The amelioration effect of tranexamic acid in wrinkles induced by skin dryness

Ultraviolet B eye irradiation aggravates atopic dermatitis via adrenocorticotropic hormone and NLRP3 inflammasome in NC/Nga mice

“…The supernatant from each sample was stored at −80°C until analysis. Western blotting was carried out as previously described . Briefly, membranes were incubated at 25°C for 1 hour with primary antibodies against the neutrophil marker Gr‐1/Ly‐6G (1:1000; R & D Systems); nucleotide‐binding domain, leucine‐rich‐containing family, pyrin domain‐containing (NLRP)3 (1:1000; Abnova, Taipei, Taiwan); caspase‐1 (1:1000; Epitomics, Burlingame, CA, USA); and β‐actin (1:5000; Sigma‐Aldrich, St. Louis, MO, USA).…”

Ultraviolet A eye irradiation ameliorates colon carcinoma induced by azoxymethane and dextran sodium sulfate through β‐endorphin and methionine‐enkephalin

“…N-(4-amino-2-methylquinolin-6-yl)-2-(4-ethylphenoxymethyl) benzamide monohydrochloride (JTC-801) treatment JTC-801 (1 mg/kg/day; Selleck Chemicals, Houston, TX, USA), an antagonist of opioid receptor, was suspended in a 1 : 175 solution of DMSO : PBS, which was administered orally every other day over 18 weeks following DSS treatment (30).…”

Gp91phox NADPH oxidase modulates litter size by regulating mucin1 in the uterus of mice

“…Female C57BL/6j mice (SLC, Hamamatsu, Japan) and C57BL/6j gp91phox −/mice (Jackson Laboratories, Bar Harbor, ME, USA) were used. The rearing conditions of mice were previously described [Hiramoto et al 2016a]. We allocated six pregnant mice per group and recorded the number of births for each animal.…”

“…The samples were homogenized in lysis buffer (Kurabo, Osaka, Japan) and centrifuged at 12,000 x g for 10 min. The details of the western blotting procedure were described previously [Hiramoto et al 2016a]. Briefly, proteins were transferred to membranes, which were incubated with primary antibodies against NLRP3 (1:1000; Abnova, Taipei, Taiwan), caspase-1 (1:1000; Epitomics, Figure 8.…”