The Addition of Platelet-Rich Plasma to Scaffolds Used for Cartilage Repair: A Review of Human and Animal Studies

Sermer, Corey; Devitt, Brian M.; Chahal, Jaskarndip; Kandel, Rita A.; Theodoropoulos, John

doi:10.1016/j.arthro.2015.01.027

Cited by 25 publications

(14 citation statements)

References 97 publications

(161 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Furthermore, the delivery of platelet-rich plasma into the joint seems to ameliorate cartilage defects; however, the molecules responsible for these chondroprotective effects remain elusive (8)(9)(10).…”

mentioning

confidence: 99%

Identification of Novel Chondroprotective Mediators in Resolving Inflammatory Exudates

Kaneva

Greco

Headland

et al. 2017

The Journal of Immunology

View full text Add to dashboard Cite

We hypothesized that exudates collected at the beginning of the resolution phase of inflammation might be enriched for tissue protective molecules; thus an integrated cellular and molecular approach was applied to identify novel chondroprotective bioactions. Exudates were collected 6 h (inflammatory) and 24 h (resolving) following carrageenan-induced pleurisy in rats. The resolving exudate was subjected to gel filtration chromatography followed by proteomics, identifying 61 proteins. Fractions were added to C28/I2 chondrocytes, grown in micromasses, ions with or without IL-1β or osteoarthritic synovial fluids for 48 h. Three proteins were selected from the proteomic analysis, α1-antitrypsin (AAT), hemopexin (HX), and gelsolin (GSN), and tested against catabolic stimulation for their effects on glycosaminoglycan deposition as assessed by Alcian blue staining, and gene expression of key anabolic proteins by real-time PCR. In an in vivo model of inflammatory arthritis, cartilage integrity was determined histologically 48 h after intra-articular injection of AAT or GSN. The resolving exudate displayed protective activities on chondrocytes, using multiple readouts: these effects were retained in low m.w. fractions of the exudate (46.7% increase in glycosaminoglycan deposition; ∼20% upregulation of COL2A1 and aggrecan mRNA expression), which reversed the effect of IL-1β. Exogenous administration of HX, GSN, or AAT abrogated the effects of IL-1β and osteoarthritic synovial fluids on anabolic gene expression and increased glycosaminoglycan deposition. Intra-articular injection of AAT or GSN protected cartilage integrity in mice with inflammatory arthritis. In summary, the strategy for identification of novel chondroprotective activities in resolving exudates identified HX, GSN and AAT as potential leads for new drug discovery programs.

show abstract

mentioning

confidence: 99%

Identification of Novel Chondroprotective Mediators in Resolving Inflammatory Exudates

Kaneva

Greco

Headland

et al. 2017

The Journal of Immunology

View full text Add to dashboard Cite

show abstract

“…In previous studies, we showed that cells contained in the micrografts derived from different tissues express mesenchymal stem cells (MSCs) markers, such as CD90, CD73 and CD105, CD117, and CD44 [ 7 , 8 , 25 ], suggesting the presence of MSCs or tissue-specific progenitors within micrografts, as a possible explanation of their regenerative potential. Behind the stem cells, also the PRP containing both PDGF and TGF-β1 was shown to be effective in cartilage regeneration by promoting chondrocyte proliferation and synthesis of proteoglycan and type II collagen [ 29 ]. We reported in this study the pre-clinical application of autologous micrografts combined with PRP in the cartilage repair in racehorses, suggesting that a combined action of both these factors would be able to promote the osteochondral regeneration.…”

Section: Discussionmentioning

confidence: 99%

“…We reported in this study the pre-clinical application of autologous micrografts combined with PRP in the cartilage repair in racehorses, suggesting that a combined action of both these factors would be able to promote the osteochondral regeneration. In fact, PRP alone resulted effectively in the treatment of cartilage lesions only at short-term [ 30 , 31 ], and it has been suggested that the combination with other approaches would allow for the stabilization of its beneficial effects [ 29 ]. Even if the known short-term effect of PRP may have masked the benefits of autologous cartilage micrografts, our in vivo data provide the first proof of concept for the use of the combination of these techniques in cartilage defects.…”

