The adsorption of divalent cations to phosphatidylcholine bilayer membranes

McLaughlin, Alan C.; Grathwohl, Christoph; McLaughlin, Stuart

doi:10.1016/0005-2736(78)90203-1

Cited by 220 publications

(123 citation statements)

References 57 publications

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“…Our results for POPC generally agree with previous work indicating that POPC is neutral (25)(26)(27)(28). The observation that the POPC Nanodisc charge was unaffected by the monovalent alkali cation type (Fig.…”

Section: Polyelectrolyte Behavior Of Lipid Nanodiscssupporting

confidence: 83%

“…For POPC Nanodiscs, the total charge measured on the Nanodisc can be largely accounted for by the MSPs, with the POPC itself being neutral and thus contributing minimally to the measured charge. This observation is in general agreement with previous electrophoretic mobility measurements (25)(26)(27) showing that POPC liposomes, stearoyl-oleoylphosphatidylcholine liposomes, and egg phosphatidylcholine (PC) lipid vesicles have little to no charge. Therefore, assuming that the protein charge remains constant with the addition of lipids, and that POPC lipids contribute a net charge of zero, the MSP contribution to the Z DHH of a Nanodisc can be calculated as…”

Section: Popc Is a Neutral Lipid But Pope Is Anionicsupporting

confidence: 82%

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The Charge Properties of Phospholipid Nanodiscs

Her

Filoti

McLean

et al. 2016

Biophysical Journal

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Phospholipids (PLs) are a major, diverse constituent of cell membranes. PL diversity arises from the nature of the fatty acid chains, as well as the headgroup structure. The headgroup charge is thought to contribute to both the strength and specificity of protein-membrane interactions. Because it has been difficult to measure membrane charge, ascertaining the role charge plays in these interactions has been challenging. Presented here are charge measurements on lipid Nanodiscs at 20 C in 100 mM NaCl, 50 mM Tris, at pH 7.4. Values are also reported for measurements made in the presence of Ca 2þand Mg 2þ as a function of NaCl concentration, pH, and temperature, and in solvents containing other types of cations and anions. Measurements were made for neutral (phosphatidylcholine and phosphatidylethanolamine) and anionic (phosphatidylserine, phosphatidic acid, cardiolipin, and phosphatidylinositol 4,5-bisphosphate (PIP 2 )) PLs containing palmitoyl-oleoyl and dimyristoyl fatty acid chains. In addition, charge measurements were made on Nanodiscs containing an Escherichia coli lipid extract. The data collected reveal that 1) POPE is anionic and not neutral at pH 7.4; 2) high-anionic-content Nanodiscs exhibit polyelectrolyte behavior; 3) 3 mM Ca 2þ neutralizes a constant fraction of the charge, but not a constant amount of charge, for POPS and POPC Nanodiscs; 4) in contrast to some previous work, POPC only interacts weakly with Ca 2þ ; 5) divalent cations interact with lipids in a lipid-and ion-specific manner for POPA and PIP 2 lipids; and 6) the monovalent anion type has little influence on the lipid charge. These results should help eliminate inconsistencies among data obtained using different techniques, membrane systems, and experimental conditions, and they provide foundational data for developing an accurate view of membranes and membrane-protein interactions.

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Section: Polyelectrolyte Behavior Of Lipid Nanodiscssupporting

confidence: 83%

Section: Popc Is a Neutral Lipid But Pope Is Anionicsupporting

confidence: 82%

The Charge Properties of Phospholipid Nanodiscs

Her

Filoti

McLean

et al. 2016

Biophysical Journal

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“…Like monovalent cations, bivalent cations strongly bind to acidic phospholipids [35,31,57,[59][60][61][62]. Cations have also been shown to interact with phosphatidylcholine, a zwitterionic lipid [49, 65,661.…”

Section: Discussionmentioning

confidence: 99%

Effect of pH and Monovalent Cations on the Ionization State of Phosphatidylglycerol in Monolayers

