The adenosinergic system, not the cholinergic system, exerts an anti-inflammatory profile in lymphatic immune organs of fish naturally infected with Ichthyophthirius multifiliis

Baldissera, Matheus D.; Souza, Carine F.; Doleski, Pedro H.; Moreira, Karen L.S.; Veiga, Marcelo Leite da; Rocha, Maria Izabel de Ugalde Marques da; Almeida, Ana Paula Gottlieb; Baldisserotto, Bernardo

doi:10.1016/j.aquaculture.2017.04.017

Cited by 9 publications

(6 citation statements)

References 32 publications

(21 reference statements)

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“…An average of 124 ± 21 trophonts were observed in naturally infected fish with I. multifiliis after 4 days of laboratory observation, as previously published by Baldissera et al. ().…”

Section: Methodssupporting

confidence: 81%

“…Seric nitrite/nitrate content was measured spectrophotometrically by the Griess reaction, as described previously in detail by Baldissera et al. (). The results were expressed as μmol/L.…”

Section: Methodsmentioning

confidence: 99%

“…The pH was measured using a DMPH-2 pH meter (Digimed, Sao Paulo, Brazil). Total ammonia levels were determined according to Verdouw, Vanechteld, and Deckkers (1978), and non-ionized ammonia (NH 3 ) levels were calculated according to Colt (2001), as recently published in detail by Baldissera et al (2017). The animals were fed to apparent satiation with commercial feed once daily.…”

Section: Fish Harvesting Maintenance and Water Quality Variablesmentioning

confidence: 99%

“…ADA activity was measured at 650 nm, and the specificity activity was reported as U/L. Seric Ado levels were measured with an Ado assay kit (Biovision Inc., CA), according to the manufacturer's protocol, and as recently published in detail by Baldissera et al (2017). The fluorescence was measured using excitation and emission wavelengths of 535 and 587, respectively, using a fluorescence spectrophotometer.…”

Section: Ada Activity and Ado Levelsmentioning

confidence: 99%

“…The seric ROS levels were determined by the DCFH oxidation method described by Keston and Brandt (1965), recently published in detail by Baldissera et al (2017). Fluorescence was measured using excitation and emission wavelengths of 485 and 538 nm, respectively.…”

Section: Ros and Nox Levelsmentioning

confidence: 99%

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Serum adenosine deaminase and xanthine oxidase activities in silver catfish naturally infected with Ichthyophthirius multifiliis: The influence of these enzymes on inflammatory and oxidative status

Baldissera

Souza

Doleski

et al. 2017

Journal of Fish Diseases

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Adenosine deaminase (ADA) activity, through adenosine (Ado) levels, as well as xanthine oxidase (XO) activity through uric acid levels exerts an essential role on immune and inflammatory responses during infectious diseases. Thus, the aim of this study was to evaluate the involvement of seric ADA and XO activities in the inflammatory and oxidative status of silver catfish naturally infected with Ichthyophthirius multifiliis. Seric ADA activity decreased, while Ado levels increased in infected animals compared to uninfected animals. Moreover, the seric XO activity increased in infected animals compared to uninfected animals, alongside the seric levels of uric acid, metabolites of nitric oxide (NOx) and reactive oxygen species (ROS). Based on this evidence, the downregulation of seric ADA activity exerts an anti-inflammatory profile, contributing to restricting the inflammatory process. The most important finding is that upregulation of seric XO activity leads to an excessive formation of uric acid, which contributes to oxidative and inflammatory processes. Moreover, uric acid induces the release of pro-inflammatory and pro-oxidative mediators, such NOx and ROS, which contribute directly to disease pathogenesis. In summary, the upregulation of XO activity may be considered a pathway involved in NOx and ROS production in silver catfish infected with I. multifiliis.

