The Aluminum Cryo-plate Increases Efficiency of Cryopreservation Protocols for Potato Shoot Tips

Yamamoto, Shinichi; Wunna,; Rafique, Tariq; Arizaga, Miriam Valle; Fukui, Kuniaki; Gutiérrez, Esmeralda Judith Cruz; Martinez, Carlos Roman Castillo; Watanabe, Kazuo; Niino, Toshiki

doi:10.1007/s12230-014-9425-5

Cited by 49 publications

(31 citation statements)

References 9 publications

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“…Results of new and promising cryopreservation methods, like the dehydration (D)- and vitrification (V)-plate protocols, show high recovery rates of 70–93.3% (13 accessions; D cryoplate) and 93.3–100% (16 accessions; V cryoplate) (Yamamoto et al 2015; Valle et al 2017), but the assessment of these methods on a larger scale (for example, > 500 randomly selected accessions) has not yet been performed. It is also important to highlight that CIP’s potato cryobank exclusively conserves landraces, while other collections contain higher quantities of improved potato varieties or breeding material, which tend to be less variable and have a narrower genetic base than landraces.…”

Section: Discussionmentioning

confidence: 99%

“…Recently, researchers have published other promising methods for potato cryopreservation, such as the D and V cryoplate. In these methods, shoot tips are “stuck” on special aluminum plates with uniform wells using sodium alginate to facilitate uniform sample handling, reduced risk of damage, and reduced loss of shoot tips (Yamamoto et al 2015; Valle et al 2017). …”

Section: Introductionmentioning

confidence: 99%

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A large-scale viability assessment of the potato cryobank at the International Potato Center (CIP)

Vollmer

Villagaray

Cárdenas

et al. 2017

In Vitro Cell.Dev.Biol.-Plant

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

A large-scale viability assessment of the potato cryobank at the International Potato Center (CIP)

Vollmer

Villagaray

Cárdenas

et al. 2017

In Vitro Cell.Dev.Biol.-Plant

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“…1A1). The base D cryo-plate protocol was derived from the protocol developed by Niino et al (2014) and Yamamoto et al (2015) as follows: 1.…”

Section: Base D Cryo-plate Protocolmentioning

confidence: 99%

“…), elimination of the cold acclimatization step may be possible (Kim et al, 2006;Yamamoto et al, 2015).…”

Section: Optimization Of the Revised D Cryo-plate Protocol Cold Hardementioning

confidence: 99%

Improvement to the D Cryo-plate Protocol Applied to Practical Cryopreservation of <i>In Vitro</i> Grown Potato Shoot Tips

Arizaga¹,

Navarro²,

Martinez³

et al. 2017

The Hortic J

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We modified the D cryo-plate protocol by paper mounting to the cryo-plate with alginate gel and shoot tips, and cryopreserved shoot tips of 13 potato genotypes (potato genetic resources in Mexico) using the revised and optimized D cryo-plate protocol. There were no significant differences in regrowth of cryopreserved shoot tips by addition of the paper mounting step to the base D cryo-plate protocol, besides a reduction in dropping shoot tips in steps during the whole procedure. Some steps of revised D cryo-plate protocol were optimized or reconfirmed, and the effect of optimizations such as cold-hardening, loading solution treatment, and postrewarming treatment on the regeneration of shoot tips was studied. This optimized protocol was successfully applied to 'B-71-240-2' and 12 additional potato genotypes, resulting in 70.0%-93.3% regrowth with an average of 82.8% and stable storage for 1 year. When introducing new cryopreservation techniques, modification and optimization of the method are required to adjust to each laboratory's circumstances. This optimized D cryo-plate method will facilitate cryobanking of potato and other plant genetic resources in Mexico for long-term preservation in a genebank.

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“…A esta temperatura todas las funciones celulares se suspenden y por lo tanto el tejido puede ser almacenado sin cambios o deterioro por largos periodos (Kaczmarczyk et al, 2012). En Solanum tuberosum se han probado exitosamente diversos métodos criogénicos, como encapsulación-deshidratación, vitrificación y D-crioplaca ( Hao et al, 2002;Halmagyi et al, 2005;Hirai, 2011;Yamamoto et al, 2015). Wang et al (2006), propusieron el uso de métodos criogénicos para la conservación a largo plazo de accesiones de papa y para la eliminación de virus.…”

Section: Introductionunclassified

Ácido salicílico induce tolerancia al estrés por criogenia en Solanum tuberosum

Hernández

Sáenz

Gutiérrez

et al. 2019

Remexca

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Un problema común en los procesos criogénicos es la supervivencia a temperatura ultra baja (-196 °C). Con base a los efectos fisiológicos del ácido salicílico (AS) en la señalización de respuestas de tolerancia a estrés, el objetivo fue evaluar el efecto de AS en el incremento de la supervivencia y en el crecimiento de yemas de papa sometidas a criogenia. Microplantas del clon avanzado 06-27 del Banco de Germoplasma in vitro del Programa de Papa en Metepec Estado de México, México, fueron incubadas por 28 días en AS (0, 10-6 y 10-5 M) y sometidas posteriormente a proceso criogénico. Efecto de AS en regeneración de plantas: se observó un incremento significativo en la supervivencia en plantas pretratadas con AS 10-5 y 10-6 M con respecto al testigo (1.66-2.04 veces respectivamente). El peso fresco, longitud de tallo y raíz incrementaron significativamente en microplantas pretratadas con AS con respecto al testigo. Criogenia: ambas concentraciones de AS 10-5 y 10-6 M indujeron significativamente mayor supervivencia (2.17-3.21 veces respectivamente) de explantes a criogenia. Plantas preincubadas en AS y regeneradas después de ser sometidas 1 h a criogenia incrementaron significativamente la longitud de raíz. El testigo presentó formación de callo, ausente en las plantas tratadas con AS. La combinación AS-criogenia favoreció el desarrollo de plantas sometidas a métodos que emplean temperaturas ultra bajas en papa con fines de criopreservación de germoplasma o en crioterapia para la obtención de materiales libres de virus.

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The Aluminum Cryo-plate Increases Efficiency of Cryopreservation Protocols for Potato Shoot Tips

Cited by 49 publications

References 9 publications

A large-scale viability assessment of the potato cryobank at the International Potato Center (CIP)

A large-scale viability assessment of the potato cryobank at the International Potato Center (CIP)

Improvement to the D Cryo-plate Protocol Applied to Practical Cryopreservation of <i>In Vitro</i> Grown Potato Shoot Tips

Ácido salicílico induce tolerancia al estrés por criogenia en Solanum tuberosum

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