The after-effects of hypoxia exposure on the clam Ruditapes philippinarum in Omaehama beach, Japan

Kozuki, Yasunori; Yamanaka, Ryoichi; Matsushige, Maya; Saitoh, Azusa; Otani, Sosuke; Ishida, Tatsunori

doi:10.1016/j.ecss.2012.08.026

Cited by 21 publications

(8 citation statements)

References 14 publications

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“…To prevent the biological and ecological consequences of hypoxification, it is essential to monitor the coastal environment for the subtle physiological, behavioral, and reproductive aberrations in marine organisms which are relatively more susceptible to the impact of declining dissolvable oxygen. R. philippinarum is a commonly used bioindicator (Ji et al, 2006;Liu et al, 2013;Kozuki et al, 2013) and its sensitivity to hypoxia has previously been demonstrated in the lab and in the field (Kozuki et al, 2013). Our data showed significant alterations in the metabolite profiles of the gills of R. philippinarum under hypoxia, adding a new dimension of impacts that the coastal water hypoxification has on this important aquaculture species.…”

Section: Discussionsupporting

confidence: 60%

“…The irregularity of epithelium and loss of interlamellar tissue of the gill filaments may have significant effects on respiration and , isoleucine; 2, alanine; 3, succinate; 4, betaine; 5, arginine; 6, glycine; 7, tyrosine; 8, phenylalanine; and 9, aspartate. food uptake, as previously reported (Marigómez et al, 2002;Au, 2004;Abdel-Nabi et al, 2007), although food clearance rates were not measured in the current study. In support of this, a previous study showed that repeated exposure to hypoxia reduces the clearance rate (Kozuki et al, 2013).…”

Section: Discussionmentioning

confidence: 54%

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Effects of hypoxia in the gills of the Manila clam Ruditapes philippinarum using NMR-based metabolomics

Zhang

Wei

et al. 2017

Marine Pollution Bulletin

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Coastal hypoxia affects the survival, behavior, and reproduction of individual local marine organisms, and the abundance, biomass, and biodiversity of coastal ecosystems. In this study, we investigated the chronic effects of hypoxia on the metabolomics in the gills of Ruditapes (R.) philippinarum. The results indicated significant alterations in the metabolite profiles in the gills of the hypoxia-treated clams, in comparison with those maintained under normoxia. The levels of betaine, taurine, glycine, isoleucine, and alanine were significantly reduced, suggesting a disturbance of osmotic balance associated with hypoxia. Meanwhile, metabolites involved in energy metabolism, such as alanine and succinate, were also affected. Dramatic histopathological changes were observed in the gills and hepatopancreases of R. philippinarum grown in hypoxic waters, demonstrating tissue damages apparently caused by long-term exposure to hypoxia. Our findings suggest that hypoxia significantly affects the physiology of R. philippinarum, even at a sub-lethal level, and impedes health of the clams.

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Section: Discussionsupporting

confidence: 60%

Section: Discussionmentioning

confidence: 54%

Effects of hypoxia in the gills of the Manila clam Ruditapes philippinarum using NMR-based metabolomics

Zhang

Wei

et al. 2017

Marine Pollution Bulletin

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“…V. philippinarum lives in intertidal or subtidal areas that experience considerable fluctuations in abiotic factors, including temperature and oxygen. We exposed juvenile Manila clams to combinations of two temperatures (20 and 23.5°C) and two DO levels (3.5 and 6–7 mg/L), which model conditions frequently experienced (Kozuki et al., ; Shin, Kim, Chung, & Hur, ; Uzaki, Kai, Aoyama, & Suzuki, ), to determine the effects of combined temperature and DO changes on mortality, emerging behavior from sediment, and oxygen consumption rates. Because clams generally burrow into sediment to escape predation, emerging behavior is a behavioral response to stressors, such as hypoxia and hypercapnia (Lee & Kim, ; Lee, Lee, & Chin, ; Saloom & Duncan, ).…”

Section: Introductionmentioning

confidence: 99%

At the tipping point: Differential influences of warming and deoxygenation on the survival, emergence, and respiration of cosmopolitan clams

