The ameliorating effects of polyamine supplement on physiological and biochemical parameters of Stevia rebaudiana Bertoni under cold stress

Abstract

Stevia rebaudiana Bertoni is a perennial medicinal herb indigenous to tropical regions of South America. The present study evaluated some physiological changes of the plant under cold in the absence and the presence of polyamine conditions. The results showed photosynthetic pigments and photochemical efficiency of PSII reduced in cold treatment conditions than control, significantly. However, a considerable increase in the parameters was observed in all of polyamine treated goups. Leaf compatible solutes such as proline and glycine betaine that were not shown significantly increasing at cold treatment groups, elevated in the 2 days cold-polyamine-treated group. The similar results were seen for free amino acid and total protein contents of the treated plants. It was also revealed that specific activity of some antioxidant enzymes such as catalase and ascorbate peroxidase increased in cold-treated or cold + polyamine-treated groups than related controls. It caused a significant decrease in H 2 O 2 and malondialdehyde contents of the treated plants. The results indicated an increasing in saturated long chain fatty acids after 4 days of cold at 4 °C. It was concluded that the polyamine supplement can induced a considerable tolerance in stevia plants at cold condition.

Foliar Application of Polyamines Modulates Winter Oilseed Rape Responses to Increasing Cold

“…So, these results suggest that PAs take part in winter oilseed rape frost tolerance regulation. This is in agreement with other studies showing alleviation of chilling stress (6 • C) by Put in Anthurium andraeanum [62] and the increase of cold tolerance in stevia plants by PA supplementation [36]. Despite that, PA application determined a higher survival rate of N oilseed rape under increasing frost conditions, yet the rates of survival of PA-treated plants did not reach the level of A plants.…”

“…PAs are known to be involved in plant development, cell division, embryogenesis, fruit development, dormancy termination, regulation of aging, and temperature stress processes [1,33,34]. Many authors indicate that PA accumulation is an important component of a response to cold stress in different crop plant species and that PAs play a significant role in counteracting stress [33,[35][36][37]. Studies of transgenic plants showed that the overproduction of PAs coincided with better stress tolerance [38].…”

Polyamine Function in Plants: Metabolism, Regulation on Development, and Roles in Abiotic Stress Responses

“…Treatment with 1.0 mmol/L Put had the strongest effect, and chilling damage was reduced by treatment with D-Arg (Sun et al, 2018b). Similar results were obtained for stevia plants, where PA supplementation increased their tolerance to cold conditions (Peynevandi et al, 2018). When an SPDS cDNA from Cucurbita ficifolia was introduced into Arabidopsis (Kasukabe et al, 2004), the transgenic plants exhibited a significant increase in SPDS activity and Spd content in leaves together with enhanced tolerance to various stresses including chilling and freezing (Groppa and Benavides, 2008).…”

Targeting Glycinebetaine for Abiotic Stress Tolerance in Crop Plants: Physiological Mechanism, Molecular Interaction and Signaling

“…This evidently illustrates the tripartite regulation of PAs, GB, and brassinosteroid in generating stress-induced responses. Synthetic PA (diamino hydroxyl ethyl phospho mineral and diaminohexanoic mineral amino ethanoic acid) supplementation of the perennial medicinal herb, Stevia rebaudiana, increased the endogenous GB and Pro contents on exposure to cold stress [139].…”