The age-related loss of skeletal muscle mass and function: Measurement and physiology of muscle fibre atrophy and muscle fibre loss in humans

Wilkinson, Daniel J.; Piasecki, Mathew; Atherton, Philip J.

doi:10.1016/j.arr.2018.07.005

Cited by 481 publications

(365 citation statements)

References 148 publications

Supporting

Mentioning

283

Contrasting

Unclassified

Order By: Relevance

“…Nucleation was also affected by the cytokine, since the number of nuclei per visible fiber was reduced from 9 to 5 for the concentration of 80 ng/mL ( Figure 3C). All these morphological changes were consistent with those related to sarcopenia induced by TNF-α 25,26,30,39,40 . In order to test whether functional changes were also compatible, we set the concentration of TNF-α to 40 ng/mL for the remaining experiments, since it was the concentration for which some effects could be observed without becoming too adverse.…”

Section: Resultssupporting

confidence: 85%

3D-printed drug testing platform based on a 3D model of aged human skeletal muscle

Mestre

Garcia

Patiño

et al. 2020

Preprint

View full text Add to dashboard Cite

Three-dimensional engineering of skeletal muscle is becoming increasingly relevant for tissue engineering, disease modeling and bio-hybrid robotics, where flexible, versatile and multidisciplinary approaches for the evaluation of tissue differentiation, functionality and force measurement are required. This works presents a 3D-printed platform of bioengineered human skeletal muscle which can efficiently model the three-dimensional structure of native tissue, while providing information about force generation and contraction profiles. Proper differentiation and maturation of myocytes is demonstrated by the expression of key myo-proteins using immunocytochemistry and analyzed by confocal microscopy, and the functionality assessed via electrical stimulation and analysis of contraction kinetics. To validate the flexibility of this platform for complex tissue modelling, the bioengineered muscle is treated with tumor necrosis factor α to mimic the conditions of aged or senescence-like tissue, which is supported by morphological and functional changes. Moreover, as a proof of concept, the effects of Argireline ® Amplified peptide, a cosmetic ingredient that causes muscle relaxation, are evaluated in both healthy and aged tissue models. Therefore, the results demonstrate that this 3D-bioengineered human muscle platform could be used to assess morphological and functional changes in the aging process of muscular tissue with potential applications in biomedicine, cosmetics and bio-hybrid robotics.

show abstract

Section: Resultssupporting

confidence: 85%

3D-printed drug testing platform based on a 3D model of aged human skeletal muscle

Mestre

Garcia

Patiño

et al. 2020

Preprint

View full text Add to dashboard Cite

show abstract

“…It follows, therefore, that underlying causes of sarcopenia might also contribute to frailty. Sarcopenia is attributable to a decrease in the cross‐sectional area (atrophy) of individual muscle fibres and a decrease in the numbers of muscle fibres (McPhee et al., ; Wilkinson, Piasecki, & Atherton, ). These muscle changes are associated with declining numbers of motor neurons and functioning motor units (MUs; Piasecki et al., ,c; Piasecki, Ireland, Jones, & McPhee, ).…”

Section: Introductionmentioning

confidence: 99%

Frailty phenotype and frailty index are associated with distinct neuromuscular electrophysiological characteristics in men

Święcicka

Piasecki

Stashuk

et al. 2019

Experimental Physiology

View full text Add to dashboard Cite

New Findings What is the central question of this study?Human frailty is characterized by accumulated health complaints, including medical conditions, low physical and psychological function and social components. It is currently unknown whether the condition is associated with neuromuscular changes detectable by electrophysiology obtained from voluntary and involuntary muscle contractions. What is the main finding and its importance?A higher likelihood of frailty was significantly associated with a smaller size of vastus lateralis motor unit potentials during voluntary contractions and smaller compound muscle action potentials generated by electrical stimulation. Importantly, these associations were independent of age and body mass index. Abstract The purpose of this study was to determine whether neuromuscular electrophysiological characteristics that are known to underlie sarcopenia are also associated with the more complex frailty syndrome. Eighty‐six men [mean (SD) age, 74 (8) years] were classed as non‐frail (robust), prefrail or frail using criteria from the frailty phenotype (FP) and the frailty index (FI). The femoral nerve was stimulated maximally and the resulting compound muscle action potential amplitude (CMAP) measured over the vastus lateralis. Motor unit potential (MUP) size was assessed during voluntary contractions using intramuscular electromyography (iEMG). Logistic and negative binomial regression models determined relationships between FP and FI with CMAP and MUP sizes before and after adjustments for age and body mass index. Larger CMAP size was associated with a lower likelihood of frailty in fully adjusted models: a 1SD higher level in vastus lateralis CMAP size was associated with a 0.4 (95% confidence interval: 0.2, 0.6; P < 0.01) unit lower FI (40% of the FI range) and more than halving of the odds [odds ratio: 0.43 (95% confidence interval: 0.21, 0.90)] of having a frail/prefrail phenotype. Greater MUP size was also related to lower FI values using unadjusted and fully adjusted models. However, MUP size was not significantly related to FP in any model. Smaller MUPs and a smaller CMAP were significantly associated with a higher likelihood of frailty, independent of age and body mass index. These results relate neuromuscular electrophysiological characteristics to the complex frailty syndrome and identify motor unit remodelling as a possible contributing factor.

show abstract

“…() and previous reports (Wilkinson et al . ) suggest that myofibre innervation can be preserved at least partly in well‐functioning master athletes. On the other hand, it should be acknowledged that the negative effect of ageing on neuromuscular function remains dominant, as signs of myofibre denervation and decline in motor unit numbers are present even in high‐level master athletes (Hepple & Rice, ; Sonjak et al .…”

Section: Summary Representation Of the Key Findings Reported By Sonjamentioning

confidence: 99%

“…During the last few decades major strides have been made in our knowledge of the regulation of human skeletal muscle mass with ageing (Wilkinson et al . ), while age‐induced changes in human myocellular neuromotor plasticity remain less well documented. Overall, however, it is well known that human ageing results in neuromotor degradation manifesting as a loss of spinal motoneurons and a reduced number of functioning motor units (Manini et al .…”

Section: Summary Representation Of the Key Findings Reported By Sonjamentioning

confidence: 99%

Ageing of human skeletal muscle – myofibre reinnervation as a protective determinant of age‐related loss of physical function

Nielsen

2019

The Journal of Physiology

View full text Add to dashboard Cite

The age-related loss of skeletal muscle mass and function: Measurement and physiology of muscle fibre atrophy and muscle fibre loss in humans

Cited by 481 publications

References 148 publications

3D-printed drug testing platform based on a 3D model of aged human skeletal muscle

3D-printed drug testing platform based on a 3D model of aged human skeletal muscle

Frailty phenotype and frailty index are associated with distinct neuromuscular electrophysiological characteristics in men

Ageing of human skeletal muscle – myofibre reinnervation as a protective determinant of age‐related loss of physical function

Contact Info

Product

Resources

About