The ammonical silver reaction for basic nuclear proteins in the spermatids of the mouse

Kusanagi, Akio; Yanagibashi, Kazutoshi

doi:10.1007/bf01418592

Cited by 6 publications

(3 citation statements)

References 16 publications

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“…This may produce artifactual microporosities that give rise to false-positive results. We hypothesize that nanoleakage observed in the hybrid layers created by self-etch adhesives with the use of conventional acidic silver nitrate may be eliminated by the use of a basic version of ammoniacal silver nitrate (Kusanagi and Yanagibashi, 1975). Thus, the null hypothesis of this study was that there is no difference in expression of nanoleakage in self-etch adhesives when resin-dentin interfaces are challenged with a conventional or an ammoniacal version of silver nitrate.…”

Section: Introductionmentioning

confidence: 87%

Two Modes of Nanoleakage Expression in Single-step Adhesives

2002

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Self-etch adhesives that etch, prime, and bond simultaneously should not exhibit incomplete resin infiltration within hybrid layers. We hypothesized that nanoleakage patterns in these systems are artifacts caused by mineral dissolution in mildly acidic silver nitrate. Resin-dentin interfaces bonded with four single-step, self-etch adhesives were examined for nanoleakage by conventional (pH 4.2) and basic ammoniacal (pH 9.5) silver nitrate and prepared for transmission electron microscopy. All adhesives exhibited a reticular mode of nanoleakage within hybrid layers when conventional silver nitrate was used. With ammoniacal silver nitrate, an additional spotted pattern of nanoleakage was observed within adhesive and hybrid layers. The reticular mode of nanoleakage in self-etch adhesives probably represents sites of incomplete water removal that leads to regional suboptimal polymerization. The spotted pattern identified with the use of ammoniacal silver nitrate probably represents potentially permeable regions in the adhesive and hybrid layers that result from the interaction of the basic diamine silver ions with acidic/hydrophilic resin components.

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Section: Introductionmentioning

confidence: 87%

Two Modes of Nanoleakage Expression in Single-step Adhesives

2002

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“…This study correlated the microtensile bond strengths (mTBS) of Prompt L-Pop achieved after one or two-layers of adhesive application, with differences in ultrastructure and nanoleakage within the bonded interfaces as observed by transmission electron microscopy (TEM). To avoid the possibility of inadvertent mineral dissolution with an acidic silver nitrate (AgNO 3 ) tracer solution, we employed a modified silver-staining technique that consisted of the use of a basic version of ammoniacal AgNO 3 (pH 9.5) [11]. The null hypothesis tested was that there is no difference between the location of the air inhibition layer and the mTBS and extent of silver uptake within the resin -dentin interfaces after one or two-layer application of this selfetching, all-in-one adhesive.…”

Section: Introductionmentioning

confidence: 99%

Effects of one versus two applications of an unfilled, all-in-one adhesive on dentine bonding

Pashley

Agee

Pashley

et al. 2002

Journal of Dentistry

171

140

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“…The reaction products of chromatin with silver are electron-opaque, round particles. McRae and Metz (1970) reported particles of 20 to 50 nm in diameter, whereas Kusanagi and Yanagibashi (1975) claimed that the particles were 10 to 30 nm in diamter. We found that the reaction of BrdU-substituted chroma tin with silver led to the formation of two distinct types of particles; one of 2 to 3 nm and another of 6 to 10 nm in diameter.…”

Section: Resultsmentioning

confidence: 99%

Electron microscopy of sister chromatid exchanges

Goyanes

Schvartzman

1983

Cytogenet Genome Res

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BrdU-substituted chromosomes of Don cells were differentially stained with ammoniacal silver carbonate and analyzed by electron microscopy. The results obtained led us to the following conclusions: (1) the differential impregnation of BrdU-substituted chromatids with silver is mainly due to a preferential extraction of non-histone proteins caused by UV-irradiation and incubation in SSC at 55 °C prior to silver staining; (2) the core-like structures detected after silver staining may well represent an artifact due to the effects of severe hypotonic pre-treatment on chromatin dispersion; (3) in each sister-chromatid exchange, the border of exchange clearly defines the course of DNA fibers within the chromatid. This border of exchange tends to run perpendicular to the chromosome axis but may vary slightly depending on the degree of chromosome condensation.

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The ammonical silver reaction for basic nuclear proteins in the spermatids of the mouse

Cited by 6 publications

References 16 publications

Two Modes of Nanoleakage Expression in Single-step Adhesives

Two Modes of Nanoleakage Expression in Single-step Adhesives

Effects of one versus two applications of an unfilled, all-in-one adhesive on dentine bonding

Electron microscopy of sister chromatid exchanges

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