The Adult Drosophila Malpighian Tubules Are Maintained by Multipotent Stem Cells

Singh, Shree Ram; Liu, Wei; Hou, Steven X.

doi:10.1016/j.stem.2007.07.003

Cited by 166 publications

(100 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Besides identification of signals and mechanisms involved in stem cell maintenance and differentiation, these studies have also revealed mechanisms underlying stem cell and niche interaction, essential for maintenance of healthy stem cell populations (Losick et al, 2011; Pearson et al, 2009; Hsu et al, 2014; Gunage et al, 2014; Yuan and Yamashita, 2010; Lin, 2002; Singh et al, 2007; Inaba et al, 2015). …”

Section: Introductionmentioning

confidence: 99%

Dpp dependent Hematopoietic stem cells give rise to Hh dependent blood progenitors in larval lymph gland of Drosophila

Dey

Ramesh

Chugh

et al. 2016

eLife

View full text Add to dashboard Cite

Drosophila hematopoiesis bears striking resemblance with that of vertebrates, both in the context of distinct phases and the signaling molecules. Even though, there has been no evidence of Hematopoietic stem cells (HSCs) in Drosophila, the larval lymph gland with its Hedgehog dependent progenitors served as an invertebrate model of progenitor biology. Employing lineage-tracing analyses, we have now identified Notch expressing HSCs in the first instar larval lymph gland. Our studies clearly establish the hierarchical relationship between Notch expressing HSCs and the previously described Domeless expressing progenitors. These HSCs require Decapentapelagic (Dpp) signal from the hematopoietic niche for their maintenance in an identical manner to vertebrate aorta-gonadal-mesonephros (AGM) HSCs. Thus, this study not only extends the conservation across these divergent taxa, but also provides a new model that can be exploited to gain better insight into the AGM related Hematopoietic stem cells (HSCs).DOI: http://dx.doi.org/10.7554/eLife.18295.001

show abstract

Section: Introductionmentioning

confidence: 99%

Dpp dependent Hematopoietic stem cells give rise to Hh dependent blood progenitors in larval lymph gland of Drosophila

Dey

Ramesh

Chugh

et al. 2016

eLife

View full text Add to dashboard Cite

show abstract

“…Drosophila hemocytes were fixed and stained as previously described (Lanot et al, 2001). The following primary antibodies were used: mouse anti-Dorsal (7A4, 1:5, DSHB), rabbit anti-DIF (Rutschmann et al, 2000) (1:100), rabbit anti-phosphoHistone H3 (Singh et al, 2007) (1:500), mouse anti-Cut (2B10, 1:5, DSHB), rat anti-DE-cad (DCAD2, 1:2.5, DSHB) and mouse anti-Antp (8C11, 1:5, DSHB). Alexa Fluor 488 (Molecular Probes), Cy3 and Cy5 (Jackson Immuno)-conjugated secondary antibodies were used at a dilution of 1:400.…”

Section: Methodsmentioning

confidence: 99%

Retromer Promotes Immune Quiescence by Suppressing Spätzle‐Toll Pathway in Drosophila

Zhou

Yun

Ray

et al. 2013

Journal Cellular Physiology

View full text Add to dashboard Cite

The Toll and Toll-Like Receptor signaling pathways are evolutionarily conserved pathways that regulate innate immunity in insects and mammals. While efforts have been made to clarify the signal transduction events that occur during infection, much less is known about the components that maintain immune quiescence. Here we show that retromer, an intracellular protein complex known for regulating vesicle trafficking, functions in modulating the Toll pathway in Drosophila melanogaster. In mutant animals lacking retromer function, the Toll pathway but not JAK-STAT or IMD pathway is activated, triggering both cellular and humoral responses. Genetic epistasis and clonal analysis suggest that retromer regulates a component that acts upstream of Toll. Our data further show that in the mutant the Toll ligand Spätzle has a processing pattern similar to that of after infection. Together, the results suggest a novel function of retromer in regulating Toll pathway and innate immunity at a step that modulates ligand processing or activity.

show abstract

“…In Drosophila, both the gut (Jiang et al, 2011; Micchelli and Perrimon, 2006; Ohlstein and Spradling, 2006) and Malphigian tubules (equivalent to kidney nephrons) (Singh et al, 2007)are maintained by stem cell division and cell turnover, however it has not yet been determined if the rate of cell turnover is important for normal longevity. Over-expression of caspase inhibitors p35 and DIAP1 in adult flies did not affect life span, suggesting that adult fly life span is not limited by a canonical caspase-dependent PCD(Shen et al, 2009), however a possible role for caspase-independent PCD or necrosis pathways in regulating adult fly life span cannot yet be ruled out.…”

Section: Pcd In Life Span Regulationmentioning

confidence: 99%

Programmed cell death in aging

Tower

2015

Ageing Research Reviews

313

208

View full text Add to dashboard Cite

Programmed cell death (PCD) pathways, including apoptosis and regulated necrosis, are required for normal cell turnover and tissue homeostasis. Mis-regulation of PCD is increasingly implicated in aging and aging-related disease. During aging the cell turnover rate declines for several highly-mitotic tissues. Aging-associated disruptions in systemic and inter-cell signaling combined with cell-autonomous damage and mitochondrial malfunction result in increased PCD in some cell types, and decreased PCD in other cell types. Increased PCD during aging is implicated in immune system decline, skeletal muscle wasting (sarcopenia), loss of cells in the heart, and neurodegenerative disease. In contrast, cancer cells and senescent cells are resistant to PCD, enabling them to increase in abundance during aging. PCD pathways limit life span in fungi, but whether PCD pathways normally limit adult metazoan life span is not yet clear. PCD is regulated by a balance of negative and positive factors, including the mitochondria, which are particularly subject to aging-associated malfunction.

show abstract

The Adult Drosophila Malpighian Tubules Are Maintained by Multipotent Stem Cells

Cited by 166 publications

References 41 publications

Dpp dependent Hematopoietic stem cells give rise to Hh dependent blood progenitors in larval lymph gland of Drosophila

Dpp dependent Hematopoietic stem cells give rise to Hh dependent blood progenitors in larval lymph gland of Drosophila

Retromer Promotes Immune Quiescence by Suppressing Spätzle‐Toll Pathway in Drosophila

Programmed cell death in aging

Contact Info

Product

Resources

About