The ultrastructure of the calcium-sensitive (Ca-s) (PAS-positive) cells of the pars intermedia was investigated in eels kept in hypo- and hyperosmotic environments. Although the cells were moderately active in fresh water (FW), they were highly stimulated in deionized water (DW) and displayed an enlarged Golgi apparatus, a distinct rough endoplasmic reticulum, few secretory granules, some microtubules and an extended area of contact with the basal lamina that separates nervous and glandular tissues. Some mitosing cells were seen. A similar picture was observed in eels kept in sea water (SW) for 45 days, returned to FW and subsequently to DW for 21 days. In SW (20 and 33%), and particularly in concentrated SW (50, 60 and 63%), the Ca-s cells were inactive. Their granules were significantly smaller than in eels kept in FW, and the area of contact with the basal lamina was greatly reduced. However, signs of granule-release were seen in eels adapted to 50 and 60% SW. Nerve fibers rarely contacted the Ca-s cells and did not synapse with them. The ultrastructural data support the hypothesis that the Ca-s cells of Anguilla, like those of Carassius, are involved in ionic regulation. MSH cells were not greatly affected by the present experiments.