The advanced strategy for enhancing biobutanol production and high-efficient product recovery with reduced wastewater generation

Xue, Chuang; Zhang, Xiaolin; Wang, Jufang; Xiao, Min; Chen, Lijie; Bai, Feng‐Wu

doi:10.1186/s13068-017-0836-7

Cited by 38 publications

(16 citation statements)

References 45 publications

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“…The sequential overliming and 5.0% AC resulted in remarkable fermentability and high-butanol yield (0.22 g g −1 sugar). This agrees well with a recent report on butanol yield by Clostridium beijerinckii CC101 [ 44 ]. The final concentration of acetone, butanol and ethanol was 7.1, 13.2 and 1.0 g L −1 , respectively.…”

Section: Resultssupporting

confidence: 94%

Effect of overliming and activated carbon detoxification on inhibitors removal and butanol fermentation of poplar prehydrolysates

Zhang

Xia

et al. 2018

Biotechnol Biofuels

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BackgroundBiomass prehydrolysates from dilute acid pretreatment contain a considerable amount of fermentable sugars for biofuels production. However, carbonyl degradation compounds present severe toxicity to fermentation microbes. Furans (such as furfural and hydroxymethylfurfural), aliphatic acids (such as acetic acid, formic acid and levulinic acid) and phenolic compounds (such as vanillin and syringaldehyde) have been suggested to be the main inhibitors in biomass prehydrolysates. However, no single compound has been determined as the dominant toxic inhibitor. The effects of various detoxification methods on inhibitors removal have not been fully understood.ResultsThe effects of overliming and activated carbon (AC) detoxification on the removal of inhibitors and butanol fermentation of the poplar prehydrolysates were investigated. Gas chromatography–mass spectrometry (GC/MS) was used to identify and quantify 46 carbonyl compounds as potential inhibitors. It was observed that overliming and AC treatment alone did not make the prehydrolysates fermentable with Clostridium saccharobutylicum. The sequential overliming and AC resulted in a remarkable fermentability and a high butanol yield at 0.22 g g−1 sugar. The inhibitor removal in the prehydrolysates treated by overliming and AC was also examined by GC/MS. Overliming removed 75.6% of furan derivatives and 68.1% of aromatic monomers. In comparison, AC (5.0% w/v) removed 77.9% of furan derivatives and 98.6% of aromatic monomers. In addition, overliming removed much more 2,5-furandicarboxyaldehyde, 5-ethylfuran-2-carbaldehyde and 2,5-hexanedione than AC did. On the contrary, AC could remove considerably more phenolic acids than overliming. In the sequential detoxification, both dialdehydes/diketones and phenolic acids were extensively removed. This could be the main reason why the sequential detoxification enabled a remarkable ABE fermentation for the prehydrolysates.ConclusionsThis study indicated that the effect of overliming and AC treatment on inhibitors removal was related to their chemical structures. Overliming removed more dialdehydes and diketones than AC treatment, while AC removed more phenolic acids than overliming. Sequential overliming and AC treatment were required to make the prehydrolysates fermentable with C. saccharobutylicum. The study also suggested different detoxification method was needed for ABE fermentation of the prehydrolysate as compared to ethanol fermentation.Electronic supplementary materialThe online version of this article (10.1186/s13068-018-1182-0) contains supplementary material, which is available to authorized users.

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Section: Resultssupporting

confidence: 94%

Effect of overliming and activated carbon detoxification on inhibitors removal and butanol fermentation of poplar prehydrolysates

Zhang

Xia

et al. 2018

Biotechnol Biofuels

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“…In addition, there is still room for a further optimization of the global process to obtain butanol from coffee silverskin. For instance, the adjustment of enzymatic hydrolysis and the concentration of its buffering solution is known to have an important effect on butanol generation [ 46 , 47 ].…”

Section: Resultsmentioning

confidence: 99%

Biobutanol production from coffee silverskin

et al. 2018

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BackgroundCoffee silverskin, a by-product from coffee roasting industries, was evaluated as a feedstock for biobutanol production by acetone–butanol–ethanol fermentation. This lignocellulosic biomass contained approximately 30% total carbohydrates and 30% lignin. Coffee silverskin was subjected to autohydrolysis at 170 °C during 20 min, with a biomass-to-solvent ratio of 20%, and a subsequent enzymatic hydrolysis with commercial enzymes in order to release simple sugars. The fermentability of the hydrolysate was assessed with four solventogenic strains from the genus Clostridium. In addition, fermentation conditions were optimised via response surface methodology to improve butanol concentration in the final broth.ResultsThe coffee silverskin hydrolysate contained 34.39 ± 2.61 g/L total sugars, which represents a sugar recovery of 34 ± 3%. It was verified that this hydrolysate was fermentable without the need of any detoxification method and that C. beijerinckii CECT 508 was the most efficient strain for butanol production, attaining final values of 4.14 ± 0.21 g/L acetone, 7.02 ± 0.27 g/L butanol and 0.25 ± 0.01 g/L ethanol, consuming 76.5 ± 0.8% sugars and reaching a butanol yield of 0.269 ± 0.008 gB/gS under optimal conditions.ConclusionsCoffee silverskin could be an adequate feedstock for butanol production in biorefineries. When working with complex matrices like lignocellulosic biomass, it is essential to select an adequate bacterial strain and to optimize its fermentation conditions (such as pH, temperature or CaCO3 concentration).Electronic supplementary materialThe online version of this article (10.1186/s12934-018-1002-z) contains supplementary material, which is available to authorized users.

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“…[ 12 ] C. beijerinckii is a model solventogenic clostridium that exhibits good tolerance to various inhibitive compounds and pH changes. [ 14,15 ] In addition, C. beijerinckii does not have obvious carbon catabolite repression effect, [ 16,17 ] which has been observed for many other solventogenic clostridia such as the industrial strain C. acetobutylicum . [ 18 ] This feature makes C. beijerinckii suitable for utilizing complex biomass for organic solvent production.…”

Section: Introductionmentioning

confidence: 99%

The SCIFF‐Derived Ranthipeptides Participate in Quorum Sensing in Solventogenic Clostridia

Chen

Yang

et al. 2020

Biotechnology Journal

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Ranthipeptides, defined as radical non-thioether-containing peptides, are a newly emerging class of natural products belonging to the ribosomally synthesized and post-translationally modified peptide (RiPP) superfamily. Ranthipeptides are shown to be widespread in the bacterial kingdom, whereas heretofore their biological functions remain completely elusive. In this work, putative ranthipeptides are investigated from two solventogenic clostridia, Clostridium beijerinckii and Clostridium ljungdahlii, which are derived from the so-called six Cys in forty-five residues (SCIFF) family of precursor peptides. A series of analysis show that these two ranthipeptides participate in quorum sensing and controlling cellular metabolism. These results highlight the diverse biological functions of the ever-increasing family of RiPP natural products and showcase the potential to engineer industrially interesting organisms by manipulating their RiPP biosynthetic pathways.

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The advanced strategy for enhancing biobutanol production and high-efficient product recovery with reduced wastewater generation

Cited by 38 publications

References 45 publications

Effect of overliming and activated carbon detoxification on inhibitors removal and butanol fermentation of poplar prehydrolysates

Effect of overliming and activated carbon detoxification on inhibitors removal and butanol fermentation of poplar prehydrolysates

Biobutanol production from coffee silverskin

The SCIFF‐Derived Ranthipeptides Participate in Quorum Sensing in Solventogenic Clostridia

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