The addition of accessory enzymes enhances the hydrolytic performance of cellulase enzymes at high solid loadings

A comparative study on enzymatic hydrolysis of kenaf from two different harvest time-points, with- and without pretreatment

“…As previous work had shown that higher proportion of xylanase was required when the substrate had a relatively high amount of xylose (Hu et al, 2015). In this study, the conversion of xylose was not increased because low amount of xylanase was loaded on to biomass.…”

Accessory enzymes influence cellulase hydrolysis of the model substrate and the realistic lignocellulosic biomass

“…Since the accessory enzyme that cannot be addressed with the FPA assay had such a positive role on lignocellulosic hydrolysis, it is possible to verify it by supplementation of some accessory enzymes during cellulase hydrolysis of pretreated lignocellulsic substrate [7,8,26]. Therefore, some leading accessory enzymes such as endoxylanase, β-xylosidase and AA9 were directly added into the CTec1 cellulase hydrolysis of SPSSB.…”

“…Recent work has shown how important the role that the so-called accessory enzymes can play in the effective enzymatic hydrolysis [5][6][7][8]. These accessory enzyme activities can improve the performance of enzyme preparations even when not directly involved in the hydrolysis of cellulose.…”

FgLPMO9A from Fusarium graminearum cleaves xyloglucan independently of the backbone substitution pattern

“…important ingredient in commercial enzyme cocktails for industrial biomass conversion, such as Cellic CTec2 [24], owing to their synergy with GHs in improving saccharification yields [25,26].…”