The age of the bull influences the transcriptome and epigenome of blastocysts produced by IVF

Wu, Chongyang; Blondin, Patrick; Vigneault, Christian; Labrecque, Rémi; Sirard, Marc‐André

doi:10.1016/j.theriogenology.2019.12.020

Cited by 26 publications

(32 citation statements)

References 50 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Moreover, some of these DMR were mapped to genes that are transcriptionally active during preimplantation stages, suggesting their potential role in early embryo development ( Fang et al, 2019 ). Similarly, age-related DMRs were observed in bovine spermatozoa ( Takeda et al, 2017 , 2019 ; Lambert et al, 2018 ), and 57 of 2223 DMRs (2.56%) were retained in blastocysts ( Lambert et al, 2018 ; Wu et al, 2020 ). Among the genes that are mapped with these DMRs, some of them were involved in spermatogenesis (FKBP6) and embryonic preimplantation development (AKT2).…”

Section: The Known Non-genetic Parental Influences In Dairy Cattlementioning

confidence: 81%

Parental Effects on Epigenetic Programming in Gametes and Embryos of Dairy Cows

Sirard

2020

Front. Genet.

Self Cite

View full text Add to dashboard Cite

The bovine represents an important agriculture species and dairy breeds have experienced intense genetic selection over the last decades. The selection of breeders focused initially on milk production, but now includes feed efficiency, health, and fertility, although these traits show lower heritability. The non-genetic paternal and maternal effects on the next generation represent a new research topic that is part of epigenetics. The evidence for embryo programming from both parents is increasing. Both oocytes and spermatozoa carry methylation marks, histones modifications, small RNAs, and chromatin state variations. These epigenetic modifications may remain active in the early zygote and influence the embryonic period and beyond. In this paper, we review parental non-genetic effects retained in gametes on early embryo development of dairy cows, with emphasis on parental age (around puberty), the metabolism of the mother at the time of conception and in vitro culture (IVC) conditions. In our recent findings, transcriptomic signatures and DNA methylation patterns of blastocysts and gametes originating from various parental and IVC conditions revealed surprisingly similar results. Embryos from all these experiments displayed a metabolic signature that could be described as an “economy” mode where protein synthesis is reduced, mitochondria are considered less functional. In the absence of any significant phenotype, these results indicated a possible similar adaptation of the embryo to younger parental age, post-partum metabolic status and IVC conditions mediated by epigenetic factors.

show abstract

Section: The Known Non-genetic Parental Influences In Dairy Cattlementioning

confidence: 81%

Parental Effects on Epigenetic Programming in Gametes and Embryos of Dairy Cows

Sirard

2020

Front. Genet.

Self Cite

View full text Add to dashboard Cite

show abstract

“…In a previous study, we generated blastocysts in vitro with semen from different bulls of the same age as the ones used in the present study [29]. The transcriptome of these blastocysts was in uenced by paternal age, and canonical pathways related to the DEGs resulted in altered energy production and protein synthesis.…”

Section: Pre-implantation Embryo Developmental Competencementioning

confidence: 93%

“…This assumption was further veri ed by germline-speci c Dicer and Drosha conditional knockout in mice, which resulted in failure of zygotic genome activation and embryonic development [17]. One member of this miRNA family, miR-34b, was differentially expressed in the sperm of young bulls; however, the blastocyst rates were not signi cantly affected [29], indicating that this miRNA does not have a lethal in uence on bovine embryos. Molecular and cellular function analysis using IPA predicts that the differentially expressed sperm-borne miRNAs from young bulls will potentially in uence the gene expression, cellular assembly and organization, and cellular function and maintenance at 2-cell stage, while later at blastocysts stage, these miRNAs will be likely to affect RNA damage and repair, protein synthesis, and small molecule biochemistry (Fig.…”

Section: Function Of Sperm Mirna In Early Embryo Developmentmentioning

confidence: 98%

“…To explore these possibilities, we used the transcriptomic data of blastocysts produced in vitro from sperm of younger bulls [29]. In that study, we used IPA to predict the upstream regulators of the differentially expressed genes (DEGs) in blastocysts from younger bulls in a very similar age design as the present study.…”

Section: Paternal Age Effects On Blastocystsmentioning

confidence: 99%

“…Targets that are presented in both the of up-and down-regulated miRNAs were ltered out. The list of remaining targets was then overlapped with the transcriptomic databases from 2-cell embryos [23] and blastocysts [29]. For the functional prediction of sperm miRNAs in 2-cell embryos, databases of targets expressed at the 2-cell stage and related to 2-cell competence were created for IPA (Supplemental data, tables S3-S6).…”

Section: Bioinformatics Analysis Of Small Rnasmentioning

confidence: 99%

See 2 more Smart Citations

Sperm miRNAs— potential mediators of bull age and early embryo development

Blondin

Vigneault

et al. 2020

Preprint

Self Cite

View full text Add to dashboard Cite

Background: Sperm miRNAs were reported to regulate spermatogenesis and early embryonic development in some mammals including bovine. The dairy cattle breeding industry now tends to collect semen from younger bulls under high selection pressure at a time when semen quality may be suboptimal compared to adult bulls. Whether the patterns of spermatic miRNAs are affected by paternal age and/or impact early embryogenesis is not clear. Hence, we generated small non-coding RNA libraries of sperm collected from same bulls at 10, 12, and 16 months of age, using 16 months as control for differential expression and functional analysis. Results: We firstly excluded all miRNAs present in measurable quantity in oocytes according to the literature. Of the remaining miRNAs, ten sperm-borne miRNAs were significantly differentially expressed in younger bulls (four in the 10 vs 16 months contrast and six in the 12 vs 16 months contrast). Targets of miRNAs were identified and compared to the transcriptomic database of two-cell embryos, to genes related to two-cell competence, and to the transcriptomic database of blastocysts. Ingenuity pathway analysis of the targets of these miRNAs suggested potential influence on the developmental competence of two-cell embryos and on metabolism and protein synthesis in blastocysts. Conclusions: The results showed that miRNA patterns in sperm are affected by the age of the bull and may mediate the effects of paternal age on early embryonic development.

show abstract

Review: The role of male reproductive tract secretions in ruminant fertility

Fernández-Fuertes

2023

animal

View full text Add to dashboard Cite

The age of the bull influences the transcriptome and epigenome of blastocysts produced by IVF

Cited by 26 publications

References 50 publications

Parental Effects on Epigenetic Programming in Gametes and Embryos of Dairy Cows

Parental Effects on Epigenetic Programming in Gametes and Embryos of Dairy Cows

Sperm miRNAs— potential mediators of bull age and early embryo development

Review: The role of male reproductive tract secretions in ruminant fertility

Contact Info

Product

Resources

About