The analysis of 5′-mononucleotides in infant formulae by HPLC

Perrin, C.; Meyer, Liliane; Mujahid, Claudia; Blake, Christopher J

doi:10.1016/s0308-8146(01)00134-0

Cited by 35 publications

(25 citation statements)

References 11 publications

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“…Sample preparation was straightforward and protein precipitation was simply achieved by the addition of acid, as used by others (Ferreira et al, 2001;Indyk & Woollard, 2004;Perrin et al, 2001), in contrast to the more complex multi-enzyme treatment required for the determination of TPAN (Leach et al, 1995). With respect to chromatographic conditions, the Gemini column, a grafted polymer column designed to be stable over a large pH range, was also confirmed as being stable against phase collapse under 100% aqueous conditions.…”

Section: Discussionmentioning

confidence: 92%

“…Excellent resolution can be achieved because the ionic nature of the phosphate esters facilitates strong interactions with cationic ion-pair reagents at the appropriate pH (Brown, Robb, & Gelhart, 2002;Werner, 1993). Thus, IP-RPC has been employed to determine the nucleotide content in various mammalian milks and dairy products (Ferreira, Mendes, Gomes, Faria, & Ferreira, 2001;Mateo, Peters, & Stein, 2004;Oliveira, Ferreira, Mendes, & Ferreira, 1999;Perrin, Meyer, Mujahid, & Blake, 2001;Sugawara, Sato, Nakano, Idota, & Nakajima, 1995), in food ingredients (Fish, 1991) and in blood (Cichna et al, 2003).…”

Section: Introductionmentioning

confidence: 99%

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Development and application of a liquid chromatographic method for analysis of nucleotides and nucleosides in milk and infant formulas

Gill

Indyk

2007

International Dairy Journal

115

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Section: Discussionmentioning

confidence: 92%

Section: Introductionmentioning

confidence: 99%

Development and application of a liquid chromatographic method for analysis of nucleotides and nucleosides in milk and infant formulas

Gill

Indyk

2007

International Dairy Journal

115

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“…As a result of their ionic proper-ties, nucleotides strongly interact with cationic ion-pair reagents, if certain requirements are fulfilled, e.g. convenient pH [18][19][20][21]. The shortcomings of gradient elution in the presence of an ion-pair reagent, which cannot be neglected, include a prolonged equilibration time and a reduced column lifetime.…”

Section: Introductionmentioning

confidence: 99%

Analysis of nucleosides and monophosphate nucleotides from mushrooms with reversed‐phase HPLC

Ranogajec

Beluhan

Šmit

2010

J of Separation Science

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The retention characteristics of five stationary phases were tested by using a selection of 5'-mononucleotides and nucleosides with the aim to develop a simple, rapid and sensitive reversed-phase liquid chromatography method without ion-pair reagent usage. The method was optimized by changes in temperature, pH and ionic strength on a column showing a superior performance. The mobile phase consisted of a mixture of 0.05 M phosphate buffer and methanol, delivered at a flow rate of 0.4 mL/min and based on a gradient program. UV detection was used at a 254 nm wavelength. The method was validated for a quantitative analysis of 5'-mononucleotides and nucleosides in wild edible mushrooms. For all nucleosides and nucleotides, the LOD and LOQ were less than 0.02 and 0.07 microg/mL, respectively. Validation parameters yielded recovery rates between 68.6 and 98.2%, with a precision expressed as a relative standard deviation of 7.6-15.3%. The content of 5'-mononucleotides and nucleosides was determined for 10 samples of wild edible mushrooms found in Croatia and, accordingly, the equivalent umami concentrations were calculated.

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“…Nucleotides, such as disodium uridine monophosphate (Na 2 UMP) (Fig. 1c), are organic orthophosphates, which naturally occur in human milk at low concentrations and are added to baby food [19]. To our knowledge, no information is available about the interaction of Na 2 UMP with casein micelles.…”

Section: Introductionmentioning

confidence: 99%

Calcium-binding capacity of organic and inorganic ortho- and polyphosphates

Kort

Minor

Snoeren

et al. 2009

Dairy Sci. Technol.

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-The aim of this research was to determine the calcium-binding capacity of inorganic and organic ortho-and polyphosphates. This calcium-binding capacity can be used to influence the stability of, for example, casein micelles in dairy systems. Four phosphates were selected: disodium uridine monophosphate (Na 2 UMP, organic orthophosphate), disodium hydrogen phosphate (Na 2 HPO 4 , inorganic orthophosphate), sodium phytate (SP, organic polyphosphate), and sodium hexametaphosphate (SHMP, inorganic polyphosphate). Concentrations of up to 100 mmol·L −1 phosphate were added to a 50 mmol·L −1 CaCl 2 solution. The samples were prepared at pH 8.0 and were analyzed before and after sterilization for calcium-ion activity, conductivity, pH, sediment, and turbidity. Both SHMP and SP are strong chelators, as calcium ions bind to these phosphates in the ratio of 3:1 and 6:1, respectively. Calcium ions also strongly bind to Na 2 HPO 4 , but in a ratio of 3:2 with insoluble Ca 3 (PO 4 ) 2 complexes as result. The equilibrium position of Na 2 UMP is not strong towards the chelated complex, and significant levels of free calcium and free phosphate can exist. An equilibrium constant of 0.29 ± 0.08 L·mol −1 was determined for calcium uridine monophosphate (CaUMP) complexes. Both calculation of the equilibrium constant and analysis on the CaUMP precipitate confirmed a reactivity of 1:1 between calcium and Na 2 UMP. The CaUMP complexes are well soluble at ambient temperature, and insoluble complexes appear after sterilization, because the solubility of CaUMP decreases during heating. Finally, we concluded that the structure of phosphate molecules determines their calcium-binding capacity rather than organic or inorganic origin of phosphates.orthophosphate / polyphosphate / organic phosphate / inorganic phosphate / calcium 摘要 -有机和无机正磷酸盐和多磷酸盐对钙的结合能力○ 本文测定了无机和有机正磷酸和多磷酸盐对钙的结合能力○ 这种结合能力影响到乳体系中酪蛋白酪胶束的稳定○ 四种磷酸盐分别为 :尿苷磷酸二钠 (Na 2 UMP，有机正磷酸盐)，磷酸氢二钠 (Na 2 HPO 4 ，无机正磷酸盐)，肌醇六磷酸钠 (SP，有机多磷酸盐)和六偏磷酸钠 (SHMP，无机多磷酸盐)○ 100 mmol·L −1 的磷酸盐添加到 50 mmol·L −1 的 CaCl 2 溶液中○ 分析了杀菌前后样品在 pH 8.0 的钙离子活度、电导率、pH 值、沉淀值和浊度○ SHMP 和 SP 都是强螯合剂，钙离子与这些磷酸盐螯合的比分别为 3:1 和 6:1○ 同时，钙离子与 Na 2 HPO 4 结合能力较强，产生了 3:2 的不溶性 Ca 3 (PO 4 ) 2 混合物○ 平衡状态下的 Na 2 UMP 不能与钙形成稳定的螯合物，存

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The analysis of 5′-mononucleotides in infant formulae by HPLC

Cited by 35 publications

References 11 publications

Development and application of a liquid chromatographic method for analysis of nucleotides and nucleosides in milk and infant formulas

Development and application of a liquid chromatographic method for analysis of nucleotides and nucleosides in milk and infant formulas

Analysis of nucleosides and monophosphate nucleotides from mushrooms with reversed‐phase HPLC

Calcium-binding capacity of organic and inorganic ortho- and polyphosphates

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