The ADAPTOR PROTEIN-3 Complex Mediates Pollen Tube Growth by Coordinating Vacuolar Targeting and Organization

Feng, Qiang-Nan; Xin, Liang; Li, Sha

doi:10.1104/pp.17.01722

Cited by 28 publications

(25 citation statements)

References 48 publications

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“…Important for Stomatal Closure. AP3M is involved in regulating membrane trafficking (24,27,28), vacuole morphology (25,26), germination and postgermination development (29,30), and pollen tube growth (55) in Arabidopsis. Our results demonstrate that the AP3 complex mediates the sorting of cargoes to the vacuole and regulates vacuole morphology in guard cells; importantly, this regulatory process requires the F-actin binding activity of AP3M.…”

Section: Protein Sorting To the Vacuole And Vacuole Morphology Arementioning

confidence: 99%

AP3M harbors actin filament binding activity that is crucial for vacuole morphology and stomatal closure in Arabidopsis

Zheng

Jiang

Wang

et al. 2019

Proc. Natl. Acad. Sci. U.S.A.

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Stomatal movement is essential for plant growth. This process is precisely regulated by various cellular activities in guard cells. F-actin dynamics and vacuole morphology are both involved in stomatal movement. The sorting of cargoes by clathrin adaptor protein (AP) complexes from the Golgi to the vacuole is critical for establishing a normal vacuole morphology. In this study, we demonstrate that the medium subunit of the AP3 complex (AP3M) binds to and severs actin filaments in vitro and that it participates in the sorting of cargoes (such as the sucrose exporter SUC4) to the tonoplast, and thereby regulates stomatal closure in Arabidopsis thaliana. Defects in AP3 or SUC4 led to more rapid water loss and delayed stomatal closure, as well as hypersensitivity to drought stress. In ap3m mutants, the F-actin status was altered compared to the wild type, and the sorted cargoes failed to localize to the tonoplast. AP3M contains a previously unidentified F-actin binding domain that is conserved in AP3M homologs in both plants and animals. Mutations in the F-actin binding domain of AP3M abolished its F-actin binding activity in vitro, leading to an aberrant vacuole morphology and reduced levels of SUC4 on the tonoplast in guard cells. Our findings indicate that the F-actin binding activity of AP3M is required for the precise localization of AP3-dependent cargoes to the tonoplast and for the regulation of vacuole morphology in guard cells during stomatal closure.

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Section: Protein Sorting To the Vacuole And Vacuole Morphology Arementioning

confidence: 99%

AP3M harbors actin filament binding activity that is crucial for vacuole morphology and stomatal closure in Arabidopsis

Zheng

Jiang

Wang

et al. 2019

Proc. Natl. Acad. Sci. U.S.A.

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“…Adaptor proteins that are involved in protein trafficking and sorting [87][88][89] may recognize cargo and coat proteins during vesicle formation [90]. AP-3 was first identified in mammalian cells and likely functions as a clathrin adaptor [91]; losses in AP-3 function reduce seed germination potential [92,93], due to mistargeted protein S-ACYL transferase10 that is critical for pollen tube growth during dynamic vacuolar organization in Arabidopsis [94].…”

Section: Annotation Of Candidate Snpmentioning

confidence: 99%

QTL-Seq Identifies QTL for Relative Electrical Conductivity Associated with Heat Tolerance in Bottle Gourd (Lagenaria Siceraria)

