The Administration of Jeungmiyijin-tang to Rats with Induced Gastro Reflux Esophagitis

Abstract

Objectives: This study investigated the administration of Jeungmiyijin-tang (JYT) to rats with reflux esophagitis (RE) induced by pylorus and forestomach ligation operations. Methods: Twenty laboratory rats were divided into three groups with 5~7 rats in each group. The control group consisted of rats with no inflammation (CON). The RE group had rats with gastroesophageal reflux elicited by pylorus and forestomach ligation operations. The JYT group had rats that were orally administered Jeungmiyijin-tang (1.5 ml/day/300 g) once a day for 14 days before reflux esophagitis was induced by the pylorus and forestomach ligation operations. Six hours after the operations, the rats were sacrificed, morphological changes were observed, and histological examinations were done in the stomach and esophagus lesion areas. If apoptosis was observed, the apoptotic cells in the esophagus lesion areas were counted. Results: The morphological and histochemical changes consisted of various injuries from hemorrhagic erosion in the RE group, while there were significantly fewer in the JYT group. The RE group marked increases of gastric mucosa erosion and infiltration of inflammatory cells in the submucosa, as well as cell division in the epithelial layer, the proliferation and degranulation of mast cells, and increases in the IL-1β, TNF-α, and MMP-9 expressions in the esophagus of the rats. The JYT group was inhibited above expression compared with the RE group. Apoptosis was statistically significantly decreased in the JYT group compared with the RE group. Conclusions: According to the above results, it appears that Jeungmiyijin-tang inhibits the expression of pro-inflammatory cytokines (TNF-a, IL-1β, and MMP-9) and apoptosis in the esophagus mucosa, thereby preventing esophageal mucosal damage from esophageal reflux.

Effectiveness analysis of herbal medicine for gastroesophageal reflux disease: A retrospective study

“…Notably, these 4 herbs are key components of Yijin-tang, a traditional herbal formula that has been used for centuries to address GERD and has demonstrated effectiveness in various animal models of GERD. [19,20] A previous study conducted in China also reported superior therapeutic outcomes for this herbal formula compared to omeprazole magnesium enteric-coated tablets in patients with GERD. [21] These findings align precisely with a previous review of Yijintang for GERD based on classical herbal literature.…”

“…The study’s findings suggested that this mechanism offers protection against reflux-induced mucosal damage. [ 20 ] In addition, another case study showed that Jeungmiyijin-tang plus Soojeom-san almost resolved a patient’s GERD symptoms. [ 34 ] Yukul-tang , in a rat experiment, demonstrated a significant reduction in the incidence of gastric ulcers compared to the control group.…”

Regulation of Gastric Acid Secretion of Liriope platyphylla Extract in Gastroesophageal Reflux Disease

“…Concerning GERD and reflux esophagitis, there were studies on a variety of prescriptions and single or complex medications such as (597) Banhasasim-tang 13) , Yijin-tang 14,15) , Jwakum-whan 16) , Coptis Chinensis 17) , Artemisia Capillaris Herba 18) , Evodiae Fructus 19,20) , Citri Pericarpium 21) , and Ulmus Pumila Cortex 22) . However, there was no study comparing the therapeutic effect of Liriope platyphylla with PPI in studies related to GERD and reflux esophagitis.…”