The advancement of blood cell research by optical tweezers

Avsievich, Tatiana; Zhu, Ruixue; Попов, А. П.; Bykov, Alexander; Meglinski, Igor

doi:10.1016/j.revip.2020.100043

Cited by 50 publications

(37 citation statements)

References 166 publications

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“…It has been proven to be a safe and non-invasive manipulation for living objects such as biomolecules, bacteria, viruses and cells [ 85 , 86 , 87 ]. The advantages of OT over AFM and micropipette aspiration are higher sensitivity, no contamination, whole-cell control in a non-destructive manner and controllable laser light intensity to adjust the applied force with a pN accuracy [ 88 ]. Individually, OT is a great instrument, but when combined with other modalities such as fluorescent microscopy [ 89 ], microfluidics [ 90 ] and Raman spectroscopy [ 91 ], etc., it has enhanced its potentiality for further cellular analysis.…”

Section: Single-cell Mechanical Properties For Mechanotransductionmentioning

confidence: 99%

“…Many such applications of OT have been carried out, such as blood cell studies, adhesion, cell interactions, etc. [ 88 ]. In another study, Gou et al has reported a case study where optical traps were used at various positions of the bone marrow stromal cell layer to gather leukemia cells, thus enabling their interactions and maintaining the cell contact for a few minutes by applying a small trapping force [ 94 ].…”

Section: Single-cell Mechanical Properties For Mechanotransductionmentioning

confidence: 99%

“…(LED—Light emitting diode) ( c ) Model of the trapping process inside the sample chamber. (Reproduced with permission from [ 88 ] under Creative Commons Attribution 4.0 International License).…”

Section: Figurementioning

confidence: 99%

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A Review of Single-Cell Adhesion Force Kinetics and Applications

Shinde

Illath

Gupta

et al. 2021

Cells

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Cells exert, sense, and respond to the different physical forces through diverse mechanisms and translating them into biochemical signals. The adhesion of cells is crucial in various developmental functions, such as to maintain tissue morphogenesis and homeostasis and activate critical signaling pathways regulating survival, migration, gene expression, and differentiation. More importantly, any mutations of adhesion receptors can lead to developmental disorders and diseases. Thus, it is essential to understand the regulation of cell adhesion during development and its contribution to various conditions with the help of quantitative methods. The techniques involved in offering different functionalities such as surface imaging to detect forces present at the cell-matrix and deliver quantitative parameters will help characterize the changes for various diseases. Here, we have briefly reviewed single-cell mechanical properties for mechanotransduction studies using standard and recently developed techniques. This is used to functionalize from the measurement of cellular deformability to the quantification of the interaction forces generated by a cell and exerted on its surroundings at single-cell with attachment and detachment events. The adhesive force measurement for single-cell microorganisms and single-molecules is emphasized as well. This focused review should be useful in laying out experiments which would bring the method to a broader range of research in the future.

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Section: Single-cell Mechanical Properties For Mechanotransductionmentioning

confidence: 99%

Section: Single-cell Mechanical Properties For Mechanotransductionmentioning

confidence: 99%

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A Review of Single-Cell Adhesion Force Kinetics and Applications

Shinde

Illath

Gupta

et al. 2021

Cells

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“…Recently, the active nature of RBC flickering was investigated using active and passive microrheology of a single RBC in a sophisticated four-bead OT configuration [ 21 ]. The advances in OT technology, such as multiple and dynamic optical traps and circular to linear light polarization in OTs, allowed for multiple applications in studies of RBCs, as also shown in two recent reviews [ 22 , 23 ].…”

Section: Introductionmentioning

confidence: 99%

Biomechanics of Ex Vivo-Generated Red Blood Cells Investigated by Optical Tweezers and Digital Holographic Microscopy

Bernecker

Lima

Ciubotaru

et al. 2021

Cells

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Ex vivo-generated red blood cells are a promising resource for future safe blood products, manufactured independently of voluntary blood donations. The physiological process of terminal maturation from spheroid reticulocytes to biconcave erythrocytes has not been accomplished yet. A better biomechanical characterization of cultured red blood cells (cRBCs) will be of utmost interest for manufacturer approval and therapeutic application. Here, we introduce a novel optical tweezer (OT) approach to measure the deformation and elasticity of single cells trapped away from the coverslip. To investigate membrane properties dependent on membrane lipid content, two culture conditions of cRBCs were investigated, cRBCPlasma with plasma and cRBCHPL supplemented with human platelet lysate. Biomechanical characterization of cells under optical forces proves the similar features of native RBCs and cRBCHPL, and different characteristics for cRBCPlasma. To confirm these results, we also applied a second technique, digital holographic microscopy (DHM), for cells laid on the surface. OT and DHM provided related results in terms of cell deformation and membrane fluctuations, allowing a reliable discrimination between cultured and native red blood cells. The two techniques are compared and discussed in terms of application and complementarity.

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“…Furthermore, the formation of blebs (balloon-shaped membrane protrusions with no cytoskeleton content) turns out to be a possible phenomenon with physiological and mechanical implications (SPANGLER et al, 2011;PONTES et al, 2011). Our simulation results reinforce the idea that stress causes membrane rearrangements to adapt to the load and, in particular, that cytoskeleton is dynamic and can rapidly adapt to major changes in the cell shape (SHEETZ; SABLE; DÖBEREINER, 2006;AVSIEVICH et al, 2020). An other speculative explanation is that the effective energy unit k B T should be reduced, resulting in a lower cytoskeleton stiffness (see (RODRÍGUEZ-GARCÍA et al, 2015)).…”

Section: Validation Through Experimental Measurementssupporting

confidence: 75%

A two-component model of the red blood cell membrane and other mathematical models in medicine

Meacci¹

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The advancement of blood cell research by optical tweezers

Cited by 50 publications

References 166 publications

A Review of Single-Cell Adhesion Force Kinetics and Applications

A Review of Single-Cell Adhesion Force Kinetics and Applications

Biomechanics of Ex Vivo-Generated Red Blood Cells Investigated by Optical Tweezers and Digital Holographic Microscopy

A two-component model of the red blood cell membrane and other mathematical models in medicine

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