The analysis of artificial sweeteners and additives in beverages by HPLC: An undergraduate experiment

Bidlingmeyer, Brian A.; Schmitz, Stefan

doi:10.1021/ed068pa195

Cited by 15 publications

(14 citation statements)

References 2 publications

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“…To demonstrate the basic premise of using the PB-GDMS approach as a detector for a liquid chromatographic separation, a simple determination of organic components in a diet soft drink was performed . A mock solution of benzoic acid, caffeine, and aspartame was prepared in a 60:40 HPLC-grade methanol/water mixture.…”

Section: Methodsmentioning

confidence: 99%

Glow Discharge Ionization Source for Liquid Chromatography/Particle Beam Mass Spectrometry

and

Marcus

2000

Anal. Chem.

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A detailed evaluation of the analytical characteristics of a liquid chromatography/particle beam-glow discharge mass spectrometry (LC/PB-GDMS) system is described for applications in the area of inorganic (free metals in solution) and organic compound analysis. A highly efficient particle beam interface is used to introduce analyte species into a glow discharge source for subsequent vaporization and ionization. The GD operating current and gas pressure were optimized, with the general responses found to be similar to those obtained previously for particulate matter analysis by this PB-GDMS system. The mass spectra obtained for inorganic species are comprised exclusively of the respective elements' isotopic patterns, with no evidence of appreciable oxide species formation. Organic species introduced into the discharge through the particle beam interface yield mass spectra that are virtually identical to those from standard electron impact (70 eV) ionization. An analytical response curve for caffeine, using 200-microL (H2O/MeOH) injection volumes, showed less than 5% RSD for replicate injections over a concentration range of 10-500 ppm, with a detection limit of 13 ppb (2.7 ng) obtained for the caffeine molecular ion. Similarly, detection limits for Fe, Ag, and Cs ranged from 5.8 to 6.1 ppb (approximately 1 ng, each) in the injected volume. As an example of the feasibility of the PB-GDMS system as a detector for liquid chromatography, the separation and identification of the organic constituents in diet soda was performed.

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Section: Methodsmentioning

confidence: 99%

Glow Discharge Ionization Source for Liquid Chromatography/Particle Beam Mass Spectrometry

and

Marcus

2000

Anal. Chem.

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“…Several interesting applications of high-performance liquid chromatography (HPLC) for the analysis of consumer products in undergraduate laboratories have appeared in this Journal. These include the analysis of vitamin A in infant formula (1); acetaminophen, caffeine, aspirin, salicylamide, phenacetin, and paracetamol in analgesic tablets (2)(3)(4); chlorophyll a, chlorophyll b, and β-carotene in collard greens (5); aspartame, caffeine, saccharin, and benzoic acid in colas and other beverages (6)(7)(8)(9); coumarin in vanilla extracts (10,11); theophylline, ephedrine, and phenobarbital in asthma medication (12); riboflavin in milk and cheese (13); sugars in milk, colas, and beer (14); UV absorbers in sunscreens (15), and enantiomers of beta-blockers (16 ). An additional application of HPLC that is suitable for undergraduate laboratories is the quantitation of the components of hot-pepper sauces responsible for the perceived "heat".…”

mentioning

confidence: 99%

Pungency Quantitation of Hot Pepper Sauces Using HPLC

Betts

1999

J. Chem. Educ.

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The format of the laboratory described here allows students to collectively develop an HPLC method for the quantitation of the two predominant capsaicinoids (capsaicin and dihydrocapsaicin) in hot-pepper products. Each small group of students investigated one of the following aspects of the method: detector wavelength, mobile-phase composition, extraction of capsaicinoids, calibration, and quantitation. The format of the lab forced students to communicate and cooperate to develop this method.

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“…Two common undergraduate laboratory experiments involving HPLC are the analysis of caffeine in various beverage samples − and the characterization of aspirin tablets. − Here, example separations of these two common applications were obtained using the portable capillary LC instrument and compared to a standard benchtop HPLC instrument (Figure ). To maximize the number of students that can gain hands-on experience with an instrument during a single laboratory period (165 min), Rowan University has typically utilized a single-point calibration approach for the quantitation of caffeine in diet soda as part of its second-year undergraduate analytical chemistry laboratory course.…”

Section: Educational Applications Of Portable Capillary Lcmentioning

confidence: 99%

Use of Portable Capillary Liquid Chromatography for Common Educational Demonstrations Involving Separations

et al. 2021

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High-performance liquid chromatography is one of the primary techniques covered in the undergraduate analytical chemistry curriculum. This technology report describes the use of a portable capillary-scale instrument that can provide similar performance to a benchtop instrument but generates less solvent waste and enables operation in nonlaboratory settings. Comparisons between the two instrument types were made for single-standard calibration analysis of caffeine in diet soda and aspirin content in over-the-counter tablets, with relative percent differences between the standards and samples under 5% for both instrument types and both samples. The capability to use the instrument in lecture and outreach demonstration activities was also explored. Portable instruments can serve similar pedagogical purposes to traditional instruments as well as provide a platform to introduce discussions on green analytical chemistry based on differences in solvent waste generation and power consumption.

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The analysis of artificial sweeteners and additives in beverages by HPLC: An undergraduate experiment

Abstract: An undergraduate experiment analyzing artificial sweeteners and additives in beverages by HPLC.

Cited by 15 publications

References 2 publications

Glow Discharge Ionization Source for Liquid Chromatography/Particle Beam Mass Spectrometry

Glow Discharge Ionization Source for Liquid Chromatography/Particle Beam Mass Spectrometry

Pungency Quantitation of Hot Pepper Sauces Using HPLC

Use of Portable Capillary Liquid Chromatography for Common Educational Demonstrations Involving Separations

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