The adherence of Candida yeasts to human and bovine vascular endothelium and subendothelial extracellular matrix

Klotz, Stephen A.

doi:10.1016/0378-1097(87)90162-5

Cited by 11 publications

(23 citation statements)

References 5 publications

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“…24-48 h before use in binding experiments, spirochetes were subcultured in medium containing [10][11][12][13][14][15] ,uCi/ml [3H]thymidine (60-74 Ci/mmol; ICN Radiochemicals, Irvine, CA). Spirochetes were pelleted at 7,000 g for 15 min at 20°C, washed three times in HBSS, and resuspended in RPMI 1640 containing 10 mg/ml bovine serum albumin and 10 mM Hepes (Gibco Laboratories, Grand Island, NY) (RBH).'…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Interaction between Borrelia burgdorferi and endothelium in vitro.

Szczepanski¹,

Furie²,

Benach³

et al. 1990

J. Clin. Invest.

177

146

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During the pathogenesis of Lyme disease, Borrelia burgdorferi spreads hematogenously from the site of a tick bite to several tissues throughout the body. The specific mechanism of spirochete emigration is presently unknown. Using cultured human umbilical vein endothelial cells, we found that Borrelia burgdorfen bound to the endothelial cells and to the subendothelial matrix. Low passage isolates adhered 22-30-fold greater than a strain maintained in culture continuously. Spirochete binding to subendothelial matrix was inhibited 48-63% by pretreatment of the matrix with anti-fibronectin antiserum. Spirochete migration across endothelial monolayers cultured on amniotic membrane was increased when the monolayers were damaged by chemical or physical means. Electron microscopic examination of spirochete-endothelial interactions demonstrated the presence of spirochetes in the intercellular junctions between endothelial cells as well as beneath the monolayers. Scanning electron microscopy identified a mechanism of transendothelial migration whereby spirochetes pass between cells into the amniotic membrane at areas where subendothelium is exposed.

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Section: Methodsmentioning

confidence: 99%

“…Collagenase (CLSII; Worthington, Freehold, NJ) at a final concentration of 0.03% in HBS was infused into the vein. The cord was then incubated for 12 min at 370C. The collagenase solution was recovered from the vein and the vein was washed once with 25 ml of M 199 containing 5% heat-inactivated donor calf serum.…”

Section: Methodsmentioning

confidence: 99%

Interaction between Borrelia burgdorferi and endothelium in vitro.

Szczepanski¹,

Furie²,

Benach³

et al. 1990

J. Clin. Invest.

177

146

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“…C. albicans and C. tropicalis, which are often isolated from infections, bound significantly better to fibronectin in vitro than the infrequently isolated C. krusei. During the analysis of adherence to endothelial monolayers, it was noted that C. albicans adhered preferentially to subendothelial ECM (Klotz, 1987;Klotz and Maca, 1988). The candidal binding proteins for iC3b and fibronectin are discussed below.…”

Section: Removalmentioning

confidence: 99%

Oral Colonization By Candida Albicans

Cannon

Chaffin

1999

Critical Reviews in Oral Biology & Medicine

276

193

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Candida albicans is a commensal yeast normally present in small numbers in the oral flora of a large proportion of humans. Colonization of the oral cavity by C. albicans involves the acquisition and maintenance of a stable yeast population. Micro-organisms are continually being removed from the oral cavity by host clearance mechanisms, and so, in order to survive and inhabit this eco-system, C. albicans cells have to adhere and replicate. The oral cavity presents many niches for C. albicans colonization, and the yeast is able to adhere to a plethora of ligands. These include epithelial and bacterial cell-surface molecules, extracellular matrix proteins, and dental acrylic. In addition, saliva molecules, including basic proline-rich proteins, adsorbed to many oral surfaces promote C. albicans adherence. Several adhesins present in the C. albicans cell wall have now been partially characterized. Adherence involves lectin, protein-protein, and hydrophobic interactions. As C. albicans cells evade host defenses and colonize new environments by penetrating tissues, they are exposed to new adherence receptors and respond by expressing alternative adhesins. The relatively small number of commensal Candida cells in the oral flora raises the possibility that strategies can be devised to prevent oral colonization and infection. However, the variety of oral niches and the complex adherence mechanisms of the yeast mean that such a goal will remain elusive until more is known about the contribution of each mechanism to colonization.

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“…Not surprisingly, when one considers the incidence of various Candida species as etiologic agents of disease, C. albicans is significantly more adherent to buccal, vaginal, or HeLa cells (5,21,57); to skin corneocytes (85); to fibrin-platelet matrices (67); or to fibronectin (16,63). Tissue specificity may also play a role in that adhesion mechanisms and exposed ligands appear to vary among different types of epithelial cells and between epithelial and endothelial cells.…”

Section: Clinical Relevancementioning

confidence: 99%

“…Studies of the preferential adhesion of C. albicans and C tropicalis to subendothelial matrix proteins, as opposed to endothelium itself, were made after intriguing initial observations (63). C. albicans and C. tropicalis adhered more avidly to the contracted monolayer, which exposed subendothelial extracellular matrix, than to the confluent monolayer of bovine aortic endothelial cells (60).…”

Section: Protein-protein Interactionsmentioning

confidence: 99%

Adhesins and ligands involved in the interaction of Candida spp. with epithelial and endothelial surfaces

1994

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Adhesion of candidal species to the epithelium of the gastrointestinal or genitourinary tract stands as a critical first step in the pathogenesis of candidal infection. After colonization and replication at mucosal surfaces, Candida albicans and other pathogenic species may penetrate the mucosal barrier, enter the vascular tree, and disseminate hematogenously. The consequences of this pathogenic cascade evoke considerable morbidity and mortality, especially among immunocompromised patients. Thus, interactions of C. albicans and other candidal species with epithelium and endothelium may lead to serious consequences for the human host. This review evaluates candidate candidal adhesions for epithelial and endothelial surfaces, with emphasis on the specificity of the interaction, the inhibitors that have been employed, and the ligands that have been identified on mammalian cells or matrices. Three types of interactions are described: protein-protein interactions, lectin-like interactions, and incompletely defined interactions in which the adhesive ligand is as yet unidentified. Special attention is given to the roles of integrin-like proteins. Differences in the mechanisms of candidal attachment to epithelium and endothelium are delineated. Last, on the basis of the available literature, avenues of potentially fruitful investigation are proposed.

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The adherence of Candida yeasts to human and bovine vascular endothelium and subendothelial extracellular matrix

Cited by 11 publications

References 5 publications

Interaction between Borrelia burgdorferi and endothelium in vitro.

Interaction between Borrelia burgdorferi and endothelium in vitro.

Oral Colonization By Candida Albicans

Adhesins and ligands involved in the interaction of Candida spp. with epithelial and endothelial surfaces

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