The anabolic androgenic steroid nandrolone decanoate affects mRNA expression of dopaminergic but not serotonergic receptors

Birgner, Carolina; Kindlundh-Högberg, Anna M.S.; Alsiö, Johan; Lindblom, Jessica; Schiöth, Helgi B.; Bergström, Lena

doi:10.1016/j.brainres.2008.09.003

Cited by 27 publications

(18 citation statements)

References 59 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In addition, pharmacological manipulations that enhance dopaminergic activity facilitate male sexual behavior [reviewed in 37]. AAS treatment of adult mice and rats increases dopamine synthesis and receptors in the nucleus accumbens [38-42], although a single subcutaneous injection of T does not affect dopamine levels in the nucleus accumbens of Syrian hamsters [43]. In addition, nandrolone decanoate increases D1 receptor mRNA and decreases monoamine oxidase activity in the amygdala [42, 44].…”

Section: Discussionmentioning

confidence: 99%

“…AAS treatment of adult mice and rats increases dopamine synthesis and receptors in the nucleus accumbens [38-42], although a single subcutaneous injection of T does not affect dopamine levels in the nucleus accumbens of Syrian hamsters [43]. In addition, nandrolone decanoate increases D1 receptor mRNA and decreases monoamine oxidase activity in the amygdala [42, 44]. Both sexual satiety and testosterone are associated with increased dopamine activity within the medial preoptic area (MPOA) of rats [45], which receives projections from the amygdala [30, 35, 46]; however it is unknown if dopaminergic tone is affected by this AAS cocktail in the MPOA.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Anabolic steroids have long-lasting effects on male social behaviors

Salas-Ramirez

Montalto

Sisk

2010

Behavioural Brain Research

View full text Add to dashboard Cite

Anabolic androgenic steroids (AAS) use by adolescents is steadily increasing. Adolescence involves remodeling of steroid-sensitive neural circuits that mediate social behaviors, and previous studies using animal models document effects of AAS on male social behaviors. The present experiments tested whether AAS have persistent and more pronounced behavioral consequences when drug exposure occurs during adolescence as compared to exposure in adulthood. Male Syrian hamsters were injected daily for 14 days with either vehicle or an AAS cocktail containing testosterone cypionate (2 mg/kg), nandrolone decanoate (2 mg/kg), and boldenone undecylenate (1 mg/kg), either during adolescence (27-41 days of age) or adulthood (63-77 days of age). As adults, subjects were tested two or four weeks after the last injection for either sexual behavior with a receptive female or male-male agonistic behavior in a resident-intruder test. Compared with vehicle-treated males, AAStreated males, regardless of age of treatment, displayed fewer long intromissions and a significant increase in latency to the first long intromission, indicative of reduced potential to reach sexual satiety. Increased aggression was observed in males exposed to AAS compared with males treated with vehicle, independently of age of AAS treatment. However, unlike hamsters exposed to AAS in adulthood, hamsters exposed to AAS during adolescence did not display any submissive or riskassessment behaviors up to 4 weeks after discontinuation of AAS treatment. Thus, AAS have longlasting effects on male sexual and agonistic behaviors, with AAS exposure during adolescence resulting in a more pronounced reduction in submissive behavior compared to AAS exposure in adulthood.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Anabolic steroids have long-lasting effects on male social behaviors

Salas-Ramirez

Montalto

Sisk

2010

Behavioural Brain Research

View full text Add to dashboard Cite

show abstract

“…Other plausible explanations include nandrolone-induced enhancement of dopamine biosynthesis (Kurling et al, 2005), over-expression of dopaminergic receptors (Birgner et al, 2008a), and inhibition of dopamine-metabolizing enzymes activity (Birgner et al, 2008b). In addition, nandrolone decanoate may enhance anxiety behavior in rats by stimulation of brain excitability secondary to down-regulation of serotonin receptor mRNA expression (Ambar and Chiavegatto, 2009).…”

Section: Accepted Manuscriptmentioning

confidence: 97%

Lipoic acid and pentoxifylline mitigate nandrolone decanoate-induced neurobehavioral perturbations in rats via re-balance of brain neurotransmitters, up-regulation of Nrf2/HO-1 pathway, and down-regulation of TNFR1 expression

Ahmed

Awdan

2015

Hormones and Behavior

View full text Add to dashboard Cite

“…Incorporating a further separation step decreases the yield of neurotransmitters and their metabolites to 54-69% (the method consisting of extraction with methanol or ethanol and subsequent solid phase extraction with a C18 stationary phase). The use of perchloric acid resulted in the complete denaturation of proteins and the obtained supernatant did not contain dissolved lipids but a low stability of neurotransmitters has been described in this environment [24], resulting in the method being inaccurate. Recovery values ranged in the interval from 63 to 72%.…”

Section: Sample Preparationmentioning

confidence: 97%

“…A range of solvents can be used for the extraction of substances from brain tissue. Ethanol [23], methanol [15] and perchloric acid [24] are the most commonly utilized solvents. The extraction of analytes using methanol or ethanol occurs, however lipids from the brain tissue are diluted as well.…”

Section: Sample Preparationmentioning

confidence: 99%

LC-ESI-MS-MS Method for Monitoring Dopamine, Serotonin and Their Metabolites in Brain Tissue

et al. 2011

View full text Add to dashboard Cite

A rapid and precise LC-ESI-MS-MS method for the parallel identification and quantification of dopamine, serotonin and their metabolites (homovanillic acid, 3-methoxytyramine, 3,4-dihydroxyphenylacetic acid and 5-hydroxyindolacetic acid) from rat brain tissue without any pre-analysis adjustment of the sample such as preconcentration or derivatization has been developed. In particular, the reaction-monitoring mode was selected for its extremely high degree of selectivity and the stableisotope-dilution assay for its high precision of quantification. Alternation the ionization polarity in the course of mass spectrometry detection enabled to determine substances susceptible to various ionization modes in only one analysis run. This fact, in combination with an easy pretreatment step, constitutes the method straightforward and time undemanding. The developed method was characterized with a high precision (B19.5%, determined as RSD), an acceptable accuracy (C82.0%, determined as recovery), a low limit of detection (B0.40 ng/100 mg brain tissue) and a low limit of quantification (B0.42 ng/100 mg brain tissue). The method has been applied in a recent animal study. The levels of the studied neurotransmitters have been determined in the rat brain hippocampus, prefrontal cortex, and striatum in an animal model of schizophrenia induced by an acute dose of a dizocilpine.

show abstract

The anabolic androgenic steroid nandrolone decanoate affects mRNA expression of dopaminergic but not serotonergic receptors

Cited by 27 publications

References 59 publications

Anabolic steroids have long-lasting effects on male social behaviors

Anabolic steroids have long-lasting effects on male social behaviors

Lipoic acid and pentoxifylline mitigate nandrolone decanoate-induced neurobehavioral perturbations in rats via re-balance of brain neurotransmitters, up-regulation of Nrf2/HO-1 pathway, and down-regulation of TNFR1 expression

LC-ESI-MS-MS Method for Monitoring Dopamine, Serotonin and Their Metabolites in Brain Tissue

Contact Info

Product

Resources

About