The analysis of association between Collal, VDR and CALCR genes and development of osteoporosis

Abstract

The allele rates of VDR, Collal and CALCR genes in 174 non-related individuals Northwest Russian population and in 70 patients with severe osteoporosis (SO) were investigated by PCR-RFLP method. The frequency of functionally abnormal allele t of VDR gene in a group of SO patients was Sl,4±5,9%, and it was significantly higher (p<0,001) than this one in population (32,6±4,9%). Analysis of Collal gene proved significant preponderance (p<0,0001) of functionally abnormal allele s in SO patients (50,0±5,9%) compared to its average frequency in population (17,5±4,9%). No significant differences (p>0,05) between frequencies of functionally abnormal T allele of CALCR gene in SO (84,4±4,9%) patients and its average frequency in population were recorded (73,8±3,9%). Thus, according to our data clear-cut association between functionally abnormal alleles of VDR and Collal genes and osteoporosis

Association Between Polymorphisms of VDR, COL1A1, and LCT Genes and Bone Mineral Density in Belarusian Women With Severe Postmenopausal Osteoporosis

“…The absence of association between the TaqI polymorphism of the VDR gene and severe postmenopausal osteoporosis in the belarusian women was very surprising because this polymorphism is very well studied, and its association with osteoporosis has been shown in many European populations (13,28), including Russian (29). Its mechanisms mostly involve the hormonal regulation of osteogenesis, inactivating the vitamin D receptor.…”

Role of TaqI-Genetic Polymorphism of Vitamin D Receptor Gene in Bone Metabolism in Children with Inflammatory Bowel Disease

“…Выделение ДНК про-водилось из периферической крови методом фе-нольно-хлороформной экстракции [26]. Для полимеразной цепной реакции использовали олиго-праймеры 5'-GATGATCCAGAAGCTAGCCGACCT-3', 5'-GCAACTCCTCATGGCTGAGGTCT-3' [4]. Про-дукты амплификации и рестрикции амплифициро-ванных фрагментов разделяли в 6-10 % неденатури-рующем полиакриламидном геле (ПААГ).…”

The genetic background of osteoporosis in cystic fibrosis: Association analysis with polymorphic markers in four candidate genes

“…CALCR/AluI RFLP was detected according to the conditions described [14].COL1A1-Sp1 binding site G/T dymorphism, originally described by Grant et al [18], was detected by using modified oligonucleotide primers and ApaI restriction enzyme according to Moskalenko et al [29]. Taq DNA polymerase was purchased from GIBCO-BRL, restriction endonucleases were purchased from New England Biolabs.…”