The acute lethality and endocrine effect of 1,2,3,7,8-PeCDD in juvenile goldfish (Carassius auratus) in vivo

Yang, Yuesuo; Liu, Zhengtao; Zheng, Minghui; Zhao, Yanyan; Wang, Liang; Sun, Wei; Xiao, Feng

doi:10.1016/s1001-0742(08)60037-6

Cited by 4 publications

(2 citation statements)

References 39 publications

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“…14 ng mL -1 ) (Lahr et al 2006) and generally comparable with the range of LOD values (i.e. 6.9-62 ng mL -1 ) detected in different ELISAs for measuring Vtg in other fish species (Brion et al 2000;Holbech et al 2001;Nilsen et al 2004;Palermo et al 2008;Yang et al 2008;Wang et al 2017a). In addition, our results are also consistent with the practical detection limits (PDLs) reported for plasma Vtg of rainbow trout (i.e.…”

Section: Elisa and Optical Immunosensorsupporting

confidence: 71%

Identification, partial characterization, and use of grey mullet (Mugil cephalus) vitellogenins for the development of ELISA and biosensor immunoassays

et al. 2019

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Vitellogenin (Vtg) has proven to be a sensitive and simple biomarker in determining sex, sexual maturity, and xenoestrogenic effects in fish. Thus, our investigation has been focused on identification, partial characterization, and quantification of grey mullet (Mugil cephalus) Vtg through the use of a variety of biochemical and immunological analytical techniques. Mullet is considered both a promising aquaculture candidate and an important species for improving sediment quality in polyculture systems. In the first part of this work, grey mullet Vtg was purified from plasma of 17b-estradiol (E2)-induced male fish by a one-step chromatographic protocol, and partially characterized. Specific polyclonal antibodies were then raised against the mullet Vtg, and both an indirect ELISA and an optical immunosensor were set up and validated to quantify plasma Vtg. The indirect ELISA and the optical immunosensor assay developed showed linear measuring in the range 56.8-1047.1 ng mL-1 and 70-739 ng mL-1 Vtg concentrations in standard solutions, respectively. The results obtained suggest that the indirect ELISA allows Vtg detection over a wide dynamic range, thus resulting more suitable for rapid and sensitive sample screening. Therefore, we suggest that the direct immunosensor is a promising tool which needs more investigation to improve the sensitivity.

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Section: Elisa and Optical Immunosensorsupporting

confidence: 71%

Identification, partial characterization, and use of grey mullet (Mugil cephalus) vitellogenins for the development of ELISA and biosensor immunoassays

et al. 2019

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show abstract

“…Previous studies have mainly focused on their reproductive toxicity (Sha et al, 2015), neurotoxicity (Kodavanti and Tilson, 1997), immunotoxicity (Smialowicz et al, 1989), endocrine-interference effects (Yang et al, 2008) and oncogenicity (Lucena et al, 2001;Wolff et al, 2000). However, their potential influence on element metabolism remains largely unknown.…”

Section: Polychlorinated Biphenylsmentioning

confidence: 99%

Disruption of iron homeostasis and resultant health effects upon exposure to various environmental pollutants: A critical review

Guo

Zhang

et al. 2015

Journal of Environmental Sciences

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Preparation of a polyclonal antibody against goldfish (Carassius auratus) vitellogenin and its application to detect the estrogenic effects of monocrotophos pesticide

Wang

Bing

et al. 2015

Ecotoxicology and Environmental Safety

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The acute lethality and endocrine effect of 1,2,3,7,8-PeCDD in juvenile goldfish (Carassius auratus) in vivo

Cited by 4 publications

References 39 publications

Identification, partial characterization, and use of grey mullet (Mugil cephalus) vitellogenins for the development of ELISA and biosensor immunoassays

Identification, partial characterization, and use of grey mullet (Mugil cephalus) vitellogenins for the development of ELISA and biosensor immunoassays

Disruption of iron homeostasis and resultant health effects upon exposure to various environmental pollutants: A critical review

Preparation of a polyclonal antibody against goldfish (Carassius auratus) vitellogenin and its application to detect the estrogenic effects of monocrotophos pesticide

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