Song

Huang

2019

Preprint

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Heat is a major abiotic stressor seriously affects watermelon produce; however, its effects may be mitigated through grafting onto heat tolerant bottle gourd rootstock. To understand the genetic basis and select reliable markers are necessary in the variety breeding process. In this paper, the relative electric conductivity (REC) was used as a visual indicator for heat stress to study the genetics and SNP marker of heat tolerance in bottle gourd via QTL-seq approach. The results showed that recessive inheritance and a major QTL locus controlled REC related to heat tolerance. Seven heat-tolerant loci (qHT1. 1, qHT2.1, qHT2.2, qHT5.1, qHT6.1, qHT7.1, and qHT8.1) exhibited high Δ(SNP-index) values (ranging from 0.19-0.38), and of these, the greatest value (0.32, P < 0.01) and greatest number of detected SNPs (9052) were found in chromosome 2 at qHT2.1 region (11.03 -19.25 Mb); qHT2.1was taken as a promising major QTL for heat tolerance in bottle gourd. A total of 34 putative candidate genes related to heat stress were detected within the qHT2.1 region. Three polymorphic SNPs (BG_GLEAN_10022642, BG_GLEAN_10022727, and BG_GLEAN_10022589) that are involved in pollen sterility, intracellular transport, and signal recognition were validated and exhibited significant marker-trait association that could be used in heat tolerant molecular breeding for bottle gourd. The qHT2.1region is an important finding can be used for fine mapping and discovering new genes associated with heat tolerance in bottle gourd.

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“…Heterotetrameric adaptor protein (AP) complexes are key components of protein sorting in endocytic and post-Golgi secretory pathways (reviewed by Canagarajah et al, 2013). Genetic approaches in Arabidopsis have revealed that AP complexes function in hormone signaling (Di Rubbo et al, 2013;Fan et al, 2013;Kansup et al, 2013;Wang et al, 2013), cytokinesis (Teh et al, 2013), gravitropism (Niihama et al 2009), reproduction Yamaoka et al, 2013;Feng et al, 2018), and immunity (Hatsugai et al, 2016). AP1 mediates protein transport from the TGN/EE to the plasma membrane, the cell plate and the vacuole (Park et al, 2013;Teh et al, 2013;.…”

Section: Introductionmentioning

confidence: 99%

Sorting motifs target the movement protein of ourmia melon virus to the trans-Golgi network and plasmodesmata

Ozber

Margaria

Anderson

et al. 2019

Preprint

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Plants have a highly sophisticated endomembrane system that is targeted by plant viruses for cell-to-cell movement. The movement protein (MP) of Ourmia melon virus (OuMV) is targeted to plasmodesmata (PD) and forms tubules to facilitate cell-to-cell movement. Despite a number of functionally important regions for correct subcellular localization of OuMV MP has been identified, little is known about the pathways OuMV MP hijacks to reach PD. Here, we demonstrate that OuMV MP localizes to the trans-Golgi network (TGN), but not to the multivesicular body/prevacuolar compartment or Golgi, and carries two putative sorting motifs, a tyrosine (Y) and a dileucine (LL) motif, near its N-terminus. Introducing glycine substitutions in these motifs results in loss of OuMV infectivity in Nicotiana benthamiana and Arabidopsis (Arabidopsis thaliana). Live cell imaging of GFP-labeled sorting motif mutants shows that Y motif mutants fail to localize to the TGN, plasma membrane, and PD. Mutations in the LL motif do not impair plasma membrane targeting of MP, but affect its ability to associate with callose deposits at PD. Taken together, these data suggest that both Y and LL motifs are indispensable for targeting of OuMV MP to PD and for efficient systemic infection, but show differences in functionality. This study provides new insights into the role of sorting motifs in intracellular targeting of MPs and vesicle trafficking pathways that plant viruses hijack for cell-to-cell movement.

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The ADAPTOR PROTEIN-3 Complex Mediates Pollen Tube Growth by Coordinating Vacuolar Targeting and Organization

Cited by 28 publications

References 48 publications

AP3M harbors actin filament binding activity that is crucial for vacuole morphology and stomatal closure in Arabidopsis

AP3M harbors actin filament binding activity that is crucial for vacuole morphology and stomatal closure in Arabidopsis

QTL-Seq Identifies QTL for Relative Electrical Conductivity Associated with Heat Tolerance in Bottle Gourd (Lagenaria Siceraria)

Sorting motifs target the movement protein of ourmia melon virus to the trans-Golgi network and plasmodesmata

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