Kim

Park

Sin

2018

Ecology and Evolution

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Although warming and low dissolved oxygen (DO) levels are co‐occurring significant climatic stressors in the ocean, the combined effects of these stressors on marine benthic animals have not been well established. Here, we tested the effects of elevated temperatures and low dissolved oxygen levels on the survival, emerging behavior from sediment, and the respiration of juvenile cosmopolitan Manila clams (Venerupis philippinarum) by exposing them to two temperatures (20 and 23.5°C) and DO levels (3.5 and 6–7 mg/L). Although within previously described tolerable ranges of temperature and DO, this 3.5°C increase in temperature combined with a 50% decrease in DO had a devastating effect on the survival of clams (85% mortality after 8 days). The mortality of clams under normoxia at 23.5°C appeared to be higher than under the low DO condition at 20°C. On the other hand, more clams emerged from sediment under the low DO condition at 20°C than under any other conditions. Oxygen consumption rates were not significantly affected by different conditions. Our results suggest temperature elevation combined with low oxygen additively increases stress on Manila clams and that warming is at least as stressful as low DO in terms of mortality. However, low DO poses another threat as it may induce emergence from sediment, and, thus increase predation risk. This is the first evidence that a combination of warming and deoxygenation stressors should reduce population survival of clams much more so than changes in a single stressor.

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“…Dissolved oxygen (DO) in Laizhou Bay and Rushan Bay dropped below 2.0 mg L -1 , the situation was worse in the Xiaoqing River estuary, with DO concentrations of less than 0.5 mg L -1 [14–16]. In Japan, mass mortalities of Manila clam caused by hypoxia have occurred many times during summer, bringing tremendous economic loss to the fishery [17,18].…”

Section: Introductionmentioning

confidence: 99%

“…In addition, different species of bivalves have different levels of tolerance; the LC 50 for Atlantic surf clam Spisula solidissima was measured as 0.5 mg L -1 , while for Baltic clam Macoma balthica it was 1.7 mg L -1 [19,20]. Previous studies have indicated that Manila clams are able to survive more than a week in severe oxygen-deficient conditions [17,18]. However, most experiments with this species have been conducted at a single extremely low DO level, often < 0.5 mg L -1 , and there are no quantitative results for LC 50 or LT 50 .…”

Section: Introductionmentioning

confidence: 99%

Effects of hypoxia on survival, behavior, metabolism and cellular damage of Manila clam (Ruditapes philippinarum)

Sun

Zhang

et al. 2019

PLoS ONE

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The Manila clam Ruditapes philippinarum has become a common and dominant macrobenthic species in coastal areas of the northwestern Pacific and temperate waters of Europe; it is also a major cultured shellfish, with annual worldwide production exceeding 3.3 million tonnes. This species faces greater risk of exposure to hypoxia as eutrophication worsens throughout its coastal habitats; however, its tolerance to hypoxia remains unclear, and the toxicological indicators including LC 50 and LT 50 have not yet been assessed. Previous studies on the effects of hypoxia on marine benthos have focused largely on functional responses, such as metabolism and gene expression, leaving potential structural damage to the mitochondria or the cells unknown. In this study we assessed the effects of hypoxia on Manila clam in terms of survival, behavior, metabolism and cellular damage, using a newly designed automated hypoxia simulation device that features exceptional accuracy and good stability. The clams exhibited strong tolerance to hypoxia as the 20-day LC 50 for dissolved oxygen (DO) was estimated to be 0.57 mg L -1 , and the LT 50 at 0.5 mg L -1 DO was 422 hours. Adaptations included fewer buried clams and a depressed metabolism, while the unexpected rise in the activities of key enzymes involved in glycolysis may indicate a diverse strategy of shellfish under hypoxia. Cellular damage was observed as collapse of the mitochondrial cristae and both cellular and mitochondrial vacuolization. This multi-level study complements and updates our knowledge of the effects of hypoxia on marine benthos, by improving our understanding of the potential for marine ecological transformation under hypoxic conditions and providing useful information for Manila clam farming.

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The after-effects of hypoxia exposure on the clam Ruditapes philippinarum in Omaehama beach, Japan

Cited by 21 publications

References 14 publications

Effects of hypoxia in the gills of the Manila clam Ruditapes philippinarum using NMR-based metabolomics

Effects of hypoxia in the gills of the Manila clam Ruditapes philippinarum using NMR-based metabolomics

At the tipping point: Differential influences of warming and deoxygenation on the survival, emergence, and respiration of cosmopolitan clams

Effects of hypoxia on survival, behavior, metabolism and cellular damage of Manila clam (Ruditapes philippinarum)

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