The aflatoxin-detoxifizyme specific expression in the parotid gland of transgenic pigs

Lou, Angang; Cai, Jiabing; Zhang, Xuemei; Cui, Cheng-dou; Piao, Ying-shu; Guan, Lan

doi:10.1007/s11248-017-0036-z

Cited by 5 publications

(3 citation statements)

References 28 publications

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“…Additionally, as noted by Segard et al (42), autocrine/paracrine GH has been linked to muscle cell proliferation and myotube differentiation. This concept elucidates the observed increase in pectoral muscle fiber density in this experiment, which can be attributed to the elevated expression and Research indicated that increased TP and albumin levels, along with enhanced activities of AST, ALT and AKP, were markers of high protein metabolism in the body (43,44). Conversely, BUN levels had been found to inversely correlate with protein synthesis (45).…”

Section: Discussionsupporting

confidence: 67%

Effects of glycyrrhiza polysaccharides on growth performance, meat quality, serum parameters and growth/meat quality-related gene expression in broilers

Li,

Qin,

et al. 2024

Front. Vet. Sci.

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With growing restrictions on the use of antibiotics in animal feed, plant extracts are increasingly favored as natural feed additive sources. Glycyrrhiza polysaccharide (GP), known for its multifaceted biological benefits including growth promotion, immune enhancement, and antioxidative properties, has been the focus of recent studies. Yet, the effects and mechanisms of GP on broiler growth and meat quality remain to be fully elucidated. This study aimed to investigate the effects of GP on growth, serum biochemistry, meat quality, and gene expression in broilers. The broilers were divided into five groups, each consisting of five replicates with six birds. These groups were supplemented with 0, 500, 1,000, 1,500, and 2,000 mg/kg of GP in their basal diets, respectively, for a period of 42 days. The results indicated that from day 22 to day 42, and throughout the entire experimental period from day 1 to day 42, the groups receiving 1,000 and 1,500 mg/kg of GP showed a significant reduction in the feed-to-gain ratio (F:G) compared to the control group. On day 42, an increase in serum growth hormone (GH) levels was shown in groups supplemented with 1,000 mg/kg GP or higher, along with a significant linear increase in insulin-like growth factor-1 (IGF-1) concentration. Additionally, significant upregulation of GH and IGF-1 mRNA expression levels was noted in the 1,000 and 1,500 mg/kg GP groups. Furthermore, GP significantly elevated serum concentrations of alkaline phosphatase (AKP) and globulin (GLB) while reducing blood urea nitrogen (BUN) levels. In terms of meat quality, the 1,500 and 2,000 mg/kg GP groups significantly increased fiber density in pectoral muscles and reduced thiobarbituric acid (TBA) content. GP also significantly decreased cooking loss rate in both pectoral and leg muscles and the drip loss rate in leg muscles. It increased levels of linoleic acid and oleic acid, while decreasing concentrations of stearic acid, myristic acid, and docosahexaenoic acid. Finally, the study demonstrated that the 1,500 mg/kg GP group significantly enhanced the expression of myogenin (MyoG) and myogenic differentiation (MyoD) mRNA in leg muscles. Overall, the study determined that the optimal dosage of GP in broiler feed is 1,500 mg/kg.

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Section: Discussionsupporting

confidence: 67%

Effects of glycyrrhiza polysaccharides on growth performance, meat quality, serum parameters and growth/meat quality-related gene expression in broilers

Li,

Qin,

et al. 2024

Front. Vet. Sci.

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“…Various physical, chemical, and biological approaches to preventing AFB 1 contamination have been developed [ 13 , 14 , 15 ]. For instance, transgenic pigs expressing aflatoxin-detoxifizyme (ADTZ) exhibit resistance to AFB 1 toxicity [ 16 ]. Nevertheless, economic losses and threats to public health caused by AFB 1 contamination persist [ 13 , 17 ].…”

Section: Introductionmentioning

confidence: 99%

Genome-Scale CRISPR Knockout Screening Identifies BACH1 as a Key Regulator of Aflatoxin B1-Induced Oxidative Damage

Zhang

Zhao

et al. 2022

Antioxidants

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Aflatoxin B1 (AFB1) is amongst the mycotoxins commonly affecting human and animal health, raising global food safety and control concerns. The mechanisms underlying AFB1 toxicity are poorly understood. Moreover, antidotes against AFB1 are lacking. Genome-wide CRISPR/Cas9 knockout screening in porcine kidney cells identified the transcription factor BTB and CNC homolog 1 (BACH1) as a gene required for AFB1 toxicity. The inhibition of BACH1 expression in porcine kidney cells and human hepatoma cells resulted in increased resistance to AFB1. BACH1 depletion attenuates AFB1-induced oxidative damage via the upregulation of antioxidant genes. Subsequently, virtual structural screening identified the small molecule 1-Piperazineethanol, α-[(1,3-benzodioxol-5-yloxy)methyl] -4-(2-methoxyphenyl) (M2) as an inhibitor of BACH1. M2 and its analogues inhibited AFB1-induced porcine and human cell death in vitro, while M2 administration significantly improved AFB1-induced symptoms of weight loss and liver injury in vivo. These findings demonstrate that BACH1 plays a central role in AFB1-induced oxidative damage by regulating antioxidant gene expression. We also present a potent candidate small-molecule inhibitor in developing novel treatments for AFB1 toxicity.

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“…However, feedstuffs may also contain toxic factors such as mycotoxins. To combat these fungal toxins, a bacterial gene that encodes and enzyme that detoxifies aflatoxin was expressed in the salivary gland of first the mouse (Guan et al 2015 ) and then the pig (Lou et al 2017 ). Unlike the experiments above that measured digestive parameters, these experiments required an examination of the impact of aflatoxin on the liver.…”

Section: Digestion Improvement With Genetically Engineered Pigsmentioning

confidence: 99%

Improvements in pig agriculture through gene editing

et al. 2022

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Genetic modification of animals via selective breeding is the basis for modern agriculture. The current breeding paradigm however has limitations, chief among them is the requirement for the beneficial trait to exist within the population. Desirable alleles in geographically isolated breeds, or breeds selected for a different conformation and commercial application, and more importantly animals from different genera or species cannot be introgressed into the population via selective breeding. Additionally, linkage disequilibrium results in low heritability and necessitates breeding over successive generations to fix a beneficial trait within a population. Given the need to sustainably improve animal production to feed an anticipated 9 billion global population by 2030 against a backdrop of infectious diseases and a looming threat from climate change, there is a pressing need for responsive, precise, and agile breeding strategies. The availability of genome editing tools that allow for the introduction of precise genetic modification at a single nucleotide resolution, while also facilitating large transgene integration in the target population, offers a solution. Concordant with the developments in genomic sequencing approaches, progress among germline editing efforts is expected to reach feverish pace. The current manuscript reviews past and current developments in germline engineering in pigs, and the many advantages they confer for advancing animal agriculture.

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The aflatoxin-detoxifizyme specific expression in the parotid gland of transgenic pigs

Cited by 5 publications

References 28 publications

Effects of glycyrrhiza polysaccharides on growth performance, meat quality, serum parameters and growth/meat quality-related gene expression in broilers

Effects of glycyrrhiza polysaccharides on growth performance, meat quality, serum parameters and growth/meat quality-related gene expression in broilers

Genome-Scale CRISPR Knockout Screening Identifies BACH1 as a Key Regulator of Aflatoxin B1-Induced Oxidative Damage

Improvements in pig agriculture through gene editing

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