The Amaranthus leucocarpus Lectin Enhances the Anti-CD3 Antibody-Mediated Activation of Human Peripheral Blood CD4+ T Cells

Urrea, Francisco; Ortiz‐Quintero, Blanca; Sánchez‐García, Francisco Javier; Blanco-Favéla, Francisco; Garfias, Yonathan; Lascuraín, Ricardo; Zenteno, Edgar

doi:10.1620/tjem.221.271

Cited by 4 publications

(5 citation statements)

References 34 publications

(48 reference statements)

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“…Although these responses were slightly lower than the ones observed after CD28 costimulation, the proliferation and activation patterns were the same between cells costimulated with ALL and anti-moesin. These results are in agreement with previous experiments reporting ALL costimulatory capacity in mouse and human T cells (15,16) and that O-moesin is located in lipid rafts, cell membrane structures fundamental for the formation of the immunological synapse (20). Our observations reinforce other works describing moesin as a fundamental molecule for T cell homeostasis, maturation, and function: moesin -/mice exhibit T cells with reduced activation capacity and IL-2 production; lymphopenia as a consequence of a diminished incapacity to egress from the thymus, CD8 + Treg cells from these animals show decreased proliferation (30)(31)(32) and an X-linked moesin-associated immunodeficiency where diminished T cell proliferation is observed has been described in humans (48).…”

Section: Discussionsupporting

confidence: 94%

“…Earlier reports from our group showed that mouse and human CD4 + T cells stimulated in vitro with anti-CD3 and the Amaranthus leucocarpus lectin (ALL) activate and proliferate similarly to anti-CD3/CD28 activated cells (15,16), suggesting that other molecules can generate initial costimulatory signals and, potentially, the existence of an alternate T cell co-activation mechanism. ALL identifies O-glycoproteins with a Galb1-3GalNAc-O-Ser/Thr sequence, even in the presence of sialic acid residues in their structure (17).…”

Section: Introductionmentioning

confidence: 99%

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Expression Dynamics of the O-Glycosylated Proteins Recognized by Amaranthus leucocarpus Lectin in T Lymphocytes and Its Relationship With Moesin as an Alternative Mechanism of Cell Activation

et al. 2021

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T lymphocyte activation begins with antigen/MHC recognition by the TCR/CD3 complex followed by a costimulatory signal provided by CD28. The search for novel costimulatory molecules has been extensive due to their potential use as immunotherapeutic targets. Although some molecules have been identified, they are unable to provide sustainable signaling to allow for proper T cell activation and proliferation. It has been shown that the Amaranthus leucocarpus lectin (ALL) can be used as an in vitro costimulator of CD4+ lymphocytes in the presence of anti-CD3 mAb; this lectin specifically recognizes O-glycans of the Galβ1-3GalNAc-O-Ser/Thr type, including a 70-kDa moesin-like protein that has been suggested as the costimulatory molecule. However, the identity of this molecule has not been confirmed and such costimulation has not been analyzed in CD8+ lymphocytes. We show herein that the expression kinetics of the glycoproteins recognized by ALL (gpALL) is different in CD4+ and CD8+ T cells, unlike moesin expression. Results from IP experiments demonstrate that the previously described 70-kDa moesin-like protein is an O-glycosylated form of moesin (O-moesin) and that in vitro stimulation with anti-CD3 and anti-moesin mAb induces expression of the activation molecules CD69 and CD25, proliferation and IL-2 production as efficiently as cells costimulated with ALL or anti-CD28. Overall, our results demonstrate that O-moesin is expressed in CD4+ and CD8+ T lymphocytes and that moesin provides a new costimulatory activation signal in both T cell subsets.

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Section: Discussionsupporting

confidence: 94%

Section: Introductionmentioning

confidence: 99%

Expression Dynamics of the O-Glycosylated Proteins Recognized by Amaranthus leucocarpus Lectin in T Lymphocytes and Its Relationship With Moesin as an Alternative Mechanism of Cell Activation

et al. 2021

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“…Previous studies on the effects of ConA and Morniga M on lymphocytes [ 50 , 53 ] showed that the recognition of N -glycans by these lectins accounts for the T cell responses. Interestingly, O -glycan-binding lectins may also induce responses in lymphocytes [ 54 , 55 ]. The effect of lectins on adaptive immune cells is usually approached by analyzing the occurrence of lymphoproliferative responses [ 56 , 57 , 58 , 59 , 60 , 61 ], which we previously verified in ArtinM-stimulated murine spleen cells [ 24 ].…”

Section: Discussionmentioning

confidence: 99%

“…These results show that ArtinM acts as an antigen-independent mitogen, a property already attributed to ConA vis-a-vis its ability to induce the proliferation of CD4 + and CD8 + T cells [ 62 ]. Interestingly, ArtinM alone induced the activation of CD4 + and CD8 + T cells, whereas Amaranthus leucocarpus lectin was reported to enhance the anti-CD3 antibody-mediated activation of human peripheral blood CD4 + and CD8 + T cells [ 55 , 63 ]. Beyond cell proliferation, ArtinM also promoted the expression of CD25, which is an activation-associated molecule, on CD4 + and CD8 + T cells.…”

