The Ageing of Connective Tissue

Verzár, F.

doi:10.1159/000210714

Cited by 81 publications

(25 citation statements)

References 2 publications

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“…During aging and degeneration, the ability of cells to synthesize the correct matrix components appears to be compromised (Zhao et al, 2007). The proportion of ECM components, especially crosslinked collagen fibers (Verzar, 1964), fibronectin (Colige et al, 1992), and laminin (Fodil-Bourahla et al, 1999), increases with age (Labat-Robert, 2004;Labat-Robert and Robert, 2007). However, quantitative and qualitative alterations of ECM components and their influence on cellular fate during aging have not been examined in detail.…”

Section: Discussionmentioning

confidence: 99%

Differential Expression of Extracellular Matrix Proteins in Senescent and Young Human Fibroblasts: a Comparative Proteomics and Microarray Study

Yang

Kwon

Rhim

et al. 2011

Molecules and Cells

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The extracellular matrix (ECM) provides an essential structural framework for cell attachment, proliferation, and differentiation, and undergoes progressive changes during senescence. To investigate changes in protein expression in the extracellular matrix between young and senescent fibroblasts, we compared proteomic data (LTQ-FT) with cDNA microarray results. The peptide counts from the proteomics analysis were used to evaluate the level of ECM protein expression by young cells and senescent cells, and ECM protein expression data were compared with the microarray data. After completing the comparative analysis, we grouped the genes into four categories. Class I included genes with increased expression levels in both analyses, while class IV contained genes with reduced expression in both analyses. Class II and Class III contained genes with an inconsistent expression pattern. Finally, we validated the comparative analysis results by examining the expression level of the specific gene from each category using Western blot analysis and semiquantitative RT-PCR. Our results demonstrate that comparative analysis can be used to identify differentially expressed genes.

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Section: Discussionmentioning

confidence: 99%

Differential Expression of Extracellular Matrix Proteins in Senescent and Young Human Fibroblasts: a Comparative Proteomics and Microarray Study

Yang

Kwon

Rhim

et al. 2011

Molecules and Cells

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“…Several tissue changes have been reported to accompany increased cross-linkage, increased thermal shrinkage (10), decreased swelling in acids ( 11 ), increased tensile strength (12), resistance to proteolysis (13), and altered solubility in weak acid (14). Such changes have been observed in collagen from aged individuals as well as youthful collagen in which cross-linkage has been induced or stabilized with sodium borohydride (12,15,16) or CHOH (8,10).…”

Section: Discussionmentioning

confidence: 99%

Simulation of lung tissue properties in age and irreversible obstructive syndromes using an aldehyde.

Sugihara¹,

Martin²

1975

J. Clin. Invest.

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A B S T R A C T Weak solutions of CHOH alter tissue properties, probably by forming intermolecular crosslinkages. The maximum length (L.a.) to which alveolar wall can be extended is reduced. If exposed to CHOH while extended, the resting length (Lo) of alveolar wall increases. Maximum extensibility (Xma =Lmax/Lo) decreases. Similar changes are found in the alveolar wall of man with aging and are significantly more marked in patients with irreversible obstructive pulmonary syndromes. A reduction in the energy loss of the lengthtension cycle (hysteresis) was seen after exposure to CHOH, however, that does not occur with age or in obstructive syndromes. Because an exposure of alveolar wall to elastase increases Lo and hysteresis, we used a staged exposure to CHOH followed by elastase. Tissue suitably prepared by exposure to CHOH followed by elastolysis better simulates the tissue changes of age and irreversible obstructive syndromes.

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“…The readings obtained were auto matically recorded by a computer and tested for sta tistical significance by the Wilcoxon modification of Student's t lest. 1 The annulus fibrosus of three discs from each ani mal was evaluated by microspcctrophotomctry by taking 8 readings in the dorsal and the same number in the ventral part and proceeding centripetally from the periphery towards the border with the nucleus pulposus. By this method it was possible to separate readings for the outer from those for the inner lamellae.…”

Section: Methodsmentioning

confidence: 99%

Fibronectin Content of the Annulus fibrosus in Diabetic and Non-Diabetic Sand Rats

Silberberg

Meier-Ruge²,

Odermatt³

1989

Pathobiology

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Annulus fibrosus of intervertebral discs from diabetic and non-diabetic sand rats were examined by microspectrophotometry for fibronectin content. This was higher in the diabetic animals both in the dorsal and ventral parts and in the outer and inner lamellae of the annulus. It is suggested that diabetes-related changes in fibronectin are similar to changes in annular collagen observed in species other than sand rats.

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The Ageing of Connective Tissue

Cited by 81 publications

References 2 publications

Differential Expression of Extracellular Matrix Proteins in Senescent and Young Human Fibroblasts: a Comparative Proteomics and Microarray Study

Differential Expression of Extracellular Matrix Proteins in Senescent and Young Human Fibroblasts: a Comparative Proteomics and Microarray Study

Simulation of lung tissue properties in age and irreversible obstructive syndromes using an aldehyde.

Fibronectin Content of the Annulus fibrosus in Diabetic and Non-Diabetic Sand Rats

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