The added value of a commercial 16S/18S-PCR assay (UMD-SelectNA, Molzym) for microbiological diagnosis of spondylodiscitis: an observational study

Both, Anna; Martin, Coralie; Berinson, Benjamin; Dreimann, Marc; Viezens, Lennart; Lütgehetmann, Marc; Aepfelbacher, Martin; Rohde, Holger; Stangenberg, Martin

doi:10.1016/j.diagmicrobio.2023.115926

Cited by 1 publication

(1 citation statement)

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“…Moreover, because of its capacity to detect low levels of bacterial DNA in clinical samples, 16S NGS has proved a powerful tool in the identification of difficult-to-grow bacteria. These advantages offer more accurate and comprehensive information to healthcare professionals, leading to more informed decisions and better management of patients in several settings [5][6][7], such as periprosthetic infections [8], osteomyelitis [9], and endocarditis [10]. In this regard, the latest version of the guidelines from the European Society of Cardiology recommends the use of molecular biology techniques to improve the diagnosis of culture-negative endocarditis [11].…”

Section: Introductionmentioning

confidence: 99%

Performance of 16S rRNA Gene Next-Generation Sequencing and the Culture Method in the Detection of Bacteria in Clinical Specimens

Botan,

Campisciano,

Zerbato

et al. 2024

Diagnostics

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Effective treatment of infectious diseases requires prompt and accurate bacterial identification and tailored antimicrobial treatments. Traditional culture methods are considered the gold standard, but their effectiveness diminishes for fastidious and hard-to-grow microorganisms. In recent years, molecular diagnostic tools such as 16S rRNA gene next-generation sequencing (16S NGS) have gained popularity in the field. We analysed data from samples submitted for 16S NGS between July 2022 and July 2023 at the Department of Advanced Translational Microbiology in Trieste, Italy. The study included samples submitted for both culture-based identification and 16S NGS. Conventional media were used for culture, and bacterial identification was performed using MALDI-TOF mass spectrometry. The V3 region of the 16S rRNA gene was sequenced using the Ion PGM platform. Among the 123 samples submitted, drainage fluids (38%) and blood (23%) were the most common, with requests predominantly from the Infectious Diseases (31.7%) and Orthopedic (21.13%) Units. In samples collected from patients with confirmed infections, 16S NGS demonstrated diagnostic utility in over 60% of cases, either by confirming culture results in 21% or providing enhanced detection in 40% of instances. Among the 71 patients who had received antibiotic therapies before sampling (mean 2.3 prior antibiotic days), pre-sampling antibiotic consumption did not significantly affect the sensitivity of 16S NGS. In routine microbiology laboratories, combining 16S NGS with culture method enhances the sensitivity of microbiological diagnostics, even when sampling is conducted during antibiotic therapy.

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