The Allplex 2019-nCoV (Seegene) assay: which performances are for SARS-CoV-2 infection diagnosis?

Farfour, Éric; Lesprit, Philippe; Visseaux, Benoît; Pascreau, Tiffany; Jolly, Émilie; Houhou, Nadira; Mazaux, Laurence; Asso–Bonnet, Marianne; Vasse, Marc

doi:10.1007/s10096-020-03930-8

Cited by 66 publications

(64 citation statements)

References 15 publications

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“…Thus, based on the results of this study, we strongly recommend that rRT-PCR assays should validate more brands for RNA extraction kits to deal with the great demand of them for community screenings and possible future outbreaks. Although the Allplex 2019-nCoV assay failed to detect the N gene within the ssRNA, its performance was accurate for clinical samples, as previously reported ( Farfour et al, 2020 ). However, maximal caution is needed when detection of SARS-CoV-2 genes occurs at high Ct values, for which re-testing should be recommended.…”

Section: Discussionsupporting

confidence: 77%

SARS-CoV-2: Comparative analysis of different RNA extraction methods

Ambrosi

Prezioso

Checconi

et al. 2021

Journal of Virological Methods

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Section: Discussionsupporting

confidence: 77%

SARS-CoV-2: Comparative analysis of different RNA extraction methods

Ambrosi

Prezioso

Checconi

et al. 2021

Journal of Virological Methods

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“…Viral RNA from the upper respiratory tract swab samples was extracted using the MagNA Pure 96 System (Roche Diagnostics, Mannheim, Germany), according to the manufacturer's instructions. The resulting samples were tested for the E, RdRp and N genes of SARS-CoV-2 with real-time RT-PCR using theAllplex 2019-nCoV Assay kit (Seegene) 7 with CFX96 Real-Time PCR Detection System (Bio-Rad, Hercules, California, USA) under the following condition: 20 min at 50°C for reverse transcription and 15 min at 95°C for denaturation as the initial step, followed by 45 cycles of 15 s each at 94°C and 30 s at 58°C. A Ct value of <40 was used as the cut-off for determining positivity, according to the manufacturer's instructions.…”

Section: Rt-pcr For Sars-cov-2 Genesmentioning

confidence: 99%

Upper respiratory viral load in asymptomatic individuals and mildly symptomatic patients with SARS-CoV-2 infection

Hyun

Lim

Kim

et al. 2020

Thorax

131

119

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BackgroundAsymptomatic individuals with SARS-CoV-2 infection have viable viral loads and have been linked to several transmission cases. However, data on the viral loads in such individuals are lacking. We assessed the viral loads in asymptomatic individuals with SARS-CoV-2 infection in comparison with those in symptomatic patients with COVID-19.MethodsStudy participants were recruited from a community facility designated for the isolation of patients with mild COVID-19 in South Korea. The presence of symptoms was evaluated with a questionnaire-based survey. Viral loads in the upper respiratory tract were measured with real-time reverse transcription-PCR (RT-PCR) targeting the E, RdRp and N genes of SARS-CoV-2, with a cycle threshold (Ct) value of 40 for determining positivity.ResultsIn 213 patients with SARS-CoV-2 infection, 41 (19%) had remained asymptomatic from potential exposure to laboratory confirmation and admission; of them, 39 (95%) underwent follow-up RT-PCR testing after a median 13 days. In 172 symptomatic patients, 144 (84%) underwent follow-up RT-PCR testing. Twenty-one (54%) asymptomatic individuals and 92 (64%) symptomatic patients tested positive for SARS-CoV-2 at follow-up. Asymptomatic individuals and symptomatic patients did not show any significant differences in the mean Ct values of the E (31.15 vs 31.43; p>0.99), RdRp (32.26 vs 32.93; p=0.92) and N (33.05 vs 33.28; p>0.99) genes.ConclusionApproximately one-fifth of the individuals without severe symptoms were asymptomatic, and their viral loads were comparable to those in symptomatic patients. A large proportion of mildly symptomatic patients with COVID-19 or asymptomatic individuals with SARS-CoV-2 showed persistent positive upper respiratory RT-PCR results at follow-up.

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“…Apart from the possibility to perform the RT-PCR in house using the selected primer pairs and probes, several ready to use kits were developed for RT-PCR performing. One of the most used is the Allplex 2019-nCoV (Seegene, Seoul, South Korea) which includes three different viral targets and a positive control [124]. Another example is represented by the BGI's real-time fluorescent RT-PCR Kit for detecting SARS-CoV-2 that includes one SARS-CoV-2 specific target and an internal control of the reaction (BGI, Cambridge, MA, USA).…”

Section: Nucleic Acid Detection Technologymentioning

confidence: 99%

The Global Emergency of Novel Coronavirus (SARS-CoV-2): An Update of the Current Status and Forecasting

Hozhabri

Piceci‐Sparascio

Sohrabi

et al. 2020

IJERPH

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Over the past two decades, there have been two major outbreaks where the crossover of animal Betacoronaviruses to humans has resulted in severe acute respiratory syndrome coronavirus (SARS-CoV) and Middle East respiratory syndrome coronavirus (MERS-CoV). In December 2019, a global public health concern started with the emergence of a new strain of coronavirus (SARS-CoV-2 or 2019 novel coronavirus, 2019-nCoV) which has rapidly spread all over the world from its origin in Wuhan, China. SARS-CoV-2 belongs to the Betacoronavirus genus, which includes human SARS-CoV, MERS and two other human coronaviruses (HCoVs), HCoV-OC43 and HCoV-HKU1. The fatality rate of SARS-CoV-2 is lower than the two previous coronavirus epidemics, but it is faster spreading and the large number of infected people with severe viral pneumonia and respiratory illness, showed SARS-CoV-2 to be highly contagious. Based on the current published evidence, herein we summarize the origin, genetics, epidemiology, clinical manifestations, preventions, diagnosis and up to date treatments of SARS-CoV-2 infections in comparison with those caused by SARS-CoV and MERS-CoV. Moreover, the possible impact of weather conditions on the transmission of SARS-CoV-2 is also discussed. Therefore, the aim of the present review is to reconsider the two previous pandemics and provide a reference for future studies as well as therapeutic approaches.

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The Allplex 2019-nCoV (Seegene) assay: which performances are for SARS-CoV-2 infection diagnosis?

Cited by 66 publications

References 15 publications

SARS-CoV-2: Comparative analysis of different RNA extraction methods

SARS-CoV-2: Comparative analysis of different RNA extraction methods

Upper respiratory viral load in asymptomatic individuals and mildly symptomatic patients with SARS-CoV-2 infection

The Global Emergency of Novel Coronavirus (SARS-CoV-2): An Update of the Current Status and Forecasting

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