Section: Discussionmentioning

confidence: 99%

Rationale and pre-clinical evidences for the use of autologous cartilage micrografts in cartilage repair

Viganò

Tessaro

Trovato³

et al. 2018

J Orthop Surg Res

View full text Add to dashboard Cite

BackgroundThe management of cartilage lesions is an open issue in clinical practice, and regenerative medicine represents a promising approach, including the use of autologous micrografts whose efficacy was already tested in different clinical settings. The aim of this study was to characterize in vitro the effect of autologous cartilage micrografts on chondrocyte viability and differentiation and perform an evaluation of their application in racehorses affected by joint diseases.Materials and methodsMatched human chondrocytes and micrografts were obtained from articular cartilage using Rigenera® procedure. Chondrocytes were cultured in the presence or absence of micrografts and chondrogenic medium to assess cell viability and cell differentiation. For the pre-clinical evaluation, three racehorses affected by joint diseases were treated with a suspension of autologous micrografts and PRP in arthroscopy interventions. Clinical and radiographic follow-ups were performed up to 4 months after the procedure.ResultsAutologous micrografts support the formation of chondrogenic micromasses thanks to their content of matrix and growth factors, such as transforming growth factor β (TGFβ) and insulin-like growth factor 1 (IGF-1). On the other hand, no significant differences were observed on the gene expression of type II collagen, aggrecan, and SOX9. Preliminary data in the treatment of racehorses are suggestive of a potential in vivo use of micrografts to treat cartilage lesions.ConclusionThe results reported in this study showed the role of articular micrografts in the promoting chondrocyte differentiation suggesting their potential use in the clinical practice to treat articular lesions.Electronic supplementary materialThe online version of this article (10.1186/s13018-018-0983-y) contains supplementary material, which is available to authorized users.

show abstract

“…Their relatively larger joint size and thicker cartilages, compared with rats and mice, would increase their usefulness significantly. Full-or half-thickness cartilage defect models could be constructed in rabbits [47][48][49], with the subchondral bone being involved in 90 % of cases. The main limitation of rabbit models includes spontaneous healing potential, with rabbit cartilage possessing greater intrinsic healing capacity than larger animals such as horses and humans [50,51].…”

Section: In Vivo Modelmentioning

confidence: 99%

Perspectives on Animal Models Utilized for the Research and Development of Regenerative Therapies for Articular Cartilage

et al. 2016

View full text Add to dashboard Cite

Animal models are integral and indispensable for biomedical research and regenerative medicine studies, as these provide invaluable information for systemically evaluating the potential risks and efficacy of newly developed biomaterials, drugs, medical devices, and therapeutic modalities, prior to initiation of human clinical trials. Nevertheless, it is important to be aware of the unique strengths and limitations of the various different small and large animal models commonly utilized for biomedical research as well as the various challenges faced in extrapolating results acquired from animal studies and the risks of data misinterpretation. This review will thus critically examine various animal models utilized for studies on articular cartilage regeneration. Particular emphasis will be placed on comparing and analyzing the unique strengths and limitations of each animal model, with the aim of establishing principles for evaluating the suitability of different animal models for individual studies as well as for comprehensive interpretation and extrapolation of results obtained from various animal species. Additionally, this review will also discuss to evaluate animal studies with in situ imaging techniques, how the animal genome may result in variability in experimental outcomes, as well as the contribution of animal models to the development of cartilage tissue engineering.

show abstract

The Addition of Platelet-Rich Plasma to Scaffolds Used for Cartilage Repair: A Review of Human and Animal Studies

Cited by 25 publications

References 97 publications

Identification of Novel Chondroprotective Mediators in Resolving Inflammatory Exudates

Identification of Novel Chondroprotective Mediators in Resolving Inflammatory Exudates

Rationale and pre-clinical evidences for the use of autologous cartilage micrografts in cartilage repair

Perspectives on Animal Models Utilized for the Research and Development of Regenerative Therapies for Articular Cartilage

Contact Info

Product

Resources

About