The AMIGO1 adhesion protein activates Kv2.1 voltage sensors

Abstract

Voltage-gated potassium (Kv) channels sense voltage and facilitate transmembrane flow of K+ to control the electrical excitability of cells. The Kv2.1 channel subtype is abundant in most brain neurons and its conductance is critical for homeostatic regulation of neuronal excitability. Many forms of regulation modulate Kv2.1 conductance, yet the biophysical mechanisms through which the conductance is modulated are unknown. Here, we investigate the mechanism by which the neuronal adhesion protein AMIGO1 modulates Kv2.1 channels. With voltage clamp recordings and spectroscopy of heterologously expressed Kv2.1 and AMIGO1 in mammalian cell lines, we show that AMIGO1 modulates Kv2.1 voltage sensor movement to change Kv2.1 conductance. AMIGO1 speeds early voltage sensor movements and shifts the gating charge-voltage relationship to more negative voltages. Fluorescence measurements from voltage sensor toxins bound to Kv2.1 indicate that the voltage sensors enter their earliest resting conformation, yet this conformation is less stable upon voltage stimulation. We conclude that AMIGO1 modulates the Kv2.1 conductance activation pathway by destabilizing the earliest resting state of the voltage sensors.