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“…An average of 124 ± 21 trophonts were observed in naturally infected fish with I. multifiliis after 4 days of laboratory observation, as previously published by Baldissera et al. ().…”

Section: Methodssupporting

confidence: 81%

“…Seric nitrite/nitrate content was measured spectrophotometrically by the Griess reaction, as described previously in detail by Baldissera et al. (). The results were expressed as μmol/L.…”

Section: Methodsmentioning

confidence: 99%

Section: Fish Harvesting Maintenance and Water Quality Variablesmentioning

confidence: 99%

Section: Ada Activity and Ado Levelsmentioning

confidence: 99%

Section: Ros and Nox Levelsmentioning

confidence: 99%

See 3 more Smart Citations

Serum adenosine deaminase and xanthine oxidase activities in silver catfish naturally infected with Ichthyophthirius multifiliis: The influence of these enzymes on inflammatory and oxidative status

Baldissera

Souza

Doleski

et al. 2017

Journal of Fish Diseases

Self Cite

View full text Add to dashboard Cite

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Ichthyophthirius multifiliis impairs splenic enzymes of the phosphoryl transfer network in naturally infected Rhamdia quelen: effects on energetic homeostasis

2017

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Its integrated energetic and metabolic signaling roles place the phosphoryl transfer network, through the enzymes creatine kinase (CK), adenylate kinase (AK), and pyruvate kinase (PK), as a regulatory system coordinating components of the cellular bioenergetics network. Analysis of these enzymes provides new information and perspectives with which to understand disturbances in energetic metabolism between sites of adenosine triphosphate (ATP) generation and utilization. Thus, the aim of this study was to evaluate the involvement of the phosphoryl transfer network in splenic tissue linked with the pathogenesis of silver catfish naturally infected with Ichthyophthirius multifiliis. Splenic cytosolic and mitochondrial CK activities decreased in infected animals compared to uninfected animals, as was also observed for splenic PK activity and splenic ATP levels. In contrast, splenic AK activity increased in infected animals compared to uninfected animals. Based on this evidence, the inhibition and absence of efficient communication between CK isoenzymes cause the impairment of splenic bioenergetics, which is in turn compensated by the augmentation of splenic AK activity in an attempt to restore energy homeostasis. The inhibition of splenic PK activity impairs communication between sites of ATP generation and ATP utilization, as corroborated by splenic ATP depletion. In summary, these alterations contribute to disease pathogenesis linked to spleen tissue in animals infected with white spot disease.

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Purinergic signaling modulates the splenic inflammatory response in silver catfish naturally infected with Ichthyophthirius multifiliis

2018

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The spleen is an immune lymphatic organ linked with control of the immune response, which is important to fish health. Recent evidence has suggested the involvement of purinergic signaling in the modulation of immune and inflammatory responses through the nucleotide adenosine triphosphate (ATP) and the nucleoside adenosine (Ado), which are regulated by the enzymes nucleoside triphosphate diphosphohydrolase (NTPDase), 5'-nucleotidase, and adenosine deaminase (ADA). Thus, the aim of this study was to evaluate whether purinergic signaling can modulate the immune and inflammatory responses in the spleen of silver catfish (Rhamdia quelen) naturally infected with Ichthyophthirius multifiliis. Splenic NTPDase and 5'-nucleotidase activities increased in infected animals compared with uninfected animals, while the splenic ADA activity decreased. These data indicate that purinergic signaling can modulate the splenic immune and inflammatory responses through the regulation of ATP and Ado levels, which are known to participate in the physiological and pathological responses as pro-inflammatory and anti-inflammatory molecules, respectively. In summary, modulation of the splenic purinergic cascade has an anti-inflammatory role in reducing or preventing inflammatory damage.

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The adenosinergic system, not the cholinergic system, exerts an anti-inflammatory profile in lymphatic immune organs of fish naturally infected with Ichthyophthirius multifiliis

Cited by 9 publications

References 32 publications

Serum adenosine deaminase and xanthine oxidase activities in silver catfish naturally infected with Ichthyophthirius multifiliis: The influence of these enzymes on inflammatory and oxidative status

Serum adenosine deaminase and xanthine oxidase activities in silver catfish naturally infected with Ichthyophthirius multifiliis: The influence of these enzymes on inflammatory and oxidative status

Ichthyophthirius multifiliis impairs splenic enzymes of the phosphoryl transfer network in naturally infected Rhamdia quelen: effects on energetic homeostasis

Purinergic signaling modulates the splenic inflammatory response in silver catfish naturally infected with Ichthyophthirius multifiliis

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