The adaptive effects of dietary fish and safflower oil on lipid and lipoprotein metabolism in perfused rat liver

Wong, Sue; Nestel, Paul J.; Trimble, Rodney P.; Storer, Gerald B.; Illman, Richard J.; Topping, David L.

doi:10.1016/0005-2760(84)90209-1

Cited by 297 publications

(115 citation statements)

References 39 publications

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102

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“…In contrast to our earlier findings with the perfused rat liver [4], both oils inhibited hepatic fatty acid synthesis to the same extent (table 3). This lack of difference probably reflects the higher plasma free fatty acid concentrations found with safflower oil feeding [2].…”

Section: Discussioncontrasting

confidence: 99%

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The effects of dietary fish oil on hepatic high density and low density lipoprotein receptor activities in the rat

et al. 1987

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Rats were fed either a standard ration diet or that diet supplemented with 8% by wt of a marine fish oil or safflower oil. After 10 days, plasma triacylglycerols, total cholesterol, high density lipoprotein (HDL) cholesterol, hepatic cholesterol and fatty acid synthesis and hepatic low density lipoprotein (LDL) receptor activity were significantly depressed while HDL receptor activity was significantly increased in rats fed fish oil. Fish oil-induced effects on cholesterol metabolism in the rat therefore include reciprocal changes in the activities of hepatic LDL and HDL receptors.

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Section: Discussioncontrasting

confidence: 99%

“…This lack of difference probably reflects the higher plasma free fatty acid concentrations found with safflower oil feeding [2]. The greater direct inhibitory effects of these fatty acids [16] are likely to compensate for the greater adaptive downregulating effects [4] of fish oil on the hepatic enzymes involved in fatty acid synthesis.…”

Section: Discussionmentioning

confidence: 99%

The effects of dietary fish oil on hepatic high density and low density lipoprotein receptor activities in the rat

et al. 1987

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“…We have also recently observed a similar reciprocal relationship between the LDL and HDL receptor activities in the livers of rats fed fish oil [26]. The concentration of unesterified cholesterol in the livers of these rats may well rise because the production and secretion of VLDL [27] and therefore the secretion of cholesterol is severely inhibited by the dietary oils. The livers of the fish oil fed-rats, like the Hep G2 cells incubated with cholesterol, probably attempt to decrease the concentration of unesterified cholesterol by down-regulating their LDL receptors to reduce the uptake of cholesterol-rich LDL [28].…”

Section: Discussionsupporting

confidence: 53%

Demonstration of a high density lipoprotein (HDL)‐binding protein in Hep G2 cells using colloidal gold‐HDL conjugates

1988

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Solubilized membrane proteins of Hep G2 cells were electrophoretically separated on polyacrylamide gels and electrotransferred onto nitrocellulose paper. Overlaying the nitrocellulose with human high density lipoproteins conjugated to colloidal gold revealed the presence of a single protein band with an apparent molecular mass of 80 kDa. Binding of the conjugates to this protein was specific for high density lipoproteins in as much as it was effectively displaced by an excess of unlabelled high density lipoproteins but not by a similar excess of unlabelled low density lipoproteins. Binding was not dependent on Ca*+ as 10 mM EDTA had no effect. The binding activity of the solubilized membranes was increased by incubating the cells with non-lipoprotein cholesterol. This was detected on electroblots and quantified with a new dot blot assay using the colloidal gold-high density lipoprotein conjugates.

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“…Although no significant difference in FAS expression was observed between dietary lipid groups, broilers fed fish oil diets showed a trend toward down-regulated FAS gene expression, further indicating that fish oil reduced hepatic synthesis of fatty acids. Previous study also demonstrated reduced lipogenesis with dietary fish oil (Wong et al, 1984). Studies found that fish oil inhibited lipid synthesis by decreasing the expression of FAS genes in the liver of mice (Ren et al, 1997;Kim et al, 1998).…”

Section: Discussionmentioning

confidence: 76%

Effects of dietary lipids and Clostridium butyricum on serum lipids and lipid-related gene expression in broiler chickens

Zhang

Yang

Guo

et al. 2011

Animal

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The effect of dietary lipids and Clostridium butyricum supplementation on circulating lipids and the transcription of selected genes involved in lipid metabolism were investigated in broiler chickens. One hundred and sixty-day-old broilers (Arbor Acres) were divided into four treatment groups in a 2 3 2 factorial arrangement and fed four diets with two lipid sources (soybean oil or fish oil) and two levels of C. butyricum (0 or 5 g/kg) for a period of 6 weeks. Serum concentrations of total cholesterol and low-density lipoprotein cholesterol were lower (P , 0.05) in broilers fed diets containing fish oil than in those fed diets containing soybean oil. Fish oil significantly reduced (P , 0.05) hepatic fatty acid synthase and lipoprotein lipase (LPL) activities in abdominal fat. In contrast, fish oil significantly increased (P , 0.05) breast muscle LPL activity and hepatic peroxisome proliferator-activated receptor-a and LPL gene expression. The addition of C. butyricum significantly increased (P , 0.05) LPL activity in abdominal fat and liver-type fatty acid-binding protein gene expression in jejunal mucosa. The results of this study indicated that the reduced abdominal fat in broilers fed fish oil as observed may be due to augmented hepatic fatty acid catabolism and lower hepatic fat synthesis. The increased intramuscular fat content in breast muscle of broilers fed C. butyricum as described may be the result of enhanced fatty acid uptake.

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The adaptive effects of dietary fish and safflower oil on lipid and lipoprotein metabolism in perfused rat liver

Cited by 297 publications

References 39 publications

The effects of dietary fish oil on hepatic high density and low density lipoprotein receptor activities in the rat

The effects of dietary fish oil on hepatic high density and low density lipoprotein receptor activities in the rat

Demonstration of a high density lipoprotein (HDL)‐binding protein in Hep G2 cells using colloidal gold‐HDL conjugates

Effects of dietary lipids and Clostridium butyricum on serum lipids and lipid-related gene expression in broiler chickens

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