Niche differences in four species of Galium were quantified by using discriminant function analysis ( D F A ) of site characteristics including biotic variables. Data were analyzed separately for a mesic hardwood site and cedar barrens. Significant differences in niche centroids were also determined for 17 co-occurring herbs in the mesic hardwood site using similar variables. Variables used in the analysis included site characteristics such as aspect and percent slope, biotic variables such as total woody basal area and litter composition, and soil characteristics (for the seventeen species) including pH and texture. Biotic variables were included as indicators of environmental variables and possible allelopathic influence.In the Galium data, variables highly correlated with discriminant axes included canopy density, litter type, and overstory type. In the co-occurring species data, litter composition, slope position, soil pH and texture, and steepness of slope were most correlated with discriminant axes. Discriminant axes derived from the Galium cedar barren data set proved a poor predictor of species occurrence in the mesic hardwood site.The utility of D F A in plant niche analysis is discussed.