Section: Discussionmentioning

confidence: 99%

ArtinM Mediates Murine T Cell Activation and Induces Cell Death in Jurkat Human Leukemic T Cells

Silva

Oliveira-Brito

Gonçalves

et al. 2017

IJMS

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The recognition of cell surface glycans by lectins may be critical for the innate and adaptive immune responses. ArtinM, a d-mannose-binding lectin from Artocarpus heterophyllus, activates antigen-presenting cells by recognizing TLR2 N-glycans and induces Th1 immunity. We recently demonstrated that ArtinM stimulated CD4+ T cells to produce proinflammatory cytokines. Here, we further studied the effects of ArtinM on adaptive immune cells. We showed that ArtinM activates murine CD4+ and CD8+ T cells, augmenting their positivity for CD25, CD69, and CD95 and showed higher interleukin (IL)-2 and interferon (IFN)-γ production. The CD4+ T cells exhibited increased T-bet expression in response to ArtinM, and IL-2 production by CD4+ and CD8+ T cells depended on the recognition of CD3εγ-chain glycans by ArtinM. The ArtinM effect on aberrantly-glycosylated neoplastic lymphocytes was studied in Jurkat T cells, in which ArtinM induced IL-2, IFN-γ, and IL-1β production, but decreased cell viability and growth. A higher frequency of AnnexinV- and propidium iodide-stained cells demonstrated the induction of Jurkat T cells apoptosis by ArtinM, and this apoptotic response was reduced by caspases and protein tyrosine kinase inhibitors. The ArtinM effects on murine T cells corroborated with the immunomodulatory property of lectin, whereas the promotion of Jurkat T cells apoptosis may reflect a potential applicability of ArtinM in novel strategies for treating lymphocytic leukemia.

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“…In this context, O-glycosylation has been proposed to play a direct and powerful role in regulating T-cell function [14, 25, 26]. Recently, ALL has also shown a costimulatory effect on human CD4 + T cell activated via CD3 [27] turning ALL into a new tool to study O-glycans-bearing glycoproteins in T-cell populations. Thus, the aim of this work was to know whether the O-glycosidically linked structures recognized by ALL are expressed by a Treg subset.…”

Section: Introductionmentioning

confidence: 99%

O-Glycosylation of NnTreg Lymphocytes Recognized by theAmaranthus leucocarpusLectin

Jiménez-Martínez

Lascuraín

Méndez-Reguera³

et al. 2013

Clinical and Developmental Immunology

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O-glycosidically-linked glycans have been involved in development, maturation, homing, and immune regulation in T cells. Previous reports indicate that Amaranthus leucocarpus lectin (ALL), specific for glycans containing galactose-N-acetylgalactosamine and N-acetylgalactosamine, recognizes human naïve CD27+CD25+CD4+ T cells. Our aim was to evaluate the phenotype of CD4+ T cells recognized by ALL in peripheral blood mononuclear cells obtained from healthy volunteers. CD4+ T cells were isolated by negative selection using magnetic beads-labeled monoclonal antibodies; the expression of T regulatory cell phenotypic markers was assessed on ALL-recognized cells. In addition, IL-4, IL-10, IFN-γ, and TGF-β intracellular production in ALL + cells was also evaluated. The analyses of phenotypic markers and intracellular cytokines were performed through flow cytometry. ALL-recognized CD4+ T cells were mainly CD45RA+, CCR7+ cells. Although 52 ± 10% CD25+Foxp3+ cells were positive to ALL, only 34 ± 4% of ALL + cells corresponded to CD25+Foxp3− cells. Intracellular cytokines in freshly obtained ALL +CD4+ T cells exhibited 8% of IL-4, 15% of IL-10, 2% of IFN-γ, and 15% of TGF-β, whereas ALL −CD4+ T cells depicted 1% of IL-4, 2% of IL-10, <1% of IFN-γ, and 6% of TGF-β. Our results show that galactose-N-acetylgalactosamine and N-galactosamine-bearing CD4+ T cells expressed phenotypic markers of NnTreg cells.

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The Amaranthus leucocarpus Lectin Enhances the Anti-CD3 Antibody-Mediated Activation of Human Peripheral Blood CD4+ T Cells

Cited by 4 publications

References 34 publications

Expression Dynamics of the O-Glycosylated Proteins Recognized by Amaranthus leucocarpus Lectin in T Lymphocytes and Its Relationship With Moesin as an Alternative Mechanism of Cell Activation

Expression Dynamics of the O-Glycosylated Proteins Recognized by Amaranthus leucocarpus Lectin in T Lymphocytes and Its Relationship With Moesin as an Alternative Mechanism of Cell Activation

ArtinM Mediates Murine T Cell Activation and Induces Cell Death in Jurkat Human Leukemic T Cells

O-Glycosylation of NnTreg Lymphocytes Recognized by theAmaranthus leucocarpusLectin

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