The amphipod Parhyale hawaiensis as a promising model in ecotoxicology

Santos, Amanda dos; Botelho, Marina Tenório; Vannuci-Silva, Monizze; Artal, Mariana Coletty; Vacchi, Francine Inforçato; Magalhães, Gabriel Rampazzo; Gomes, Vicente; Henry, Theodore B.; Umbuzeiro, Gisela de Aragão

doi:10.1016/j.chemosphere.2022.135959

Cited by 6 publications

(2 citation statements)

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“…This study provides key additional information for future immune system and toxicological evaluations using P. hawaiensis; this organism is already being used as a model in ecotoxicology (dos Santos, Botelho, et al, 2022). Future studies could include the analysis of gene expression alterations in response to stressors to complement the evaluation of hemocytes (Artal et al, 2020), especially now that substantial information on immune gene families in P. hawaiensis has been published (Lai & Aboobaker, 2017).…”

Section: Discussionmentioning

confidence: 99%

“…Because of the increasing interest in the use of the marine amphipod Parhyale hawaiensis in ecotoxicology (Artal et al, 2018, 2019; Botelho et al, 2022; dos Santos, Botelho, et al, 2022; Dos Santos, Vannuci‐Silva, et al, 2022; Vacchi et al, 2019; Vannuci‐Silva et al, 2018, 2019) and in other sciences (Paris et al, 2022; Rehm et al, 2009), we here describe their hemocyte characteristics and identify the various cell types and their proportions, as well as their ability to phagocytize bacteria.…”

Section: Introductionmentioning

confidence: 99%

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Characterization of hemocytes from the marine amphipod Parhyale hawaiensis (Dana 1853): Setting the basis for immunotoxicological studies

Santos

Botelho

Joviano

et al. 2023

Invertebrate Biology

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Hemocytes are circulating blood cells that play a crucial function in amphipods and other crustacean immune systems. The hemocytes of the marine tropical amphipod Parhyale hawaiensis have been used for the evaluation of DNA damage and micronuclei, but they have not been characterized in the scientific literature.The aim of this study was to describe the hemolymph cells of P. hawaiensis and study their phagocytotic activity. Basic dyes were used to differentiate the cell types and the presence of lipids. The total hemocyte counts (THCs) and the proportion and sizes of the hemocyte types were determined. Hemolymph was exposed to Escherichia coli for verification of the presence of phagocytosis. Three cell types, all containing lipids, were identified in P. hawaiensis: granulocytes (oval shape, 13.4 Â 7.6 μm), semi-granulocytes (oval shape, 14.1 Â 7.2 μm), and hyalinocytes (round shape, 9.6 Â 7.2 μm). Those three cell types were found in different percentages in males (64.8%, 31.1%, and 4.2%) and females (70.1%, 28.2%, and 1.7%). THCs for males were 9007 ± 3800 cells per individual and 4695 ± 1892 cells per individual for females. The cells of E. coli were phagocytized by the hemocytes. Our findings increased the knowledge of hemocytes in P. hawaiensis and is a step forward in using hemocyte-based immune responses as an endpoint in ecotoxicology.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Characterization of hemocytes from the marine amphipod Parhyale hawaiensis (Dana 1853): Setting the basis for immunotoxicological studies

Santos

Botelho

Joviano

et al. 2023

Invertebrate Biology

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Sediment Toxicity Tests: A Critical Review of Their use in Environmental Regulations

Leppanen,

Sourisseau,

Burgess

et al. 2024

Enviro Toxic and Chemistry

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Sediments are an integral component of aquatic systems, linking multiple water uses, functions, and services. Contamination of sediments by chemicals is a worldwide problem, with many jurisdictions trying to prevent future pollution (prospective) and manage existing contamination (retrospective). The present review assesses the implementation of sediment toxicity testing in environmental regulations globally. Currently, the incorporation of sediment toxicity testing in regulations is most common in the European Union (EU), North America, and Australasian regions, with some expansion in Asia and non‐EU Europe. Employing sediment toxicity testing in prospective assessments (i.e., before chemicals are allowed on the market) is most advanced and harmonized with pesticides. In the retrospective assessment of environmental risks (i.e., chemicals already contaminating sediments), regulatory sediment toxicity testing practices are applied inconsistently on the global scale. International harmonization of sediment toxicity tests is considered an asset and has been successful through the widespread adoption and deployment of Organisation for Economic Co‐operation and Development guidelines. On the other hand, retrospective sediment assessments benefit from incorporating regional species and protocols. Currently used toxicity testing species are diverse, with temperate species being applied most often, whereas test protocols are insufficiently flexible to appropriately address the range of environmental contaminants, including nanomaterials, highly hydrophobic contaminants, and ionized chemicals. The ever‐increasing and ‐changing pressures placed on aquatic resources are a challenge for protection and management efforts, calling for continuous sediment toxicity test method improvement to insure effective use in regulatory frameworks. Future developments should focus on including more subtle and specific toxicity endpoints (e.g., incorporating bioavailability‐based in vitro tests) and genomic techniques, extending sediment toxicity testing from single to multispecies approaches, and providing a better link with ecological protection goals. Environ Toxicol Chem 2024;00:1–20. © 2024 The Authors. Environmental Toxicology and Chemistry published by Wiley Periodicals LLC on behalf of SETAC.

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Some like it hot: Thermal preference of the groundwater amphipod Niphargus longicaudatus (Costa, 1851) and climate change implications

Di Cicco,

Di Lorenzo,

Fiasca

et al. 2023

Journal of Thermal Biology

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The amphipod Parhyale hawaiensis as a promising model in ecotoxicology

Cited by 6 publications

References 72 publications

Characterization of hemocytes from the marine amphipod Parhyale hawaiensis (Dana 1853): Setting the basis for immunotoxicological studies

Characterization of hemocytes from the marine amphipod Parhyale hawaiensis (Dana 1853): Setting the basis for immunotoxicological studies

Sediment Toxicity Tests: A Critical Review of Their use in Environmental Regulations

Some like it hot: Thermal preference of the groundwater amphipod Niphargus longicaudatus (Costa, 1851) and climate change implications

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