The amino acid sequence and properties of an edema-inducing Lys-49 phospholipase A2 homolog from the venom of Trimeresurus mucrosquamatus

Liu, Chen-Sheng; Chen, Jinmei; Chang, Chen-Hsien; Chen, Shun-Wen; Teng, Che-Ming; Tsai, Inn-Ho

doi:10.1016/0167-4838(91)90552-b

Cited by 59 publications

(26 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Interestingly, two of the untranslated PLA 2 s, CRV-K49 and CRV-R6 (Fig. 1), are structurally almost identical to the abundantly expressed K49-PLA 2 [18] and PLA 2 III in Trimeresurus mucrosquamatus venom, respectively (GenBank accession no. X77088).…”

Section: Untranslated Pla 2 Mrnasmentioning

confidence: 99%

“…The effect of the venom PLA 2 on the ADP-induced platelet aggregation was studied on an aggregometer (Payton, module 600B, Canada) [18].…”

Section: Platelet Inhibition By the Acidic Pla 2 Smentioning

confidence: 99%

“…The hind-paw edema of a rat (female Wistar, 200±250 g) was measured with a plethysmometer (Ugo Basile, model 7150). Extent of hind-paw swelling was calculated based on the paw volume after the injection with native or chemically modified PLA 2 in contrast to buffer injected in the control experiment [18].…”

Section: Binding Of Pla 2 To Heparin and Its Effect On Edema-inductionmentioning

confidence: 99%

See 2 more Smart Citations

Phospholipases A₂ from Callosellasma rhodostoma venom gland

Tsai

Wang

et al. 2000

European Journal of Biochemistry

Self Cite

View full text Add to dashboard Cite

Callosellasma rhodostoma (Malayan pitviper) is a monotypic Asian pitviper of medical importance. Three acidic phospholipases A 2 (PLA 2 s) and one basic PLA 2 -homolog were purified from its venom while 10 cDNAs encoding distinct PLA 2 s were cloned from venom glands of a Thailand specimen of this species. Complete amino-acid sequences of the purified PLA 2 s were successfully deduced from their cDNA sequences. Among the six un-translated PLA 2 cDNAs, two apparently result from recombination of its Lys49-PLA 2 gene with its Asp49-PLA 2 genes. The acidic PLA 2 s inhibit platelet-aggregation, while the noncatalytic PLA 2 -homolog induces local edema. This basic PLA 2 -homolog contains both Asp49 and other, unusual substitutions unique for the venom Lys49-PLA 2 subtype (e.g. Leu5, Trp6, Asn28 and Arg34). Three-dimensional modelling of the basic protein revealed a heparin-binding region, and an abnormal calcium-binding pocket, which may explain its low catalytic activity. Oxidation of up to six of its Met residues or coinjection with heparin reduced its edemainducing activity but methylation of its active site His48 did not. The distinct Arg/Lys-rich and Met-rich region at positions 10±36 of the PLA 2 homolog presumably are involved in its heparin-binding and the cell membraneinterference leading to edema and myotoxicity.

show abstract

Section: Untranslated Pla 2 Mrnasmentioning

confidence: 99%

“…The effect of the venom PLA 2 on the ADP-induced platelet aggregation was studied on an aggregometer (Payton, module 600B, Canada) [18].…”

Section: Platelet Inhibition By the Acidic Pla 2 Smentioning

confidence: 99%

Section: Binding Of Pla 2 To Heparin and Its Effect On Edema-inductionmentioning

confidence: 99%

See 1 more Smart Citation

Phospholipases A₂ from Callosellasma rhodostoma venom gland

Tsai

Wang

et al. 2000

European Journal of Biochemistry

Self Cite

View full text Add to dashboard Cite

show abstract

“…Taiwanese Viperid Snakes and the Correlation to Venom Diversity and Evolution 73 1993) and C-lectin like integrin-agonist on the platelet (Huang et al, 2004); it also causes myonecrosis due to the effects of high content of N6-PLAs (Chen et al 1994;Tsai et al, 1995) and K49-PLAs (Liu et al, 1991). Envenoming by D. acutus causes severe hemorrhage and coagulopathy due to the synergism of both the Zn +2 -metalloproteases and the thrombin like fibrinogenase (Wang et al, 2001), and rich contents of Clectin like antiplatelet proteins (Chen et al, 2000;Tani et al, 2002).…”

