The alteration of mRNA expression of SOD and GPX genes, and proteins in tomato ( Lycopersicon esculentum Mill) under stress of NaCl and/or ZnO nanoparticles

Abstract

Five cultivars of tomato having different levels of salt stress tolerance were exposed to different treatments of NaCl (0, 3 and 6 g L) and ZnO-NPs (0, 15 and 30 mg L). Treatments with NaCl at both 3 and 6 g L suppressed the mRNA levels of superoxide dismutase (SOD) and glutathione peroxidase (GPX) genes in all cultivars while plants treated with ZnO-NPs in the presence of NaCl, showed increments in the mRNA expression levels. This indicated that ZnO-NPs had a positive response on plant metabolism under salt stress. Superior expression levels of mRNA were observed in the salt tolerant cultivars, Sandpoint and Edkawy while the lowest level was detected in the salt sensitive cultivar, Anna Aasa. SDS-PAGE showed clear differences in patterns of protein expression among the cultivars. A negative protein marker for salt sensitivity and ZnO-NPs was detected in cv. Anna Aasa at a molecular weight of 19.162 kDa, while the tolerant cultivar Edkawy had two positive markers at molecular weights of 74.991 and 79.735 kDa.

Impact of Foliar Application of Chitosan Dissolved in Different Organic Acids on Isozymes, Protein Patterns and Physio-Biochemical Characteristics of Tomato Grown under Salinity Stress

“…However, the lowest intensity level of SOD isozymes was observed in plants treated with NaCl alone and S + Ch MAL. The results are in line with [ 95 ] who noticed that treating tomato plants with NaCl suppressed mRNA levels of SOD genes, whereas plants treated with zinc oxide nanoparticle inducers, in the presence of NaCl, showed an increase in mRNA expression levels, suggesting the beneficial impact of zinc oxide on plant metabolism under salt stress. Similarly, the present data indicate that chitosan, especially when dissolved in ascorbic acid had a positive response on tomato metabolism under salinity stress.…”

Exogenous Salicylic acid Ameliorates the Adverse Effects of Salt Stress on Antioxidant System in Rosmarinus officinalis L.

“…Therefore, SOD is considered as one of the most effective and functional antioxidant enzymes in limiting oxidative damage (Asada, 1999 andDaCosta &Huang, 2007). The increment in the expression level of SOD gene yet could also be as a result of increased stability of transcribed mRNAs (Soydam et al, 2013 andAlharby et al, 2016). Furthermore, studies on the alleviative influence of various compounds in stressed plants, confirmed the increase in SOD expression as one of the most important mechanisms to improve the effects of saline stress (Zhu &Scandalios, 1994 andSakamoto et al, 1995).…”

Biosynthesis of ZnO Nanoparticles Using Bacillus Subtilis: Characterization and Nutritive Significance for Promoting Plant Growth in Zea mays L

“…Seeds of Zea mays L. were primed through soaking in different concentrations (2, 4, 8,16 mg/L) of biosynthesized ZnO nanoparticles under optimized aeration conditions for 24 h. 25 Seeds priming was followed by washing with distilled water and air drying under shade. These seeds were germinated in Petri plates that were filled with soil and farmyard manure (in a ratio of 1:6) in germination chamber at 25 o C and watered daily.…”