrophin promoters are active predominantly in the dermamyotome, limb buds, telencephalon, floor plate, eye, liver, pancreas anlagen, and cardiovascular system. Adult Dmdmdx‐βgeo mice show reporter gene expression in brain, eye, liver, pancreas, and lung. In skeletal and heart muscle, β‐galactosidase activity is not detectable, confirming Western blot data that indicate the absence of the mutant full‐length protein in these tissues. Hemizygous Dmdmdx‐βgeo mice show muscular dystrophy with degenerating muscle fibers, cellular infiltration, and regenerated muscle fibers that have centrally located nuclei. Some mutant animals develop a dilated esophagus, probably due to constriction by the hypertrophic crura of the diaphragm. Dev. Dyn. 1998;212:229–241. © 1998 Wiley‐Liss, Inc.

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Section: Histologymentioning

confidence: 99%

Dmdmdx-βgeo: A new allele for the mouse dystrophin gene

Wertz

Füchtbauer

1998

Dev. Dyn.

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“…It has been established that the epiblast, a single epithelial sheet formed during gastrulation, gives rise to the germ layers: ectoderm, endoderm, and mesoderm. These ultimately give rise to all of the differentiated somatic cells of the mammalian body (for review see Beddington, 1981). The molecular mechanisms underlying these steps, their regulatory signals, as well as the genes that are expressed during the initiation of the germ layers have not been fully elucidated.…”

Section: Introductionmentioning

confidence: 99%

Claudin‐6: A novel tight junction molecule is developmentally regulated in mouse embryonic epithelium

Turksen

Troy

2001

Developmental Dynamics

118

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Embryonic stem (ES) cells differentiating into embryoid bodies (EBs) have been shown to mimic events of very early development and have become a convenient system in which to identify and study early epithelial specific genes. We describe here the primary structure of a mouse epithelial-specific tight junction gene and its expression patterns in differentiating ES cell-derived EBs in vitro. Sequencing The results indicate that Claudin-6 is one of the earliest molecules to be expressed in ES cells committed to the epithelial fate, and the onset of its expression coincides with the expression of the early epithelial marker, keratin 8 (K8). The initiation of expression of Claudin-6 in vitro is dependent upon plating density as well as serum components. In addition, it was found that Claudin-6 expression is inhibited by Noggin, the Bone Morphogenic Protein (BMP)-signalling pathway inhibitor, suggesting that BMPs may be involved in Claudin-6 expression and epithelialization. These studies establish Claudin-6 as a very early marker of epithelialization and provide evidence that the BMP signalling pathway may be one of the ways that its expression is regulated. These studies also support the power of in vitro ES cell technology to identify and screen novel molecules involved in the early epithelialization of the mouse embryo.

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The Analysis of Cell Lineages in the Postimplantation Mammalian Embryo

Cited by 2 publications

References 33 publications

Dmdmdx-βgeo: A new allele for the mouse dystrophin gene

Dmdmdx-βgeo: A new allele for the mouse dystrophin gene

Claudin‐6: A novel tight junction molecule is developmentally regulated in mouse embryonic epithelium

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