The Ambivalent Role of Glutathione in the Protection of Dna Against Singlet Oxygen

Lafleur, M.V.M.; Hoorweg, J. J.; Joenje, Hans; Westmijze, E.J.; Retèl, J.

doi:10.3109/10715769409056550

Cited by 64 publications

(37 citation statements)

References 17 publications

(2 reference statements)

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“…Also, the inhibitory effect of roscovitine must be considered not only on the cdk but also on RNA synthesis (Ljungman & Paulsen 2001). Because GSH is involved in multiple functions, including DNA and protein synthesis (Lafleur et al 1994), it could be expected that oocytes treated with roscovitine showed a higher GSH content than their counterparts, as was observed in our case since this functional activity of GSH was probably decreased.…”

Section: Discussionsupporting

confidence: 64%

Birth of piglets after transferring of in vitro-produced embryos pre-matured with R-roscovitine

Coy¹,

Romar²,

Ruiz³

et al. 2005

Reproduction

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The objectives of this study were to evaluate: (1) the nuclear maturation, (2) the intracellular glutathione (GSH) content, (3) the normality of fertilization and (4) full development after transplantation of embryos derived from porcine oocytes precultured with 50 mmol/l roscovitine (an inhibitor of p34 cdc2 /cyclin B kinase) for 22 h. After treatment with roscovitine, the nuclear configuration of oocytes (Hoechst staining) was comparable with those examined just after collection: the majority of oocytes were arrested at the germinal vesicle (GV) 1 stage (63.2%). Roscovitine-treated oocytes progressed through meiosis to the metaphase II stage in a conventional step-wise in vitro maturation (IVM) program for 44 h in a proportion similar to control ones (> 85.0%). When roscovitine-treated oocytes and non-treated oocytes were matured for 44 h and then co-cultured with fresh spermatozoa for 18 h, no differences were observed in oocyte penetrability, proportion of monospermic penetration and male pronuclear formation (>87%). Roscovitine increased the GSH synthesis in oocytes at 22 h, whereas, after 44 h, roscovitine-treated oocytes had similar amounts of GSH to non-treated oocytes. Finally, surgical transfer of zygotes at 22-24 h post-insemination, derived from roscovitine-treated oocytes, resulted in one pregnancy with 12 piglets born; control non-treated zygotes resulted in one pregnancy and 10 piglets born. The full-term developmental ability of mammalian oocytes pre-cultured with roscovitine prior to IVM is thereby demonstrated. This validation is important before the introduction of roscovitine into routine procedures.Reproduction (2005) 129 747-755

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Section: Discussionsupporting

confidence: 64%

Birth of piglets after transferring of in vitro-produced embryos pre-matured with R-roscovitine

Coy¹,

Romar²,

Ruiz³

et al. 2005

Reproduction

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“…Several studies have shown that the addition of cysteamine to the oocyte maturation medium increases intracytoplasmic GSH levels in different species [14,17,18,[26][27][28]. GSH, the major non-protein sulphydryl compound in mammalian cells, is an endogenous and ubiquitous reducing agent that protects cells from oxidation and plays a number of important roles in cellular metabolism including protein synthesis and the reduction of disulphides [29]. In oocytes, intracytoplasmic GSH participates in the transformation of the fertilising sperm head in the male pronucleus.…”

Section: Discussionmentioning

confidence: 99%

Developmental competence of prepubertal goat oocytes selected with brilliant cresyl blue and matured with cysteamine supplementation

et al. 2003

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-The aim of this study was to assess the effect of oocyte selection using the brilliant cresyl blue (BCB) test plus the addition of cysteamine to the in vitro maturation (IVM) medium to improve the in vitro embryo development of prepubertal goat oocytes. The oocytes were exposed to 26 µM BCB and classified according to their cytoplasm coloration: BCB+ (oocytes with blue cytoplasm) and BCB-(unstained oocytes). The oocytes were matured in a conventional IVM medium supplemented with cysteamine 100 µM. The control group consisted of oocytes not exposed to BCB and matured without cysteamine. The IVM-oocytes were inseminated and cultured in synthetic oviductal fluid (SOF) for 7 days. The normal fertilisation rate (oocytes showing 2 pronuclei and 1 sperm tail) of BCB+ oocytes (40%) was higher than those of BCB-(21%) and control oocytes (22%). The percentage of morulae plus blastocysts was higher (P < 0.05) in the BCB+ group than in the BCB-group (23.8 vs. 5.1%, respectively). In conclusion, the integration of the BCB test and the addition of cysteamine in the protocol of in vitro embryo production from prepubertal goat oocytes has improved the developmental rates of embryo development. embryo / IVF / IVM / thiol

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“…Multiple actions have been described for GSH, including increasing amino acid transport, stimulating DNA and protein synthesis, reduction of disulfides and protection against toxic effects of oxidative damage (Meister, 1983;Lafleur et al, 1994). It has been demonstrated that adding beta m e r c a p t o e t h a n o l ( M E ) i n c r e a s e d intracellular GSH in the mo use lymphocytes (Zmuda and Friedenson, 1983).…”

Section: Introductionmentioning

confidence: 99%

Effect of Culture Medium Supplementation with b-mercaptoethanol and Amino Acid on Canine Intergeneric Embryo Development with Porcine Oocyte Cytoplasm Recipient

Fibrianto

2015

Indones. J. Biotechnol.

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The present study investigated the effect of culture medium supplementation with mercaptoethanol ( ME)and amino acid (AA) on canine intergeneric embryo development with porcine oocyte cytoplasm. Porcine cumulusoocyte complexes (COCs) were collected from slaughterhouse and matured in TCM-199 supplemented with 26.2mM NaHCO, 3.05 mM glucose, 0.91 mM sodium pyruvate, 0.57 mM L-cysteine, 75 mg/l kanamycin, 10 ng/ml 3epidermal growth factor, equine chorionic gonadotropin (eCG), 10 IU/ml human chorionic gonadotropin (hCG),and 10% (v/v) porcine follicular fluid (pFF) at 39 °C in a humidified atmosphere of 5% CO for 42-44 h and donor cell 2collected from ear skin afghanhound male dog. After somatic cell nuclear transfer (SCNT), embryo developmentwere examined for cleavage rate and 144 hr for final development after cultured in media. The result shows that,amino acid and mercapoethanol addition in culture medium (NCSU-23) have no effect on embryo development.The development rate of embryo until 16 cell stage in NCSU and NCSU supplement are 4.67% and morula stage are3.73% and 4.67%.Key words : intergeneric clone embryo, canine, ( amino acid (AA)

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The Ambivalent Role of Glutathione in the Protection of Dna Against Singlet Oxygen

Cited by 64 publications

References 17 publications

Birth of piglets after transferring of in vitro-produced embryos pre-matured with R-roscovitine

Birth of piglets after transferring of in vitro-produced embryos pre-matured with R-roscovitine

Developmental competence of prepubertal goat oocytes selected with brilliant cresyl blue and matured with cysteamine supplementation

Effect of Culture Medium Supplementation with b-mercaptoethanol and Amino Acid on Canine Intergeneric Embryo Development with Porcine Oocyte Cytoplasm Recipient

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