Lakhdar-Ghazal

Tichadou

Tocanne

1983

European Journal of Biochemistry

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Ionization of the acidic phospholipid phosphatidylglycerol has been studied by measuring the surface potential of monomolecular films of the lipid as a function of the aqueous subphase pH and the concentration of monovalent cations (Li, Na, Cs). It is shown that theexperimental data can be interpreted by means of the Gouy-Chapman theory in its simplest formulation, provided an adsorption of cations at the membrane surface is accounted for. This allows us to predict the ionization state of the lipid for given ionic conditions in the subphase. Above pH 4, for subphase ion concentration higher than 10 mM, or for ion concentrations above 0.1 mM at pH 5.6, phosphatidylglycerol is fully deprotonated. Within the limits of our theoretical approach, association constants of the cations to the lipid lie around 0.1-0.6 M-I.With the exception of phosphatidylcholine and phosphatidylethanolamine which are zwitterions over a large pH range (3 -8) [l], phospholipids in biomembranes are acidic substances bearing a net negative charge when they are ionized. Ionization or protonation of these acidic lipids as well as their interactions with cations can have considerable consequences on both membrane structure and functions.From a structural point of view, changes in the pH or the ionic strength of the aqueous phase have been shown to trigger phase transition of acidic phospholipids [2-111. The bivalent cations Mg2+ and Ca2+ can bring about phase separation within mixtures of zwitterionic and acidic phospholipids [12-191. Cations can also modify the dynamic properties of lipids, i.e. their lateral diffusion rate [20], as well as being able to promote morphological changes [21]. As an example, cardiolipin, which normally exists in a lamellar phase in the presence of sodium, adopts the hexagonal H,, phase in the presence of calcium [22, 231. Dipalmitoylglycerophosphoglycerol, which normally stays in a lamellar phase in the presence of sodium, has been shown to exist in an interdigitated phase in the presence of the organic cations choline and acetylcholine [24].From a functional point of view, phospholipids can modulate the activity of membrane enzymes by monitoring, at the membrane surface, a concentration of protons, cations and of any charged metabolites which is quite different from that existing in the bulk [25]. Charges at the membrane surface also contribute to the establishment of transmembrane potentials which are involved in energy-linked transmembrane transport processes [26] and which are postulated to monitor the orientation [27 -291 and the activity of certain membrane proteins [28 -341.For all these reasons, it is of great importance to have a full understanding of the ionization process of acidic phospholipids in membrane systems. This would allow a correct prediction of their ionization state and of the surface potential they generate, for given ionic conditions in the aqueous phase. As a first approach, ionization of phosphatidylglycerol was studied in monolayers by measuring changes in film surface pressure again...

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“…The influence of physiologically relevant ions (Na + , K + , Cl − , Ca 2+ , and Mg 2+ ) on model lipid membranes was extensively studied in the past (Böckmann et al 2003, Vacha et al 2009 (McLaughlin et al 1978, Garcia-Celma et al 2007), but, because the specificity of ionic effects were more pronounced for anions than for cations, the number of experimental data on analysis of cationic effects were sparser (Tatulian 1987, Clarke and Lupfert 1999, Kunz et al 2004, Garcia-Celma et al 2007). However, the conclusion drawn from all these studies was unequivocal: the strongest effects were observed for ions characterized by a high charge density: bivalent cations Mg 2+ , Ca 2+ and Li + (Pabst et al 2007, General discussion 2013.…”

Section: Lithium and Membrane Lipids; Biophysical Studies Of Direct Imentioning

confidence: 99%

Lithium – therapeutic tool endowed with multiple beneficiary effects caused by multiple mechanisms

Vošahlíková¹,

Svoboda²

2016

Acta Neurobiologiae Experimentalis

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Mood disorders are relatively common serious human diseases for which there is often no ideal pharmacotherapy. Basic characteristic of these diseases is affective disorder shifting the mood of the patient to depression (together with anxiety or not) or towards to euphoria. Available drugs are usually divided into two groups -mood stabilizers, which are used primarily to treat bipolar disorder, and antidepressants for the treatment of unipolar depression. Lithium is still recommended as the first choice for dealing with bipolar disorder. Despite abundant clinical use of mood stabilizing drugs, important questions regarding their mechanism of action remain open.In this paper we present the brief review of rather diversified hypotheses and ideas about mechanisms of genesis of mood disorders and lithium interferences with these pathological states. New data derived from the high-resolution crystallographic studies of allosteric,

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The adsorption of divalent cations to phosphatidylcholine bilayer membranes

Cited by 220 publications

References 57 publications

The Charge Properties of Phospholipid Nanodiscs

The Charge Properties of Phospholipid Nanodiscs

Effect of pH and Monovalent Cations on the Ionization State of Phosphatidylglycerol in Monolayers

Lithium – therapeutic tool endowed with multiple beneficiary effects caused by multiple mechanisms

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