The Aggregation-Prone Mutant Huntingtin Protein in a Cellular Context - Approaches by Super-Resolution Imaging

Sahl, Steffen J.; Vonk, Willianne I. M.; Weiss, Lucien E.; Lau, Lana; Frydman, Judith; Moerner, W. E.

doi:10.1016/j.bpj.2013.11.3780

Cited by 1 publication

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“…These pioneering studies have paved the way for more detailed biophysical and biological studies. Notably, the growth (polymerisation) of amyloid species can now be monitored in situ using multi-color SMLM approaches and new insights into the nature of amyloid elongation rates were obtained for α-synuclein [134,135] and polyQ [136][137][138]. Whilst the former studies were performed in vitro, in test tube samples, similar investigations can now be performed directly in cells.…”

Section: Neurodegenerationmentioning

confidence: 99%

From single-molecule spectroscopy to super-resolution imaging of the neuron: a review

Laine

Schierle

Linde³

et al. 2016

Methods Appl. Fluoresc.

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For more than 20 years, single-molecule spectroscopy has been providing invaluable insights into nature at the molecular level. The field has received a powerful boost with the development of the technique into super-resolution imaging methods, ca. 10 years ago, which overcome the limitations imposed by optical diffraction. Today, single molecule super-resolution imaging is routinely used in the study of macromolecular function and structure in the cell. Concomitantly, computational methods have been developed that provide information on numbers and positions of molecules at the nanometer-scale. In this overview, we outline the technical developments that have led to the emergence of localization microscopy techniques from single-molecule spectroscopy. We then provide a comprehensive review on the application of the technique in the field of neuroscience research.

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