The amino-terminal sequence ofStreptomyces griseus carboxypeptidase

Strydom, Daniël J.; Bazzone, Thomas J.

doi:10.1007/bf01025260

Cited by 3 publications

(4 citation statements)

References 17 publications

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“…Sequencing very small amounts of protein or peptide on the Beckman 890C instrument was another challenge that was addressed by using clean reagents, implementing suggestions for greater efficiency (Frank, 1979), and detecting manually converted PTH-amino acids by high-sensitivity HPLC (Strydom & Bazzone, 1985). Many of the peptides examined were ultimately sequenced by using nanomole amounts of material.…”

Section: Discussionmentioning

confidence: 99%

“…Anilinothiazolinones were converted to phenylthiohydantoins (Strydom & Bazzone, 1985;Fett et al, 1985a), and the PTH-amino acids were identified and quantitated by HPLC using one or both of two separation systems. System 1 used an IBM octadecylsilane column (25 X 0.4 cm, 5-µ particles) at room temperature.…”

Section: Methodsmentioning

confidence: 99%

“…The amino acid sequence of angiogenin isolated from this cell line is described in an accompanying paper (Strydom et al, 1985).…”

Section: Introductionmentioning

confidence: 99%

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Amino acid sequence of human tumor derived angiogenin

Dj¹,

Jw²,

Rr³

et al. 1985

Biochemistry

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361

311

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The amino acid sequence and disulfide bond pairing of human tumor derived angiogenin, the first tumor angiogenesis factor to be isolated in pure form from human sources, have been determined by conventional sequencing techniques adapted and applied to nanomole and subnanomole levels of material. Angiogenin, obtained from conditioned media of a human colonic adenocarcinoma cell line, is a single-chain protein consisting of 123 amino acids with the following sequences: less than Glu1-Asp-Asn-Ser-Arg-Tyr-Thr-His- Phe-Leu-Thr-Gln-His-Tyr-Asp15-Ala-Lys-Pro-Gln-Gly-Arg-Asp-Asp- Arg-Tyr-Cys-Glu-Ser-Ile-Met30- Arg-Arg-Arg-Gly-Leu-Thr-Ser-Pro-Cys-Lys-Asp-Ile-Asn-Thr- Phe45-Ile-His-Gly-Asn-Lys-Arg-Ser -Ile-Lys-Ala-Ile-Cys-Glu-Asn-Lys60-Asn-Gly-Asn-Pro-His-Arg-Glu-Asn -Leu-Arg-Ile -Ser-Lys-Ser-Ser75 -Phe-Gln-Val-Thr-Thr-Cys-Lys-Leu-His-Gly-Gly-Ser-Pro-Trp-Pro90-Pro -Cys-Gln-Tyr -Arg-Ala-Thr-Ala -Gly-Phe-Arg-Asn-Val-Val-Val105-Ala-Cys-Glu-Asn-Gly-Leu-Pro-Val- His-Leu-Asp-Gln-Ser-Ile-Phe120-Arg-Arg-Pro123-OH. Three disulfide bonds link the half-cystinyl residues 26-81, 39-92, and 57-107. The sequence is homologous to that of the pancreatic ribonucleases with 35% identity and many of the remaining residues conservatively replaced. Similarities are especially apparent around the major active-site residues His-12, Lys-41, and His-119 of ribonuclease which are conserved as are three of the four disulfide bonds.(ABSTRACT TRUNCATED AT 250 WORDS)

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Amino acid sequence of human tumor derived angiogenin

Dj¹,

Jw²,

Rr³

et al. 1985

Biochemistry

Self Cite

361

311

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“…All spectra were recorded using the same spectrometer parameters of 50 eV pass energy and a 6-mm slit width. To minimize the influence of the X-rays on the samples, all samples were cooled to liquid nitrogen temperature and spectra were recorded in less than 30 min each [3]. Data were recorded with the sample surface normal to the electron analyzer and for some samples with an angle of 30" to the analyzer to determine the compositions of the first few atomic layers on the AgBr surface.…”

Section: P S Measurementsmentioning

confidence: 99%

An XPS investigation of silver bromide‐coated ion‐selective electrodes

Strydom

Staden

Strydom³

1991

Electroanalysis

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X P S data of AgBr-coated ion-selective electrodes exposed to high concentrations of Ag+ , C1-, Br-, I -, and NH, revealed a change in the surface properties of the original electrode. A 40 min to one week exposure of the silver bromide ion-selective electrode surface to solutions containing high concentrations of chloride ions leads to the formation of a mixed halide layer, as the chloride ions are incorporated in the surface. Exposure to high concentrations of iodide-containing solutions results in a new silver iodide layer on top of the original silver bromide layer. Silver ions difhse to the newly formed layers. NH, results in the rapid degradation of the AgE3r surface as the diamine complex, Ag(NH,):, is formed.1040-0397/91/$3,50 t .25

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Chapter 4 Carboxypeptidase A

Auld

Vallee

1987

Hydrolytic Enzymes

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The amino-terminal sequence ofStreptomyces griseus carboxypeptidase

Cited by 3 publications

References 17 publications

Amino acid sequence of human tumor derived angiogenin

Amino acid sequence of human tumor derived angiogenin

An XPS investigation of silver bromide‐coated ion‐selective electrodes

Chapter 4 Carboxypeptidase A

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