The allelic distribution of a single nucleotide polymorphism in the PDCD5 gene locus of Japanese non-small cell lung cancer patients

Nanba, Kei; Toyooka, Shinichi; Soh, Junichi; Tsukuda, Kazunori; Yamamoto, Hiromasa; Sakai, Akiko; Ouchida, Mamoru; Kobayashi, Naruyuki; Matsuo, Keitaro; Koide, Naoki; Kusaka, Katsuyasu; Shimizu, Kenji; Date, Hiroshi

doi:10.3892/mmr_00000010

Cited by 6 publications

(6 citation statements)

References 16 publications

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“…An analogous study found similar results for this SNP and its influence on susceptibility to chronic myeloid leukemia (14). Despite these findings, a correlation between this particular SNP and lung cancer susceptibility was not seen in a Japanese population (15).…”

Section: Introductionsupporting

confidence: 54%

Construction and characterization of a PDCD5 recombinant lentivirus vector and its expression in tumor cells

et al. 2012

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Abstract. The function and mechanism of the programmed cell death 5 (PDCD5) gene is not completely understood, so it is necessary to build a stable and efficient PDCD5 recombinant lentiviral expression vector. The coding region of the PDCD5 gene was PCR amplified from pCMV-SPORT6. Next, the PDCD5 fragment and the pGC-FU vector were digested with the restriction enzyme AgeI and ligated by in-Fusion technology to build the pGC-FU-PDCD5 plasmid. Competent E. coli DH5α cells were transformed and positive clones were identified by PCR. The PCR products were digested and sequenced. After sequencing, positive clones were selected to grow and propagate. The pGC-FU-PDCD5 plasmid DNA was purified and mixed with the pHelper1.0 and pHelper2.0 packaging plasmids. They were co-transfected into 293T cells. The viral titer was measured by real-time PCR. The expression of GFP and PDCD5 was detected by both Western blotting and fluorescence microscopy. Additionally, the A498, ACHN, HCT116 and LoVo tumor cell lines were transfected with the virus supernatant, and PDCD5 expression was detected in these cells. The constructed pGC-FU-PDCD5 plasmid contained the correct PDCD5 gene sequence. Strong green fluorescence in both the cytoplasm and cell membrane was observed following transfection with purified pGC-FU-PDCD5 into 293T cells. The transfection rate was greater than 95%, and the expression of the PDCD5-GFP fusion protein was confirmed by Western blotting. The titer of the recombinant PDCD5 lentiviral condensed supernatant was measured to be 2x10 9 Tu/ml. The transfection efficiencies of A498, ACHN and HCT116 cells were greater than 90%. However, transfection efficiency of LoVo cells was lower but still greater than 70%. The PDCD5-GFP fusion protein was stable in these transfected tumor cells, as detected by Western blotting. In conclusion, a PDCD5 recombinant lentiviral vector was successfully constructed, and high expression of the plasmid was observed in tumor cells.

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Section: Introductionsupporting

confidence: 54%

Construction and characterization of a PDCD5 recombinant lentivirus vector and its expression in tumor cells

et al. 2012

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“…In a previous study, we also did not find effect on Aβ species and tau/p-tau [9] . These markers, however, may provide novel insight into AD pathology and its progression [16] , [18] , [26] . The biomarkers reflecting specific AD pathology in CSF (Aβ and tau/p-tau) change over the disease course in a pattern that does not reflect neurodegeneration in a simple way making them less suitable for longitudinal markers of disease progression [27] , [28] .…”

Section: Discussionmentioning

confidence: 99%

Effect of physical exercise on markers of neuronal dysfunction in cerebrospinal fluid in patients with Alzheimer's disease

Jensen

Portelius

Høgh

et al. 2017

A&D Transl Res & Clin Interv

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IntroductionPhysical exercise has gained increasing focus as a potential mean to maintain cognitive function in patients with Alzheimer's disease (AD). Alongside the markers of specific AD pathology (amyloid β and tau), other pathologies such as neuronal damage and synaptic loss have been proposed as markers of the disease. Here, we study the effect of physical exercise on biomarkers of neuronal and synaptic integrity.MethodsCerebrospinal fluid (CSF) from 51 AD subjects who participated in the randomized controlled trial Preserving Cognition, Quality of Life, Physical Health and Functional Ability in Alzheimer's Disease: The Effect of Physical Exercise (ADEX) was analyzed for the concentration of neurofilament light (NFL), neurogranin (Ng), visinin-like protein-1 (VILIP-1), and chitinase-3–like protein 1 (YKL-40). Participants were subjected to either 16 weeks of moderate- to high-intensity exercise (n = 25) or treatment as usual (control group, n = 26), and CSF was collected before and after intervention.ResultsNo significant differences in CSF concentrations of VILIP-1, YKL-40, NFL, and Ng were observed when comparing mean change from baseline between the exercise and control groups. Similarly, when classifying subjects based on their exercise levels, no significant changes were observed for the biomarkers in the control group compared with the high-exercise group (attending 80% of the exercise sessions with an intensity of 70% of maximum heart rate or above).DiscussionThese results are not supportive of a modulatory effect of physical exercise on the selected biomarkers of neuronal and synaptic integrity in patients with AD.

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“…Inhibition of these two pathways caused drug induced apoptosis, hence the high presence of f-circRNAs in leukemic cells will have a negative effect on treatment response. This could be an indicator of possible treatment failure for leukemic patients (Nanba and Toyooka, 2008).…”

Section: Several Reported Circrnas Contribute To Treatment Resistance Via Pi3k/akt Pathwaymentioning

confidence: 99%

Circular RNAs: Potential Regulators of Treatment Resistance in Human Cancers

Jeyaraman¹,

Eam²,

Mutalib³

et al. 2020

Front. Genet.

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Circular RNAs (circRNAs) which were once considered as "junk" are now in the spotlight as a potential player in regulating human diseases, especially cancer. With the development of high throughput technologies in recent years, the full potential of circRNAs is being uncovered. CircRNAs possess some unique characteristics and advantageous properties that could benefit medical research and clinical applications. CircRNAs are stable with covalently closed loops that are resistant to ribonucleases, have disease stage-specific expressions and are selectively abundant in different types of tissues. Interestingly, the presence of circRNAs in different types of treatment resistance in human cancers was recently observed with the involvement of a few key pathways. The activation of certain pathways by circRNAs may give new insights to treatment resistance management. The potential usage of circRNAs from this aspect is very much in its infancy stage and has not been fully validated. This mini-review attempts to highlight the possible role of circRNAs as regulators of treatment resistance in human cancers based on its intersection molecules and cancer-related regulatory networks.

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The allelic distribution of a single nucleotide polymorphism in the PDCD5 gene locus of Japanese non-small cell lung cancer patients

Cited by 6 publications

References 16 publications

Construction and characterization of a PDCD5 recombinant lentivirus vector and its expression in tumor cells

Construction and characterization of a PDCD5 recombinant lentivirus vector and its expression in tumor cells

Effect of physical exercise on markers of neuronal dysfunction in cerebrospinal fluid in patients with Alzheimer's disease

Circular RNAs: Potential Regulators of Treatment Resistance in Human Cancers

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