The albino-deletion complex of the mouse: molecular mapping of deletion breakpoints that define regions necessary for development of the embryonic and extraembryonic ectoderm.

Abstract

Previous complementation analyses with five (c11DSD, c5FR60Hg, c2YPSj, c4FR60Hd, c6H) of the mouse albino deletions defined at least two genes on chromosome 7, known as eed and exed, which are necessary for development of the embryonic and extraembryonic ectoderm, respectively, of early postimplantation embryos. The region of chromosome 7 containing these two genes has now been accessed at the molecular level by cloning two of the deletion breakpoint-fusion fragments. The c2YPSj breakpoints were isolated by cloning an EcoRI fragment containing a copy of an albino region-specific repeat unique to c2YPSj DNA. Similarly, the c11DSD breakpoints were isolated by cloning a c11DSD EcoRI fragment detected by a unique-sequence probe mapping proximal to the albino-coat-color locus. By mapping the cloned breakpoints relative to the remaining three deletions, the c11DSD distal breakpoint was found to define the distal limit of the region containing eed, whereas the c2YPSj and c6H distal breakpoints were found to define the proximal and distal limits, respectively, of the region containing exed.

Molecular analysis of 36 mutations at the mouse pink-eyed dilution (p) locus.

“…The mutagenesis strategy being employed is similar to the strategy used to generate a number of such mutations within the 6to 1 1-cM region of chromosome 7included in the more distally mapping d' 6""' deletion (RINCHIK and RUSSELL 1990;RINCHIK and CARPENTER 1993;RINCHIK et al 1993a;POTTER et al 1995). The existence of a highly developed original complementation map of the region surrounding the albino (c) locus (RUSSELL et al 1982), along with constantly evolving molecular deletion maps of subregions of the complex (e.g., RINCHIK and RUS SELL 1990; SHARAN et al. 1991;KELSEY et al 1992;KLEBIC et al 1992;RINCHIK et al 1993b;FAUST et al 1995), has greatly facilitated the genetic and molecular analyses of these new c-region mutations (RINCHIK and CARPENTER 1993;RINCHIK et al 1993a;POTTER et al 1995).…”

Molecular analysis of radiation-induced albino (c)-locus mutations that cause death at preimplantation stages of development.

“…In combination, these facts suggest that these three deletions would be more suitable than the other (longer) deletions affecting pid for defining the extent of pid on any physical map. In this context, it is important to note that the more distal of the two Bex-group deletion breakpoints (c" and c4FR60Hd* , NISWANDER et al 1989NISWANDER et al , 1991SHARAN et al 1991) defines the proximal boundary of the pid locus. Consequently, it will be important to locate the c~~ or c4FR60Hd breakpoint on any physical map extended proximally from D7Rn6, as this would provide the necessary stopping point in the search for the pid gene(s).…”

“…carrying deletion breakpoints of c1lDSD and c2"'j have been cloned (SHARAN et al 1991). Cloning of these breakpoints thus provided initial molecular-access points to the genetic regions required for the development of embryonic and extraembryonic ectoderm (loci eed and exed in Figure 1).…”

Molecular genetics of the brown (b)-locus region of mouse chromosome 4. I. Origin and molecular mapping of radiation- and chemical-induced lethal brown deletions.

“…However, DNAs prepared from animals heterozygous for a prenatally lethal deletion and a M . spretus chromosome have been extremely useful for the molecular mapping of loci within a deletion or a series of deletions (e.g., CHABOT et al 1988;JOHNSON et al 1989;SHARAN et al 1991;NICHOLLS et al 1993). The numerous restriction fragment length variants (RFLVs) that exist between M .…”