Section: Generated By Techbooks Automated Layout Systemmentioning

confidence: 99%

“…We have previously purified, cloned, and characterized PLAs from the venom of Taiwan vipers, including D. r. siamensis (Tsai et al, 1996;Wang et al, 1992), P. mucrosquamatus (Liu et al, 1991;Tsai et al, 1995;Tsai et al, 2001;Wang and Tsai, 1994), D. acutus (Wang et al, 1996a;Wang et al, 1996b), and T. stejnegeri (Tsai et al, 2004). One of the most common components found in the pit viper venoms are the acidic Glu6-PLAs (Wang et al, 1999;Tsai et al, 2004), which may inhibit the aggregation of platelets or play other roles (Tsai et al, 2003).…”

Section: E New Tree Of Acidic Glu6-plasmentioning

confidence: 99%

Revised Systematics of Taiwanese Viperid Snakes and the Correlation to Venom Diversity and Evolution

Tsai

2005

Toxin Reviews

Self Cite

View full text Add to dashboard Cite

Purification and characterization of myotoxin IV, a phospholipase A₂ variant, from Bothrops asper snake venom

et al. 1995

View full text Add to dashboard Cite

A basic myotoxic protein was purified from Bothrops asper venom. Like other basic Bothrops myotoxins, myotoxin IV induces acute muscle damage after intramuscular injection in mice and disrupts negatively charged liposomes but not positively charged ones. Furthermore, this protein exerts a weak anticoagulant effect only at concentrations of 40 micrograms/ml or higher, and is devoid of phospholipase A2 activity. Rabbit polyclonal antibodies to B. asper myotoxin II, a related lysine-49 isoform, cross-react strongly with myotoxin IV by enzyme immunoassay, indicating a high degree of antigenic similarity between these two proteins. The fact that both toxins have similar amino acid compositions and amino-terminal sequences, including leucine-5 and glutamine-11, 2 amino acid residues conserved in all lysine-49 phospholipase A2 variants purified, strongly suggests that B. asper myotoxin IV is a lysine-49 phospholipase A2.

show abstract

The amino acid sequence and properties of an edema-inducing Lys-49 phospholipase A2 homolog from the venom of Trimeresurus mucrosquamatus

Cited by 59 publications

References 23 publications

Phospholipases A₂ from Callosellasma rhodostoma venom gland

Phospholipases A₂ from Callosellasma rhodostoma venom gland

Revised Systematics of Taiwanese Viperid Snakes and the Correlation to Venom Diversity and Evolution

Purification and characterization of myotoxin IV, a phospholipase A₂ variant, from Bothrops asper snake venom

Contact Info

Product

Resources

About

The amino acid sequence and properties of an edema-inducing Lys-49 phospholipase A2 homolog from the venom of Trimeresurus mucrosquamatus

Cited by 59 publications

References 23 publications

Phospholipases A2 from Callosellasma rhodostoma venom gland

Phospholipases A2 from Callosellasma rhodostoma venom gland

Revised Systematics of Taiwanese Viperid Snakes and the Correlation to Venom Diversity and Evolution

Purification and characterization of myotoxin IV, a phospholipase A2 variant, from Bothrops asper snake venom

Contact Info

Product

Resources

About

Phospholipases A₂ from Callosellasma rhodostoma venom gland

Phospholipases A₂ from Callosellasma rhodostoma venom gland

Purification and characterization of myotoxin IV, a phospholipase A₂ variant, from Bothrops asper snake venom