The Adenosine Receptor Agonist 5’-&amp;lt;i&amp;gt;N&amp;lt;/i&amp;gt;-Ethylcarboxamide-Adenosine Increases Glucose 6-Phosphatase Expression and Gluconeogenesis

Matsuda, Koichi; Horikawa, Yoko; Sasaki, Yasuto; Sakata, Shigeko Fujimoto

doi:10.4236/pp.2014.51004

Cited by 2 publications

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References 23 publications

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“…Seven experiments were performed using the NECA treatment. Details of the NECA treatment methods have been described previously [19]. Briefly, after the fasting period, mice were intraperitoneally (i.p.)…”

Section: Animal Treatmentsmentioning

confidence: 99%

“…We previously reported that the non-specific adenosine receptor agonist 5'-N-ethylcarboxamide-adenosine (NECA) increased serum glucose levels and the expression of a glucogenic enzyme (glucose 6-phosphatase) in the liver [19] [20]. Based on the dose of NECA administered in these studies and plasma concentrations after the administration of other adenosine agonists [21], it was inferred that the serum NECA concentration was in the μM range and also that NECA activated adenosine A2b receptors.…”

Section: Introductionmentioning

confidence: 99%

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The Adenosine Receptor Agonist 5’-N-Ethylcarboxamide-Adenosine Increases Mouse Serum Total Homocysteine Levels, Which Is a Risk Factor for Cardiovascular Diseases

Sakata

Matsuda²,

Horikawa³

et al. 2015

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An increase in total homocysteine (Hcy) levels (protein-bound and free Hcy in the serum) has been identified as a risk factor for vascular diseases. Hcy is a product of the methionine cycle and is a precursor of glutathione in the transsulfuration pathway. The methionine cycle mainly occurs in the liver, with Hcy being exported out of the liver and subsequently bound to serum proteins. When the non-specific adenosine receptor agonist 5'-N-ethylcarboxamide-adenosine (NECA; 0.1 or 0.3 mg/kg body weight) was intraperitoneally administered to mice that had been fasted for 16 h, total Hcy levels in the serum significantly increased 1 h after its administration. The NECA treatment may have inhibited transsulfuration because glutathione levels were significantly decreased in the liver. After the intraperitoneal administration of a high dose of NECA (0.3 mg/kg body weight), elevations in total Hcy levels in the serum continued for up to 10 h. The mRNA expression of methionine metabolic enzymes in the liver was significantly reduced 6 h after the administration of NECA. NECA-induced elevations in total serum Hcy levels may be maintained in the long term through the attenuated expression of methionine metabolic enzymes.

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Section: Animal Treatmentsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%