The Advanced Lipoxidation End-Product Malondialdehyde-Lysine in Aging and Longevity

Jové, Mariona; Mota‐Martorell, Natàlia; Pradas, Irene; Martín-Gari, Meritxell; Ayala, Victòria; Pamplona, Reinald

doi:10.3390/antiox9111132

Cited by 57 publications

(51 citation statements)

References 140 publications

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“…This was reported in cows [ 63 ] and lysine-fed ewes (L, LML, HML) of our study. In vitro studies reported that although MDA can react with a broad variety of AA, the epsilon amino group of lysine is the main target [ 64 , 65 ]. Additionally, proteins compared to the free amino acids are more easily damaged by MDA under physiological conditions, as has been indicated in vitro [ 64 , 66 ].…”

Section: Discussionmentioning

confidence: 99%

Effects of Supplementing Rumen-Protected Methionine and Lysine on Milk Performance and Oxidative Status of Dairy Ewes

et al. 2021

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There is limited information on the impact of dietary supplementation with separate rumen-protected (RP) amino acids (AA), or with their combination, on ewes’ oxidative status. Sixty ewes were divided into five groups; C: basal diet (control); M: basal diet + 6 g/ewe RP methionine; L: basal diet + 5 g/ewe RP lysine; LML: basal diet + 6 g methionine and 5 g lysine/ewe; and HML: basal diet + 12 g methionine + 5 g lysine/ewe. Milk’s fat content increased in RP-AA fed ewes, while that of protein in M and L only. In blood plasma, the malondialdehyde (MDA) content was reduced in the M, LML, and HML compared to C-fed ewes. An increase in glutathione transferase activity in the blood plasma of the M and LML compared to the C and HML-fed ewes were found. In milk, lower values of the ferric reducing ability of plasma (FRAP) in the LML and HML-fed ewes and of 2,2′-Azino-bis 3-ethylbenzthiazoline-6-sulfonic acid (ABTS) in LML only, were found. Lysine increased milk’s FRAP values and MDA content. Both L and HML diets increased milk’s protein carbonyls content. Methionine improves the organism’s oxidative status, without adversely affecting milk’s oxidative stability. Lysine dietary inclusion affects negatively the oxidative stability of milk.

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Section: Discussionmentioning

confidence: 99%

Effects of Supplementing Rumen-Protected Methionine and Lysine on Milk Performance and Oxidative Status of Dairy Ewes

et al. 2021

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“…Although this review is more focused on lipoxidation in the cellular context, protein lipoxidation in the extracellular milieu and the bloodstream has important consequences, including increased immunogenicity, transfer of proinflammatory and damage signals and contribution to a variety of pathophysiological processes [ 12 , 127 ]. In summary, lipoxidation can impact essential processes including cell signalling and metabolism, cytoskeletal function, protein degradation and gene expression.…”

Section: Functional Consequences Of Lipoxidationmentioning

confidence: 99%

Protein Lipoxidation: Basic Concepts and Emerging Roles

et al. 2021

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Protein lipoxidation is a non-enzymatic post-translational modification that consists of the covalent addition of reactive lipid species to proteins. This occurs under basal conditions but increases in situations associated with oxidative stress. Protein targets for lipoxidation include metabolic and signalling enzymes, cytoskeletal proteins, and transcription factors, among others. There is strong evidence for the involvement of protein lipoxidation in disease, including atherosclerosis, neurodegeneration, and cancer. Nevertheless, the involvement of lipoxidation in cellular regulatory mechanisms is less understood. Here we review basic aspects of protein lipoxidation and discuss several features that could support its role in cell signalling, including its selectivity, reversibility, and possibilities for regulation at the levels of the generation and/or detoxification of reactive lipids. Moreover, given the great structural variety of electrophilic lipid species, protein lipoxidation can contribute to the generation of multiple structurally and functionally diverse protein species. Finally, the nature of the lipoxidised proteins and residues provides a frameshift for a complex interplay with other post-translational modifications, including redox and redox-regulated modifications, such as oxidative modifications and phosphorylation, thus strengthening the importance of detailed knowledge of this process.

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“…The content of MDA can reflect the level of lipid peroxidation and the damage degree of cell lipid peroxidation to some extent. 43 Antioxidant enzymes SOD, CAT, and GSH-Px are the primary substances for scavenging free radicals in organisms, and their levels in organisms indicate an intuitive index of oxidative damage rate. 44 Most studies have shown that antioxidants are an important step in preventing aging because the build-up of ROS can lead to DNA damage, which has been shown to have a significant impact on aging.…”

Section: Discussionmentioning

confidence: 99%

Exploring the Protective and Reparative Mechanisms of G. lucidum Polysaccharides Against H2O2-Induced Oxidative Stress in Human Skin Fibroblasts

Shi¹,

Cheng²,

Wang³

et al. 2021

CCID

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Background: Ganoderma lucidum (G. lucidum) is one of China's traditional medicinal materials. G. lucidum polysaccharide has a wide range of promising pharmacological applications. However, there are many kinds of G. lucidum and they contain different kinds of polysaccharides. The biological mechanism through which Ganoderma lucidum polysaccharides (GLP) is able to protect human skin fibroblasts (HSFs) from H 2 O 2 -induced oxidative damage is still unclear. Methods: Six polysaccharides were obtained from G. lucidum to evaluate their free radical scavenging ability (DPPH free radical, ABTS free radical, hydroxyl-free radical, superoxide anion-free radical) in vitro, and their protective and reparative effects on oxidative damage induced by H 2 O 2 in human skin fibroblasts. One polysaccharide was selected to detect oxidative damage markers and gene expression in the Keap1-Nrf2/ARE signaling pathway in HSFs. Results: All six polysaccharides showed the ability to scavenge free radicals and enhance the tolerance of human skin fibroblasts to H2O2 damage. Among them, GLP1 was selected and separated into two components (GLP1I and GLP1II). The results showed that GLP1, GLP1I and GLPII could significantly reduce the levels of reactive oxygen species (ROS) and malondialdehyde (MDA). The protective effect of GLP1II was stronger than that of positive control vitamin C. In addition, GLP1, GLP1I and GLP1II could significantly increase the levels of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-Px). And GLP1I works best in both ways. Meanwhile, Nrf2, a key regulator of keAP1-NRF2/ARE signaling pathway, was activated, while Keap1, a negative regulator, was inhibited, thus promoting the expression of downstream antioxidant enzyme genes (GSTs, GCLs, Nqo1, and Ho-1). Conclusion:The results showed that GLP could protect human skin fibroblasts from oxidative damage caused by H 2 O 2 peroxide by enhancing enzyme activity and activating Keap1-Nrf2/ARE signaling pathway. GLP will act as a natural antioxidant to protect the skin from oxidative stress damage.

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The Advanced Lipoxidation End-Product Malondialdehyde-Lysine in Aging and Longevity

Cited by 57 publications

References 140 publications

Effects of Supplementing Rumen-Protected Methionine and Lysine on Milk Performance and Oxidative Status of Dairy Ewes

Effects of Supplementing Rumen-Protected Methionine and Lysine on Milk Performance and Oxidative Status of Dairy Ewes

Protein Lipoxidation: Basic Concepts and Emerging Roles

Exploring the Protective and Reparative Mechanisms of G. lucidum Polysaccharides Against H2O2-Induced Oxidative Stress in Human Skin Fibroblasts

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